A new plant-based drug delivery platform based on alkyl polyglucosides and β-sitosterol nanovesicles for topical delivery

Marta Alcaina-Hernando; Ivana Malvacio; Ilaria Ferraboschi; Cristián Huck-Iriart; Annalisa Bianchera; Santi Sala; Jan Skov Pedersen; Lidia Ferrer-Tasies; Silvia Pescina; Cristina Sissa; Nora Ventosa; Alba Córdoba

doi:10.1016/j.apmt.2024.102467

A new plant-based drug delivery platform based on alkyl polyglucosides and β-sitosterol nanovesicles for topical delivery

Marta Alcaina-Hernando, Ivana Malvacio, Ilaria Ferraboschi, Cristián Huck-Iriart, Annalisa Bianchera, Santi Sala, Jan Skov Pedersen, Lidia Ferrer-Tasies, Silvia Pescina, Cristina Sissa, Nora Ventosa^*, Alba Córdoba^*

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Abstract

The finding of new vesicular systems is a challenging process that depends on different factors such as the components used, the interactions between them or the dispersant media. Our objective was to develop a new vesicular delivery system formed by self-assembly of β-Sitosterol (Sit), Lauryl Glucoside (LGL) and Lauryl Glucose Carboxylate (LGC) molecules, all plant-based, biodegradable and biocompatible components. Nanovesicles (NVs) with different molar ratios of Sit, LGL and LGC were prepared using a single step method named DELOS, and characterized by dynamic light scattering, cryo-electron microscopy and small-angle X-ray scattering. Antioxidant compound α-tocopherol (TCP) was integrated in the NVs showing their potential to nanoformulate hydrophobic payloads. Finally, in vitro biocompatibility assays with reconstructed human epidermis and ex vivo skin retention studies using multiphoton microscopy and NVs labelled with Nile Red (NR) were carried out. As a result of this work, a new platform of NVs has been obtained by the self-assembly of Sit, LGL and LGC, obtaining vesicular systems with tunable physicochemical properties in terms of size (130 – 220 nm), surface charge ((-70) – (-40) mV) and lamellarity (unilamellar and multilamellar vesicles), when the carbon chain of the alkyl polyglucoside was >12. The vesicles could efficiently integrate TCP, proving their potential role as delivery systems and maintaining its antioxidant activity after loading. Finally, they also showed biocompatibility with the skin and improved the permeability of the poorly water-soluble molecule NR in terms of time and depth through the epidermis. Overall, the results of this work point to the successful development of an attractive platform based on stable and homogeneous nanovesicles composed of plant-derived ingredients for topical delivery.

Original language	English
Article number	102467
Journal	Applied Materials Today
Volume	41
Number of pages	13
ISSN	2352-9407
DOIs	https://doi.org/10.1016/j.apmt.2024.102467
Publication status	Published - Dec 2024

Keywords

Alkypolyglucosides
Biocompatible
Delivery system
Nanovesicle
Phytosterols
Plant based
Topical delivery

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10.1016/j.apmt.2024.102467Licence: CC BY-NC

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Alcaina-Hernando, M., Malvacio, I., Ferraboschi, I., Huck-Iriart, C., Bianchera, A., Sala, S., Pedersen, J. S., Ferrer-Tasies, L., Pescina, S., Sissa, C., Ventosa, N., & Córdoba, A. (2024). A new plant-based drug delivery platform based on alkyl polyglucosides and β-sitosterol nanovesicles for topical delivery. Applied Materials Today, 41, Article 102467. https://doi.org/10.1016/j.apmt.2024.102467

@article{f58a535bcb0149c8adf0c0213646d2a8,

title = "A new plant-based drug delivery platform based on alkyl polyglucosides and β-sitosterol nanovesicles for topical delivery",

abstract = "The finding of new vesicular systems is a challenging process that depends on different factors such as the components used, the interactions between them or the dispersant media. Our objective was to develop a new vesicular delivery system formed by self-assembly of β-Sitosterol (Sit), Lauryl Glucoside (LGL) and Lauryl Glucose Carboxylate (LGC) molecules, all plant-based, biodegradable and biocompatible components. Nanovesicles (NVs) with different molar ratios of Sit, LGL and LGC were prepared using a single step method named DELOS, and characterized by dynamic light scattering, cryo-electron microscopy and small-angle X-ray scattering. Antioxidant compound α-tocopherol (TCP) was integrated in the NVs showing their potential to nanoformulate hydrophobic payloads. Finally, in vitro biocompatibility assays with reconstructed human epidermis and ex vivo skin retention studies using multiphoton microscopy and NVs labelled with Nile Red (NR) were carried out. As a result of this work, a new platform of NVs has been obtained by the self-assembly of Sit, LGL and LGC, obtaining vesicular systems with tunable physicochemical properties in terms of size (130 – 220 nm), surface charge ((-70) – (-40) mV) and lamellarity (unilamellar and multilamellar vesicles), when the carbon chain of the alkyl polyglucoside was >12. The vesicles could efficiently integrate TCP, proving their potential role as delivery systems and maintaining its antioxidant activity after loading. Finally, they also showed biocompatibility with the skin and improved the permeability of the poorly water-soluble molecule NR in terms of time and depth through the epidermis. Overall, the results of this work point to the successful development of an attractive platform based on stable and homogeneous nanovesicles composed of plant-derived ingredients for topical delivery.",

keywords = "Alkypolyglucosides, Biocompatible, Delivery system, Nanovesicle, Phytosterols, Plant based, Topical delivery",

