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A Set of Lotus japonicus Gifu x Lotus burttii Recombinant Inbred Lines Facilitates Map-based Cloning and QTL Mapping

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  • Niels Sandal
  • Haojie Jin, Denmark
  • Dulce Nombre Rodriguez-Navarro, Centro Las Torres-Tomejil (IFAPA), Spain
  • Francisco Temprano, Centro Las Torres-Tomejil (IFAPA), Spain
  • Cristina Cvitanich, Denmark
  • Andreas Brachmann, LMU München, Biozentrum Martinsried, Germany
  • Shusei Sato, Kazusa DNA Research Institute, Japan
  • Masayoshi Kawaguchi, Division of Symbiotic Systems, National Institute for Basic Biology, National Institute for Natural Sciences, Japan
  • Satoshi Tabata, Kazusa DNA Research Institute, Japan
  • Martin Parniske, LMU München, Biozentrum Martinsried, Germany
  • Jose E Ruiz-Sainz, 3. Dpto. de Microbiologia, Facultad de Biologia, Universidad de Sevilla, Spain
  • Stig Uggerhøj Andersen
  • Jens Stougaard
Model legumes such as Lotus japonicus have contributed significantly to the understanding of symbiotic nitrogen fixation. This insight is mainly a result of forward genetic screens followed by map-based cloning to identify causal alleles. The L. japonicus ecotype 'Gifu' was used as a common parent for inter-accession crosses to produce F2 mapping populations either with other L. japonicus ecotypes, MG-20 and Funakura, or with the related species L. filicaulis. These populations have all been used for genetic studies but segregation distortion, suppression of recombination, low polymorphism levels, and poor viability have also been observed. More recently, the diploid species L. burttii has been identified as a fertile crossing partner of L. japonicus. To assess its qualities in genetic linkage analysis and to enable quantitative trait locus (QTL) mapping for a wider range of traits in Lotus species, we have generated and genotyped a set of 163 Gifu × L. burttii recombinant inbred lines (RILs). By direct comparisons of RIL and F2 population data, we show that L. burttii is a valid alternative to MG-20 as a Gifu mapping partner. In addition, we demonstrate the utility of the Gifu × L. burttii RILs in QTL mapping by identifying an Nfr1-linked QTL for Sinorhizobium fredii nodulation.
Original languageEnglish
JournalD N A Research
Volume19
Issue4
Pages (from-to)317-323
Number of pages7
ISSN1340-2838
DOIs
Publication statusPublished - Aug 2012

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