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A promising wound dressing based on alginate hydrogels containing vitamin D3 cross-linked by calcium carbonate/d-glucono-δ-lactone

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  • Arian Ehterami, Islamic Azad University
  • ,
  • Majid Salehi, Shahroud University of Medical Sciences
  • ,
  • Saeed Farzamfar, Tehran University of Medical Sciences
  • ,
  • Hadi Samadian, Kermanshah University of Medical Sciences
  • ,
  • Ahmad Vaez, Shiraz University of Medical Sciences
  • ,
  • Hamed Sahrapeyma, Islamic Azad University
  • ,
  • Sadegh Ghorbani

In the present study, we fabricated vitamin D3-loaded alginate hydrogel and assessed its wound healing capability in the animal model. The various concentrations of vitamin D3 were added to the pre-dissolved sodium alginate in deionized water and cross-linked by calcium carbonate in combination with d-glucono-δ-lactone. The microstructure, swelling behavior, weight loss, hemo- and cytocompatibility of the fabricated hydrogels were evaluated. In the last stage, the therapeutic efficacy of the prepared hydrogels was evaluated in the full-thickness dermal wound model. The scanning electron microscopy images showed that the prepared hydrogel was highly porous with the porosity of 89.2 ± 12.5% and contained the interconnected pores. Weight loss assessment showed that the prepared hydrogel is biodegradable with the weight loss percentage of about 89% in 14 days. The results showed that the prepared hydrogels were hemo- and cytocompatible. The animal study results implied that alginate hydrogel/3000 IU vitamin D3 group exhibited the highest wound closure present which was statistically significant than the control group (p < 0.05). Moreover, the histological examinations revealed that hydrogel containing 3000 IU vitamin D3 had the best performance and induced the highest re-epithelialization and granular tissue formation. All in all, this study suggests that alginate hydrogels with 3000 IU vitamin D3 can be exploited as a potential wound dressing in skin tissue engineering.

Original languageEnglish
JournalBiomedical Engineering Letters
Pages (from-to)309-319
Number of pages11
Publication statusPublished - May 2020

    Research areas

  • Alginate hydrogel, Skin, Tissue engineering, Vitamin D, Wound healing

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