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A New High-Throughput Screening Method for Phages: Enabling Crude Isolation and Fast Identification of Diverse Phages with Therapeutic Potential

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A New High-Throughput Screening Method for Phages : Enabling Crude Isolation and Fast Identification of Diverse Phages with Therapeutic Potential. / Olsen, Nikoline S.; Hendriksen, Niels Bohse; Hansen, Lars Hestbjerg et al.

In: PHAGE: Therapy, Applications and Research, Vol. 1, No. 3, 09.2020, p. 137-148.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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Olsen, Nikoline S. ; Hendriksen, Niels Bohse ; Hansen, Lars Hestbjerg et al. / A New High-Throughput Screening Method for Phages : Enabling Crude Isolation and Fast Identification of Diverse Phages with Therapeutic Potential. In: PHAGE: Therapy, Applications and Research. 2020 ; Vol. 1, No. 3. pp. 137-148.

Bibtex

@article{8fac841ebab14f51b2d0e954dd6c8fbb,
title = "A New High-Throughput Screening Method for Phages: Enabling Crude Isolation and Fast Identification of Diverse Phages with Therapeutic Potential",
abstract = "Bacteriophage therapy and application of phages for biocontrol necessitate acquisition of suitable phages. The exclusivity of phage–host relations and the risk of phage resistance instigate a need to rapidly isolate and characterize novel phages and continually build sizeable phage libraries. Current methods for phage isolation are both laborious and time consuming, suitable for the isolation of a limited number of phages. The high-throughput screening method for phages upscales and organizes enrichment of phages for fast isolation and identification of potentially hundreds of distinct phages against single hosts. This enables screening of hundreds of samples, in multiple simultaneous setups with varying parameters, increasing the likelihood of isolating multiple distinct phages specific for the given conditions. The efficiency of the method is emphasized by our screening of 200 environmental samples, resulting in the identification of an abundance of unique phage species virulent to Escherichia coli, Salmonella enterica, Enterococcus faecalis, and Pseudomonas aeruginosa.",
keywords = "Bacteriophage, phage isolation, phage therapy, high-throughput, wastewater",
author = "Olsen, {Nikoline S.} and Hendriksen, {Niels Bohse} and Hansen, {Lars Hestbjerg} and Witold Kot",
year = "2020",
month = sep,
doi = "10.1089/phage.2020.0016",
language = "English",
volume = "1",
pages = "137--148",
journal = "PHAGE: Therapy, Applications and Research",
issn = "2641-6530",
publisher = "Mary Ann Liebert",
number = "3",

}

RIS

TY - JOUR

T1 - A New High-Throughput Screening Method for Phages

T2 - Enabling Crude Isolation and Fast Identification of Diverse Phages with Therapeutic Potential

AU - Olsen, Nikoline S.

AU - Hendriksen, Niels Bohse

AU - Hansen, Lars Hestbjerg

AU - Kot, Witold

PY - 2020/9

Y1 - 2020/9

N2 - Bacteriophage therapy and application of phages for biocontrol necessitate acquisition of suitable phages. The exclusivity of phage–host relations and the risk of phage resistance instigate a need to rapidly isolate and characterize novel phages and continually build sizeable phage libraries. Current methods for phage isolation are both laborious and time consuming, suitable for the isolation of a limited number of phages. The high-throughput screening method for phages upscales and organizes enrichment of phages for fast isolation and identification of potentially hundreds of distinct phages against single hosts. This enables screening of hundreds of samples, in multiple simultaneous setups with varying parameters, increasing the likelihood of isolating multiple distinct phages specific for the given conditions. The efficiency of the method is emphasized by our screening of 200 environmental samples, resulting in the identification of an abundance of unique phage species virulent to Escherichia coli, Salmonella enterica, Enterococcus faecalis, and Pseudomonas aeruginosa.

AB - Bacteriophage therapy and application of phages for biocontrol necessitate acquisition of suitable phages. The exclusivity of phage–host relations and the risk of phage resistance instigate a need to rapidly isolate and characterize novel phages and continually build sizeable phage libraries. Current methods for phage isolation are both laborious and time consuming, suitable for the isolation of a limited number of phages. The high-throughput screening method for phages upscales and organizes enrichment of phages for fast isolation and identification of potentially hundreds of distinct phages against single hosts. This enables screening of hundreds of samples, in multiple simultaneous setups with varying parameters, increasing the likelihood of isolating multiple distinct phages specific for the given conditions. The efficiency of the method is emphasized by our screening of 200 environmental samples, resulting in the identification of an abundance of unique phage species virulent to Escherichia coli, Salmonella enterica, Enterococcus faecalis, and Pseudomonas aeruginosa.

KW - Bacteriophage

KW - phage isolation

KW - phage therapy

KW - high-throughput

KW - wastewater

U2 - 10.1089/phage.2020.0016

DO - 10.1089/phage.2020.0016

M3 - Journal article

VL - 1

SP - 137

EP - 148

JO - PHAGE: Therapy, Applications and Research

JF - PHAGE: Therapy, Applications and Research

SN - 2641-6530

IS - 3

ER -