A synteny-based draft genome sequence of the forage grass Lolium perenne

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DOI

  • Stephen Byrne, Denmark
  • Istvan Nagy
  • Matthias Pfeifer, Plant Genome and Systems Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Germany
  • Ian Armstead, Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, United Kingdom
  • Suresh Swain, Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, United Kingdom
  • Bruno Studer, Institute of Agricultural Sciences, ETH Zürich, Switzerland
  • Klaus Mayer, Plant Genome and Systems Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Germany
  • Jacqueline D Campbell, Department of Agronomy, Iowa State University, United States
  • Adrian Czaban, Denmark
  • Stephan Hentrup
  • Frank Panitz
  • Christian Bendixen
  • Jakob Hedegaard
  • Mario Caccamo, The Genome Analysis Centre, Norwich Research Park, United Kingdom
  • Torben Asp
Here we report the draft genome sequence of perennial ryegrass (Lolium perenne), an economically important forage and turf grass species that is widely cultivated in temperate regions worldwide. It is classified along with wheat, barley, oats and Brachypodium distachyon in the Pooideae sub-family of the grass family (Poaceae). Transcriptome data was used to identify 28 455 gene models, and we utilized macro-co-linearity between perennial ryegrass and barley, and synteny within the grass family, to establish a synteny-based linear gene order. The gametophytic self-incompatibility mechanism enables the pistil of a plant to reject self-pollen and therefore promote out-crossing. We have used the sequence assembly to characterize transcriptional changes in the stigma during pollination with both compatible and incompatible pollen. Characterization of the pollen transcriptome identified homologs to pollen allergens from a range of species, many of which were expressed to very high levels in mature pollen grains, and are potentially involved in the self-incompatibility mechanism. The genome sequence provides a valuable resource for future breeding efforts based on genomic prediction, and will accelerate the development of new varieties for more productive grasslands.
Original languageEnglish
JournalThe Plant Journal
Volume84
Issue4
Pages (from-to)816-826
Number of pages11
ISSN0960-7412
DOIs
Publication statusPublished - 2015

    Research areas

  • Lolium perenne, perennial ryegrass, genome sequence, self-incompatability, pollen allergens

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