author = "Marta Alcaina-Hernando and Ivana Malvacio and Ilaria Ferraboschi and Cristi{\'a}n Huck-Iriart and Annalisa Bianchera and Santi Sala and Pedersen, {Jan Skov} and Lidia Ferrer-Tasies and Silvia Pescina and Cristina Sissa and Nora Ventosa and Alba C{\'o}rdoba",

note = "Publisher Copyright: {\textcopyright} 2024",

year = "2024",

month = dec,

doi = "10.1016/j.apmt.2024.102467",

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volume = "41",

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Alcaina-Hernando, M, Malvacio, I, Ferraboschi, I, Huck-Iriart, C, Bianchera, A, Sala, S, Pedersen, JS, Ferrer-Tasies, L, Pescina, S, Sissa, C, Ventosa, N & Córdoba, A 2024, 'A new plant-based drug delivery platform based on alkyl polyglucosides and β-sitosterol nanovesicles for topical delivery', Applied Materials Today, vol. 41, 102467. https://doi.org/10.1016/j.apmt.2024.102467

A new plant-based drug delivery platform based on alkyl polyglucosides and β-sitosterol nanovesicles for topical delivery. / Alcaina-Hernando, Marta; Malvacio, Ivana; Ferraboschi, Ilaria et al.
In: Applied Materials Today, Vol. 41, 102467, 12.2024.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - A new plant-based drug delivery platform based on alkyl polyglucosides and β-sitosterol nanovesicles for topical delivery

AU - Alcaina-Hernando, Marta

AU - Malvacio, Ivana

AU - Ferraboschi, Ilaria

AU - Huck-Iriart, Cristián

AU - Bianchera, Annalisa

AU - Sala, Santi

AU - Pedersen, Jan Skov

AU - Ferrer-Tasies, Lidia

AU - Pescina, Silvia

AU - Sissa, Cristina

AU - Ventosa, Nora

AU - Córdoba, Alba

PY - 2024/12

Y1 - 2024/12

N2 - The finding of new vesicular systems is a challenging process that depends on different factors such as the components used, the interactions between them or the dispersant media. Our objective was to develop a new vesicular delivery system formed by self-assembly of β-Sitosterol (Sit), Lauryl Glucoside (LGL) and Lauryl Glucose Carboxylate (LGC) molecules, all plant-based, biodegradable and biocompatible components. Nanovesicles (NVs) with different molar ratios of Sit, LGL and LGC were prepared using a single step method named DELOS, and characterized by dynamic light scattering, cryo-electron microscopy and small-angle X-ray scattering. Antioxidant compound α-tocopherol (TCP) was integrated in the NVs showing their potential to nanoformulate hydrophobic payloads. Finally, in vitro biocompatibility assays with reconstructed human epidermis and ex vivo skin retention studies using multiphoton microscopy and NVs labelled with Nile Red (NR) were carried out. As a result of this work, a new platform of NVs has been obtained by the self-assembly of Sit, LGL and LGC, obtaining vesicular systems with tunable physicochemical properties in terms of size (130 – 220 nm), surface charge ((-70) – (-40) mV) and lamellarity (unilamellar and multilamellar vesicles), when the carbon chain of the alkyl polyglucoside was >12. The vesicles could efficiently integrate TCP, proving their potential role as delivery systems and maintaining its antioxidant activity after loading. Finally, they also showed biocompatibility with the skin and improved the permeability of the poorly water-soluble molecule NR in terms of time and depth through the epidermis. Overall, the results of this work point to the successful development of an attractive platform based on stable and homogeneous nanovesicles composed of plant-derived ingredients for topical delivery.

AB - The finding of new vesicular systems is a challenging process that depends on different factors such as the components used, the interactions between them or the dispersant media. Our objective was to develop a new vesicular delivery system formed by self-assembly of β-Sitosterol (Sit), Lauryl Glucoside (LGL) and Lauryl Glucose Carboxylate (LGC) molecules, all plant-based, biodegradable and biocompatible components. Nanovesicles (NVs) with different molar ratios of Sit, LGL and LGC were prepared using a single step method named DELOS, and characterized by dynamic light scattering, cryo-electron microscopy and small-angle X-ray scattering. Antioxidant compound α-tocopherol (TCP) was integrated in the NVs showing their potential to nanoformulate hydrophobic payloads. Finally, in vitro biocompatibility assays with reconstructed human epidermis and ex vivo skin retention studies using multiphoton microscopy and NVs labelled with Nile Red (NR) were carried out. As a result of this work, a new platform of NVs has been obtained by the self-assembly of Sit, LGL and LGC, obtaining vesicular systems with tunable physicochemical properties in terms of size (130 – 220 nm), surface charge ((-70) – (-40) mV) and lamellarity (unilamellar and multilamellar vesicles), when the carbon chain of the alkyl polyglucoside was >12. The vesicles could efficiently integrate TCP, proving their potential role as delivery systems and maintaining its antioxidant activity after loading. Finally, they also showed biocompatibility with the skin and improved the permeability of the poorly water-soluble molecule NR in terms of time and depth through the epidermis. Overall, the results of this work point to the successful development of an attractive platform based on stable and homogeneous nanovesicles composed of plant-derived ingredients for topical delivery.

KW - Alkypolyglucosides

KW - Biocompatible

KW - Delivery system

KW - Nanovesicle

KW - Phytosterols

KW - Plant based

KW - Topical delivery

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U2 - 10.1016/j.apmt.2024.102467

DO - 10.1016/j.apmt.2024.102467

M3 - Journal article

AN - SCOPUS:85206502782

SN - 2352-9407

VL - 41

JO - Applied Materials Today

JF - Applied Materials Today

M1 - 102467

ER -

A new plant-based drug delivery platform based on alkyl polyglucosides and β-sitosterol nanovesicles for topical delivery

Abstract

Keywords

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