A Novel Auto-Focusing Algorithm for Automated Cell Immobilization

Huipeng Zhang; Liying Su; Hongmiao Wei; Yueqing  Yu; Xuping Zhang

doi:10.1109/ICMA.2019.8816338

A Novel Auto-Focusing Algorithm for Automated Cell Immobilization

Huipeng Zhang, Liying Su, Hongmiao Wei, Yueqing Yu, Xuping Zhang

Research output: Contribution to book/anthology/report/proceeding › Article in proceedings › Research › peer-review

Abstract

Automated cell immobilization is a key technology in robotic single cell manipulation. In the existing automation methods of cell immobilization, the microinjection tools (microgripper or micropipette) and the cells need to be manually put into the culture media, and then the microgripper or micropipette is automated to gradually approach the plane in which the center of the cell is located in order to clamp or adsorb the cells to immobilize cell. This paper presents a novel technology that is capable of automatically moving down the gripper into the culture media from the outside air environment, and then precisely clamping a cell in the liquid environment for injection. In the proposed solution, an active window-based auto-focusing algorithm is developed to solve the challenging problem: the image information is lost due to the 'viscous effect' taking place when the gripper jaw touches the water surface and enters the culture media. The proposed algorithm are tested and validated by the immobilization experiments of zebrafish embryos using the in-house develop micro-robotic system. The proposed algorithm in this paper can help firmly immobilize cells, significantly improve the automation of micromanipulation, and reduce the working intensity of the labors.

Original language	English
Title of host publication	Proceedings of 2019 IEEE International Conference on Mechatronics and Automation, ICMA 2019
Number of pages	6
Publisher	IEEE
Publication date	2019
Pages	656-661
Article number	8816338
ISBN (Print)	978-1-7281-1698-3
ISBN (Electronic)	978-1-7281-1699-0
DOIs	https://doi.org/10.1109/ICMA.2019.8816338
Publication status	Published - 2019
Event	2019 IEEE International Conference on Mechatronics and Automation (ICMA) - Tianjin, China Duration: 4 Aug 2019 → 7 Aug 2019

Conference

Conference	2019 IEEE International Conference on Mechatronics and Automation (ICMA)
Country/Territory	China
City	Tianjin
Period	04/08/2019 → 07/08/2019

Keywords

Active window-based auto-focusing algorithm
Automated cell immobilization
Computer vision

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10.1109/ICMA.2019.8816338

https://ieeexplore.ieee.org/document/8816338/authors#authors

Cite this

@inproceedings{f7c50b926979432abc30a05e8dc6fc3d,

title = "A Novel Auto-Focusing Algorithm for Automated Cell Immobilization",

abstract = "Automated cell immobilization is a key technology in robotic single cell manipulation. In the existing automation methods of cell immobilization, the microinjection tools (microgripper or micropipette) and the cells need to be manually put into the culture media, and then the microgripper or micropipette is automated to gradually approach the plane in which the center of the cell is located in order to clamp or adsorb the cells to immobilize cell. This paper presents a novel technology that is capable of automatically moving down the gripper into the culture media from the outside air environment, and then precisely clamping a cell in the liquid environment for injection. In the proposed solution, an active window-based auto-focusing algorithm is developed to solve the challenging problem: the image information is lost due to the 'viscous effect' taking place when the gripper jaw touches the water surface and enters the culture media. The proposed algorithm are tested and validated by the immobilization experiments of zebrafish embryos using the in-house develop micro-robotic system. The proposed algorithm in this paper can help firmly immobilize cells, significantly improve the automation of micromanipulation, and reduce the working intensity of the labors.",

keywords = "Active window-based auto-focusing algorithm, Automated cell immobilization, Computer vision",

author = "Huipeng Zhang and Liying Su and Hongmiao Wei and Yueqing Yu and Xuping Zhang",

year = "2019",

doi = "10.1109/ICMA.2019.8816338",

language = "English",

isbn = "978-1-7281-1698-3 ",

pages = "656--661",

booktitle = "Proceedings of 2019 IEEE International Conference on Mechatronics and Automation, ICMA 2019",

publisher = "IEEE",

note = "2019 IEEE International Conference on Mechatronics and Automation (ICMA) ; Conference date: 04-08-2019 Through 07-08-2019",

}

Zhang, H, Su, L, Wei, H, Yu, Y & Zhang, X 2019, A Novel Auto-Focusing Algorithm for Automated Cell Immobilization. in Proceedings of 2019 IEEE International Conference on Mechatronics and Automation, ICMA 2019., 8816338, IEEE, pp. 656-661, 2019 IEEE International Conference on Mechatronics and Automation (ICMA) , Tianjin, China, 04/08/2019. https://doi.org/10.1109/ICMA.2019.8816338

TY - GEN

T1 - A Novel Auto-Focusing Algorithm for Automated Cell Immobilization

AU - Zhang, Huipeng

AU - Su, Liying

AU - Wei, Hongmiao

AU - Yu, Yueqing

AU - Zhang, Xuping

PY - 2019

Y1 - 2019

N2 - Automated cell immobilization is a key technology in robotic single cell manipulation. In the existing automation methods of cell immobilization, the microinjection tools (microgripper or micropipette) and the cells need to be manually put into the culture media, and then the microgripper or micropipette is automated to gradually approach the plane in which the center of the cell is located in order to clamp or adsorb the cells to immobilize cell. This paper presents a novel technology that is capable of automatically moving down the gripper into the culture media from the outside air environment, and then precisely clamping a cell in the liquid environment for injection. In the proposed solution, an active window-based auto-focusing algorithm is developed to solve the challenging problem: the image information is lost due to the 'viscous effect' taking place when the gripper jaw touches the water surface and enters the culture media. The proposed algorithm are tested and validated by the immobilization experiments of zebrafish embryos using the in-house develop micro-robotic system. The proposed algorithm in this paper can help firmly immobilize cells, significantly improve the automation of micromanipulation, and reduce the working intensity of the labors.

AB - Automated cell immobilization is a key technology in robotic single cell manipulation. In the existing automation methods of cell immobilization, the microinjection tools (microgripper or micropipette) and the cells need to be manually put into the culture media, and then the microgripper or micropipette is automated to gradually approach the plane in which the center of the cell is located in order to clamp or adsorb the cells to immobilize cell. This paper presents a novel technology that is capable of automatically moving down the gripper into the culture media from the outside air environment, and then precisely clamping a cell in the liquid environment for injection. In the proposed solution, an active window-based auto-focusing algorithm is developed to solve the challenging problem: the image information is lost due to the 'viscous effect' taking place when the gripper jaw touches the water surface and enters the culture media. The proposed algorithm are tested and validated by the immobilization experiments of zebrafish embryos using the in-house develop micro-robotic system. The proposed algorithm in this paper can help firmly immobilize cells, significantly improve the automation of micromanipulation, and reduce the working intensity of the labors.

KW - Active window-based auto-focusing algorithm

KW - Automated cell immobilization

KW - Computer vision

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U2 - 10.1109/ICMA.2019.8816338

DO - 10.1109/ICMA.2019.8816338

M3 - Article in proceedings

SN - 978-1-7281-1698-3

SP - 656

EP - 661

BT - Proceedings of 2019 IEEE International Conference on Mechatronics and Automation, ICMA 2019

PB - IEEE

T2 - 2019 IEEE International Conference on Mechatronics and Automation (ICMA)

Y2 - 4 August 2019 through 7 August 2019

ER -

A Novel Auto-Focusing Algorithm for Automated Cell Immobilization

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