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A New High-Throughput Screening Method for Phages: Enabling Crude Isolation and Fast Identification of Diverse Phages with Therapeutic Potential

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  • Nikoline S. Olsen
  • ,
  • Niels Bohse Hendriksen
  • Lars Hestbjerg Hansen, University of Copenhagen
  • ,
  • Witold Kot, University of Copenhagen
Bacteriophage therapy and application of phages for biocontrol necessitate acquisition of suitable phages. The exclusivity of phage–host relations and the risk of phage resistance instigate a need to rapidly isolate and characterize novel phages and continually build sizeable phage libraries. Current methods for phage isolation are both laborious and time consuming, suitable for the isolation of a limited number of phages. The high-throughput screening method for phages upscales and organizes enrichment of phages for fast isolation and identification of potentially hundreds of distinct phages against single hosts. This enables screening of hundreds of samples, in multiple simultaneous setups with varying parameters, increasing the likelihood of isolating multiple distinct phages specific for the given conditions. The efficiency of the method is emphasized by our screening of 200 environmental samples, resulting in the identification of an abundance of unique phage species virulent to Escherichia coli, Salmonella enterica, Enterococcus faecalis, and Pseudomonas aeruginosa.
Original languageEnglish
JournalPHAGE: Therapy, Applications and Research
Pages (from-to)137-148
Number of pages12
Publication statusPublished - Sep 2020

    Research areas

  • Bacteriophage, phage isolation, phage therapy, high-throughput, wastewater

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