A Suppressor Mutation Partially Reverts the xantha Trait via Lowered Methylation in the Promoter of Genomes Uncoupled 4 in Rice

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A Suppressor Mutation Partially Reverts the xantha Trait via Lowered Methylation in the Promoter of Genomes Uncoupled 4 in Rice. / Jiang, Meng; Liu, Yanhua; Li, Ruiqing; Zheng, Yunchao; Fu, Haowei; Tan, Yuanyuan; Moller, Ian Max; Fan, Longjiang; Shu, Qingyao; Huang, Jianzhong.

In: Frontiers in Plant Science, Vol. 10, 1003, 08.2019.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Jiang, M, Liu, Y, Li, R, Zheng, Y, Fu, H, Tan, Y, Moller, IM, Fan, L, Shu, Q & Huang, J 2019, 'A Suppressor Mutation Partially Reverts the xantha Trait via Lowered Methylation in the Promoter of Genomes Uncoupled 4 in Rice', Frontiers in Plant Science, vol. 10, 1003. https://doi.org/10.3389/fpls.2019.01003

APA

Jiang, M., Liu, Y., Li, R., Zheng, Y., Fu, H., Tan, Y., ... Huang, J. (2019). A Suppressor Mutation Partially Reverts the xantha Trait via Lowered Methylation in the Promoter of Genomes Uncoupled 4 in Rice. Frontiers in Plant Science, 10, [1003]. https://doi.org/10.3389/fpls.2019.01003

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Jiang, Meng ; Liu, Yanhua ; Li, Ruiqing ; Zheng, Yunchao ; Fu, Haowei ; Tan, Yuanyuan ; Moller, Ian Max ; Fan, Longjiang ; Shu, Qingyao ; Huang, Jianzhong. / A Suppressor Mutation Partially Reverts the xantha Trait via Lowered Methylation in the Promoter of Genomes Uncoupled 4 in Rice. In: Frontiers in Plant Science. 2019 ; Vol. 10.

Bibtex

@article{e19027e0ad88420581d162a02a458429,
title = "A Suppressor Mutation Partially Reverts the xantha Trait via Lowered Methylation in the Promoter of Genomes Uncoupled 4 in Rice",
abstract = "The xantha trait of a yellow leaf rice mutant (HYB), controlled epigenetically by elevated CHG methylation of the genomes uncoupled 4 (OsGUN4) promoter, has reduced chlorophyll content, altered tetrapyrrole biosynthesis, and deregulated transcription of photosynthesis-associated nuclear genes (PhANGs) compared to its wild-type progenitor Longtefu B (LTB). In the present study, we identified a suppressor mutant (CYB) of HYB and characterized its genetic, molecular, and physiological basis of the mutant phenotype. We found that the light-green phenotype of CYB was caused by a suppressor mutation in an unknown gene other than OsGUN4. Compared to HYB, the CHG methylation in the OsGUN4 promoter was reduced, while OsGUN4 transcript and protein abundance levels were increased in CYB. The contents of total chlorophyll and its intermediate metabolites (except protoporphyrin IX) in CYB plants were intermediate between HYB and LTB. The expression levels of 30 genes involved in tetrapyrrole biosynthesis in CYB were all partially reverted to those of LTB, so were the PhANGs. In summary, a suppressor mutation caused the reversion of the xantha trait via reducing CHG methylation in OsGUN4 promoter.",
keywords = "chlorophyll, DNA methylation, epigenetics, genomes uncoupled 4, photosynthesis, tetrapyrrole, rice (Oryza sativa), TETRAPYRROLE BIOSYNTHESIS, MG-CHELATASE, MAGNESIUM CHELATASE, MARKER TRAIT, GUN4 PROTEIN, GIBBERELLIN, MUTANT, SUBUNIT, IDENTIFICATION, MECHANISM",
author = "Meng Jiang and Yanhua Liu and Ruiqing Li and Yunchao Zheng and Haowei Fu and Yuanyuan Tan and Moller, {Ian Max} and Longjiang Fan and Qingyao Shu and Jianzhong Huang",
year = "2019",
month = "8",
doi = "10.3389/fpls.2019.01003",
language = "English",
volume = "10",
journal = "Frontiers in Plant Science",
issn = "1664-462X",
publisher = "Frontiers Media S.A",

