A robust method of determination of high concentrations of peptides and proteins

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Interdisciplinary Nanoscience Center
In this paper, we pioneer application of a unique method of protein determination by coloring peptide bonds for analysis of a variety of biomolecules with different grades of purity (e.g., oligopeptides, membrane, and glycol proteins). We demonstrated that the calibration curve for all studied molecules is universal and linear within 0.1 to 1.2mg protein content range. The assay thus can be used to analyze peptides without preliminary dilutions and calibration in up to 1g/ml solutions of peptides, which is crucial for many biotechnological processes, such as development of coatings, scaffolds, and biocompatible materials.
Original languageEnglish
JournalAnalytical Biochemistry
Volume395
Issue1
Pages (from-to)111-2
Number of pages2
ISSN0003-2697
DOIs
Publication statusPublished - 1 Dec 2009

    Research areas

  • Calibration, Colorimetry, Copper Sulfate, Indicators and Reagents, Peptides, Proteins

See relations at Aarhus University Citationformats

ID: 17572516