A quencher-free molecular beacon design based on pyrene excimer fluorescence using pyrene-labeled UNA (unlocked nucleic acid)

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

A quencher-free molecular beacon design based on pyrene excimer fluorescence using pyrene-labeled UNA (unlocked nucleic acid). / Karlsen, Kasper Kannegård; Okholm, Anders Hauge; Kjems, Jørgen; Wengel, Jesper.

In: Bioorganic & Medicinal Chemistry, Vol. 21, No. 20, 15.10.2013, p. 6186-6190.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

APA

CBE

Karlsen KK, Okholm AH, Kjems J, Wengel J. 2013. A quencher-free molecular beacon design based on pyrene excimer fluorescence using pyrene-labeled UNA (unlocked nucleic acid). Bioorganic & Medicinal Chemistry. 21(20):6186-6190.

MLA

Karlsen, Kasper Kannegård et al. "A quencher-free molecular beacon design based on pyrene excimer fluorescence using pyrene-labeled UNA (unlocked nucleic acid)". Bioorganic & Medicinal Chemistry. 2013, 21(20). 6186-6190.

Vancouver

Karlsen KK, Okholm AH, Kjems J, Wengel J. A quencher-free molecular beacon design based on pyrene excimer fluorescence using pyrene-labeled UNA (unlocked nucleic acid). Bioorganic & Medicinal Chemistry. 2013 Oct 15;21(20):6186-6190.

Author

Karlsen, Kasper Kannegård ; Okholm, Anders Hauge ; Kjems, Jørgen ; Wengel, Jesper. / A quencher-free molecular beacon design based on pyrene excimer fluorescence using pyrene-labeled UNA (unlocked nucleic acid). In: Bioorganic & Medicinal Chemistry. 2013 ; Vol. 21, No. 20. pp. 6186-6190.

Bibtex

@article{188dccdc31e648c2b9a110aa478c93d0,
title = "A quencher-free molecular beacon design based on pyrene excimer fluorescence using pyrene-labeled UNA (unlocked nucleic acid)",
abstract = "A quencher-free molecular beacon capable of generating pyrene excimer fluorescence has been constructed using strategically positioned pyrene-UNA monomers. Hybridization of a fully complementary RNA target was accompanied by a pyrene excimer emission increase of more than 900%, and detection of RNA in living cells was demonstrated.",
keywords = "Quencher-free molecular beacon, Pyrene excimer, Unlocked nucleic acid (UNA), Nucleic acid detection, Cell imaging",
author = "Karlsen, {Kasper Kanneg{\aa}rd} and Okholm, {Anders Hauge} and J{\o}rgen Kjems and Jesper Wengel",
year = "2013",
month = oct,
day = "15",
language = "English",
volume = "21",
pages = "6186--6190",
journal = "Bioorganic & Medicinal Chemistry",
issn = "0968-0896",
publisher = "Pergamon Press",
number = "20",

}

RIS

TY - JOUR

T1 - A quencher-free molecular beacon design based on pyrene excimer fluorescence using pyrene-labeled UNA (unlocked nucleic acid)

AU - Karlsen, Kasper Kannegård

AU - Okholm, Anders Hauge

AU - Kjems, Jørgen

AU - Wengel, Jesper

PY - 2013/10/15

Y1 - 2013/10/15

N2 - A quencher-free molecular beacon capable of generating pyrene excimer fluorescence has been constructed using strategically positioned pyrene-UNA monomers. Hybridization of a fully complementary RNA target was accompanied by a pyrene excimer emission increase of more than 900%, and detection of RNA in living cells was demonstrated.

AB - A quencher-free molecular beacon capable of generating pyrene excimer fluorescence has been constructed using strategically positioned pyrene-UNA monomers. Hybridization of a fully complementary RNA target was accompanied by a pyrene excimer emission increase of more than 900%, and detection of RNA in living cells was demonstrated.

KW - Quencher-free molecular beacon

KW - Pyrene excimer

KW - Unlocked nucleic acid (UNA)

KW - Nucleic acid detection

KW - Cell imaging

M3 - Journal article

VL - 21

SP - 6186

EP - 6190

JO - Bioorganic & Medicinal Chemistry

JF - Bioorganic & Medicinal Chemistry

SN - 0968-0896

IS - 20

ER -