A Revised Iron Extraction Protocol for Environmental Samples Rich in Nitrite and Carbonate

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  • Franziska Schaedler, Univ Tubingen, Eberhard Karls University of Tubingen, Ctr Appl Geosci ZAG, Geomicrobiol Grp
  • ,
  • Andreas Kappler
  • Caroline Schmidt, Univ Tubingen, Eberhard Karls University of Tubingen, Ctr Appl Geosci ZAG, Geomicrobiol Grp

Wet-chemical iron extraction is widely applied to quantify the mineral-bound ferriferous fraction of sediments and soils. As previously shown, this method is strongly affected by the composition of the soil/sediment. Samples enriched in mostly microbially produced nitrite require the removal of the nitrite-containing aqueous phase or the replacement of HCl with sulfamic acid (SA) as the extractant. In this study, we show that sedimentary carbonate buffers SA, inhibiting the stabilization of Fe(II) and effective extraction of iron. We therefore provide a revised extraction protocol which allows the preservation of low pH conditions, leading to efficient iron extraction in nitrite-and carbonate-enriched samples.

Original languageEnglish
JournalGeomicrobiology Journal
Volume35
Issue1
Pages (from-to)23-30
Number of pages8
ISSN0149-0451
DOIs
Publication statusPublished - 2018

    Research areas

  • Iron quantification, sediments, soils, COASTAL MARINE-SEDIMENTS, FE(II) OXIDATION, NITRATE REDUCTION, FERROUS IRON, HYDRAZINE REDUCTION, BACTERIA, FERROZINE, DENITRIFICATION, GROUNDWATER, SPECIATION

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