A robust method of determination of high concentrations of peptides and proteins

Pavel A Levashov, Duncan S Sutherland, Flemming Besenbacher, Stepan Shipovskov

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    Abstract

    In this paper, we pioneer application of a unique method of protein determination by coloring peptide bonds for analysis of a variety of biomolecules with different grades of purity (e.g., oligopeptides, membrane, and glycol proteins). We demonstrated that the calibration curve for all studied molecules is universal and linear within 0.1 to 1.2mg protein content range. The assay thus can be used to analyze peptides without preliminary dilutions and calibration in up to 1g/ml solutions of peptides, which is crucial for many biotechnological processes, such as development of coatings, scaffolds, and biocompatible materials.
    Original languageEnglish
    JournalAnalytical Biochemistry
    Volume395
    Issue1
    Pages (from-to)111-2
    Number of pages2
    ISSN0003-2697
    DOIs
    Publication statusPublished - 1 Dec 2009

    Keywords

    • Calibration
    • Colorimetry
    • Copper Sulfate
    • Indicators and Reagents
    • Peptides
    • Proteins

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