A quencher-free molecular beacon design based on pyrene excimer fluorescence using pyrene-labeled UNA (unlocked nucleic acid)

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Kasper Kannegård Karlsen, NAC, Institut for Fysik og Kemi, Denmark
  • Anders Hauge Okholm, Denmark
  • Jørgen Kjems
  • Jesper Wengel, Institut for Fysik, Kemi og Farmaci, Denmark
A quencher-free molecular beacon capable of generating pyrene excimer fluorescence has been constructed using strategically positioned pyrene-UNA monomers. Hybridization of a fully complementary RNA target was accompanied by a pyrene excimer emission increase of more than 900%, and detection of RNA in living cells was demonstrated.
Original languageEnglish
JournalBioorganic & Medicinal Chemistry
Pages (from-to)6186-6190
Number of pages6
Publication statusPublished - 15 Oct 2013

    Research areas

  • Quencher-free molecular beacon, Pyrene excimer, Unlocked nucleic acid (UNA), Nucleic acid detection, Cell imaging

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