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A single hemoglobin gene in Myrica gale retains both symbiotic and non-symbiotic specificity

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  • Department of Molecular Biology
Here, a hemoglobin gene from the nitrogen-fixing actinorhizal plant Myrica gale was isolated, cloned and sequenced. The gene (MgHb) was a class I hemoglobin with strong sequence homology to non-symbiotic hemoglobin genes. MgHb is highly expressed in symbiotic root nodules, but transcripts and protein were also detected in leaves of M. gale. In Arabidopsis thaliana the MgHb promoter, linked to a beta-glucuronidase coding region, directed expression in the vascular tissue, in shoot meristem and at root branch point--a pattern very similar to the combined expression pattern of the two non-symbiotic A. thaliana hemoglobin promoters AHb1 and AHb2. The results points to a symbiotic as well as a non-symbiotic specificity of MgHb similar to a hemoglobin gene identified in Parasponia andersonii, but different from the situation in Casuarina glauca--a close actinorhizal relative of M. gale.
Original languageEnglish
JournalPlant Molecular Biology
Volume61
Issue4-5
Pages (from-to)769-79
Number of pages10
ISSN0167-4412
DOIs
Publication statusPublished - 2006

    Research areas

  • Arabidopsis, Cloning, Molecular, Ethylenes, Gene Expression Regulation, Plant, Hemoglobins, Myrica, Phylogeny, Plant Leaves, Plant Proteins, Plant Roots, Plant Stems, Plants, Genetically Modified, Promoter Regions, Genetic, Symbiosis

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