A single-stranded architecture for cotranscriptional folding of RNA nanostructures

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  • Cody Geary
  • Paul Rothemund, Bioengineering, Computer Science, and Computation and Neural Systems, California Institute of Technology, Pasadena, CA 91125, USA., United States
  • Ebbe Sloth Andersen
Artificial DNA and RNA structures have been used as scaffolds for a variety of nanoscale devices. In comparison to DNA structures, RNA structures have been limited in size, but they also have advantages: RNA can fold during transcription and thus can be genetically encoded and expressed in cells. We introduce an architecture for designing artificial RNA structures that fold from a single strand, in which arrays of antiparallel RNA helices are precisely organized by RNA tertiary motifs and a new type of crossover pattern. We constructed RNA tiles that assemble into hexagonal lattices and demonstrated that lattices can be made by annealing and/or cotranscriptional folding. Tiles can be scaled up to 660 nucleotides in length, reaching a size comparable to that of large natural ribozymes
Original languageEnglish
JournalScience
Volume345
Issue6198
Pages (from-to)799-804
ISSN0036-8075
DOIs
Publication statusPublished - 15 Aug 2014

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