A Pulmonary Trunk Banding Model of Pressure Overload Induced Right Ventricular Hypertrophy and Failure

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DOI

Right ventricular (RV) failure induced by sustained pressure overload is a major contributor to morbidity and mortality in several cardiopulmonary disorders. Reliable and reproducible animal models of RV failure are therefore warranted in order to investigate disease mechanisms and effects of potential therapeutic strategies. Banding of the pulmonary trunk is a common method to induce isolated RV hypertrophy but in general, previously described models have not succeeded in creating a stable model of RV hypertrophy and failure. We present a rat model of pressure overload induced RV hypertrophy caused by pulmonary trunk banding (PTB) that enables different phenotypes of RV hypertrophy with and without RV failure. We use a modified ligating clip applier to compress a titanium clip around the pulmonary trunk to a pre-set inner diameter. We use different clip diameters to induce different stages of disease progression from mild RV hypertrophy to decompensated RV failure. RV hypertrophy develops consistently in rats subjected to the PTB procedure and depending on the diameter of the applied banding clip, we can accurately reproduce different disease severities ranging from compensated hypertrophy to severe decompensated RV failure with extra-cardiac manifestations. The presented PTB model is a valid and robust model of pressure overload induced RV hypertrophy and failure that has several advantages to other banding models including high reproducibility and the possibility of inducing severe and decompensated RV failure.

Original languageEnglish
Article numbere58050
JournalJournal of Visualized Experiments
Volume2018
Issue141
Number of pages7
ISSN1940-087X
DOIs
Publication statusPublished - 29 Nov 2018

    Research areas

  • Animal model, Congenital heart disease, Issue 141, Medicine, Pulmonary hypertension, Pulmonary trunk banding, Rats, Right ventricular failure, Right ventricular hypertrophy, Congenital Heart Disease, Animal Model, Pulmonary Trunk Banding, RAT MODEL, Right Ventricular Hypertrophy, Pulmonary Hypertension, HYPERTENSION, Right Ventricular Failure

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