}

RIS

TY - JOUR

T1 - A Suppressor Mutation Partially Reverts the xantha Trait via Lowered Methylation in the Promoter of Genomes Uncoupled 4 in Rice

AU - Jiang, Meng

AU - Liu, Yanhua

AU - Li, Ruiqing

AU - Zheng, Yunchao

AU - Fu, Haowei

AU - Tan, Yuanyuan

AU - Moller, Ian Max

AU - Fan, Longjiang

AU - Shu, Qingyao

AU - Huang, Jianzhong

PY - 2019/8

Y1 - 2019/8

N2 - The xantha trait of a yellow leaf rice mutant (HYB), controlled epigenetically by elevated CHG methylation of the genomes uncoupled 4 (OsGUN4) promoter, has reduced chlorophyll content, altered tetrapyrrole biosynthesis, and deregulated transcription of photosynthesis-associated nuclear genes (PhANGs) compared to its wild-type progenitor Longtefu B (LTB). In the present study, we identified a suppressor mutant (CYB) of HYB and characterized its genetic, molecular, and physiological basis of the mutant phenotype. We found that the light-green phenotype of CYB was caused by a suppressor mutation in an unknown gene other than OsGUN4. Compared to HYB, the CHG methylation in the OsGUN4 promoter was reduced, while OsGUN4 transcript and protein abundance levels were increased in CYB. The contents of total chlorophyll and its intermediate metabolites (except protoporphyrin IX) in CYB plants were intermediate between HYB and LTB. The expression levels of 30 genes involved in tetrapyrrole biosynthesis in CYB were all partially reverted to those of LTB, so were the PhANGs. In summary, a suppressor mutation caused the reversion of the xantha trait via reducing CHG methylation in OsGUN4 promoter.

AB - The xantha trait of a yellow leaf rice mutant (HYB), controlled epigenetically by elevated CHG methylation of the genomes uncoupled 4 (OsGUN4) promoter, has reduced chlorophyll content, altered tetrapyrrole biosynthesis, and deregulated transcription of photosynthesis-associated nuclear genes (PhANGs) compared to its wild-type progenitor Longtefu B (LTB). In the present study, we identified a suppressor mutant (CYB) of HYB and characterized its genetic, molecular, and physiological basis of the mutant phenotype. We found that the light-green phenotype of CYB was caused by a suppressor mutation in an unknown gene other than OsGUN4. Compared to HYB, the CHG methylation in the OsGUN4 promoter was reduced, while OsGUN4 transcript and protein abundance levels were increased in CYB. The contents of total chlorophyll and its intermediate metabolites (except protoporphyrin IX) in CYB plants were intermediate between HYB and LTB. The expression levels of 30 genes involved in tetrapyrrole biosynthesis in CYB were all partially reverted to those of LTB, so were the PhANGs. In summary, a suppressor mutation caused the reversion of the xantha trait via reducing CHG methylation in OsGUN4 promoter.

KW - chlorophyll

KW - DNA methylation

KW - epigenetics

KW - genomes uncoupled 4

KW - photosynthesis

KW - tetrapyrrole

KW - rice (Oryza sativa)

KW - TETRAPYRROLE BIOSYNTHESIS

KW - MG-CHELATASE

KW - MAGNESIUM CHELATASE

KW - MARKER TRAIT

KW - GUN4 PROTEIN

KW - GIBBERELLIN

KW - MUTANT

KW - SUBUNIT

KW - IDENTIFICATION

KW - MECHANISM

U2 - 10.3389/fpls.2019.01003

DO - 10.3389/fpls.2019.01003

M3 - Journal article

VL - 10

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

SN - 1664-462X

M1 - 1003

ER -