A programmable microenvironment for cellular studies via microfluidics-generated double emulsions

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

A programmable microenvironment for cellular studies via microfluidics-generated double emulsions. / Zhang, Ying; Ho, Yi-Ping; Chiu, Ya-Ling; Chan, Hon Fai; Chlebina, Ben; Schuhmann, Tom; You, Lingchong; Leong, Kam W.

In: Biomaterials, Vol. 34, No. 19, 06.2013, p. 4564-72.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Zhang, Y, Ho, Y-P, Chiu, Y-L, Chan, HF, Chlebina, B, Schuhmann, T, You, L & Leong, KW 2013, 'A programmable microenvironment for cellular studies via microfluidics-generated double emulsions', Biomaterials, vol. 34, no. 19, pp. 4564-72. https://doi.org/10.1016/j.biomaterials.2013.03.002

APA

Zhang, Y., Ho, Y-P., Chiu, Y-L., Chan, H. F., Chlebina, B., Schuhmann, T., ... Leong, K. W. (2013). A programmable microenvironment for cellular studies via microfluidics-generated double emulsions. Biomaterials, 34(19), 4564-72. https://doi.org/10.1016/j.biomaterials.2013.03.002

CBE

Zhang Y, Ho Y-P, Chiu Y-L, Chan HF, Chlebina B, Schuhmann T, You L, Leong KW. 2013. A programmable microenvironment for cellular studies via microfluidics-generated double emulsions. Biomaterials. 34(19):4564-72. https://doi.org/10.1016/j.biomaterials.2013.03.002

MLA

Vancouver

Zhang Y, Ho Y-P, Chiu Y-L, Chan HF, Chlebina B, Schuhmann T et al. A programmable microenvironment for cellular studies via microfluidics-generated double emulsions. Biomaterials. 2013 Jun;34(19):4564-72. https://doi.org/10.1016/j.biomaterials.2013.03.002

Author

Zhang, Ying ; Ho, Yi-Ping ; Chiu, Ya-Ling ; Chan, Hon Fai ; Chlebina, Ben ; Schuhmann, Tom ; You, Lingchong ; Leong, Kam W. / A programmable microenvironment for cellular studies via microfluidics-generated double emulsions. In: Biomaterials. 2013 ; Vol. 34, No. 19. pp. 4564-72.

Bibtex

@article{25647312396d4db7ae8ac3b34f0623c6,
title = "A programmable microenvironment for cellular studies via microfluidics-generated double emulsions",
abstract = "High throughput cellular studies require small sample volume to reduce costs and enhance sensitivity. Microfluidics-generated water-in-oil (W/O) single emulsion droplet systems, in particular, provide uniform, well defined and discrete microenvironment for cell culture, screening, and sorting. However, these single emulsion droplets are incapable of continuous supply of nutrient molecule and are not compatible with aqueous phase-based analysis. A solution is to entrap W/O droplets in another aqueous phase, forming water-in-oil-in-water (W/O/W) double emulsions. The external aqueous phase efficiently prevents desiccation and reduces the amount of organic component, and yet retaining the advantages of compartmentalization. The internal environment can also be programmed dynamically without the need of rupturing the droplets. In this study, we explore the potential application of W/O/W double emulsion droplets for cell cultivation, genetic activation and study of more complicated biological events such as bacteria quorum-sensing as an example. This study demonstrates the advantages and potential application of double emulsion for the study of complex biological processes.",
author = "Ying Zhang and Yi-Ping Ho and Ya-Ling Chiu and Chan, {Hon Fai} and Ben Chlebina and Tom Schuhmann and Lingchong You and Leong, {Kam W}",
note = "Copyright {\circledC} 2013 Elsevier Ltd. All rights reserved.",
year = "2013",
month = "6",
doi = "10.1016/j.biomaterials.2013.03.002",
language = "English",
volume = "34",
pages = "4564--72",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "19",

}

RIS

TY - JOUR

T1 - A programmable microenvironment for cellular studies via microfluidics-generated double emulsions

AU - Zhang, Ying

AU - Ho, Yi-Ping

AU - Chiu, Ya-Ling

AU - Chan, Hon Fai

AU - Chlebina, Ben

AU - Schuhmann, Tom

AU - You, Lingchong

AU - Leong, Kam W

N1 - Copyright © 2013 Elsevier Ltd. All rights reserved.

PY - 2013/6

Y1 - 2013/6

N2 - High throughput cellular studies require small sample volume to reduce costs and enhance sensitivity. Microfluidics-generated water-in-oil (W/O) single emulsion droplet systems, in particular, provide uniform, well defined and discrete microenvironment for cell culture, screening, and sorting. However, these single emulsion droplets are incapable of continuous supply of nutrient molecule and are not compatible with aqueous phase-based analysis. A solution is to entrap W/O droplets in another aqueous phase, forming water-in-oil-in-water (W/O/W) double emulsions. The external aqueous phase efficiently prevents desiccation and reduces the amount of organic component, and yet retaining the advantages of compartmentalization. The internal environment can also be programmed dynamically without the need of rupturing the droplets. In this study, we explore the potential application of W/O/W double emulsion droplets for cell cultivation, genetic activation and study of more complicated biological events such as bacteria quorum-sensing as an example. This study demonstrates the advantages and potential application of double emulsion for the study of complex biological processes.

AB - High throughput cellular studies require small sample volume to reduce costs and enhance sensitivity. Microfluidics-generated water-in-oil (W/O) single emulsion droplet systems, in particular, provide uniform, well defined and discrete microenvironment for cell culture, screening, and sorting. However, these single emulsion droplets are incapable of continuous supply of nutrient molecule and are not compatible with aqueous phase-based analysis. A solution is to entrap W/O droplets in another aqueous phase, forming water-in-oil-in-water (W/O/W) double emulsions. The external aqueous phase efficiently prevents desiccation and reduces the amount of organic component, and yet retaining the advantages of compartmentalization. The internal environment can also be programmed dynamically without the need of rupturing the droplets. In this study, we explore the potential application of W/O/W double emulsion droplets for cell cultivation, genetic activation and study of more complicated biological events such as bacteria quorum-sensing as an example. This study demonstrates the advantages and potential application of double emulsion for the study of complex biological processes.

U2 - 10.1016/j.biomaterials.2013.03.002

DO - 10.1016/j.biomaterials.2013.03.002

M3 - Journal article

VL - 34

SP - 4564

EP - 4572

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 19

ER -