A murine DC-SIGN homologue contributes to early host defense against Mycobacterium tuberculosis

Antoine Tanne; Bo Ma; Frédéric Boudou; Ludovic Tailleux; Hélène Botella; Edgar Badell; Florence Levillain; Maureen E Taylor; Kurt Drickamer; Jérome Nigou; Karen M Dobos; Germain Puzo; Dietmar Vestweber; Martin K Wild; Marie Marcinko; Peter Sobieszczuk; Lauren Stewart; Daniel Lebus; Brigitte Gicquel; Olivier Neyrolles

doi:10.1084/jem.20090188

A murine DC-SIGN homologue contributes to early host defense against Mycobacterium tuberculosis

Antoine Tanne, Bo Ma, Frédéric Boudou, Ludovic Tailleux, Hélène Botella, Edgar Badell, Florence Levillain, Maureen E Taylor, Kurt Drickamer, Jérome Nigou, Karen M Dobos, Germain Puzo, Dietmar Vestweber, Martin K Wild, Marie Marcinko, Peter Sobieszczuk, Lauren Stewart, Daniel Lebus, Brigitte Gicquel, Olivier Neyrolles

Department of Clinical Medicine - Center for Music In the Brain

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Abstract

The C-type lectin dendritic cell-specific intercellular adhesion molecule-3 grabbing nonintegrin (DC-SIGN) mediates the innate immune recognition of microbial carbohydrates. We investigated the function of this molecule in the host response to pathogens in vivo, by generating mouse lines lacking the DC-SIGN homologues SIGNR1, SIGNR3, and SIGNR5. Resistance to Mycobacterium tuberculosis was impaired only in SIGNR3-deficient animals. SIGNR3 was expressed in lung phagocytes during infection, and interacted with M. tuberculosis bacilli and mycobacterial surface glycoconjugates to induce secretion of critical host defense inflammatory cytokines, including tumor necrosis factor (TNF). SIGNR3 signaling was dependent on an intracellular tyrosine-based motif and the tyrosine kinase Syk. Thus, the mouse DC-SIGN homologue SIGNR3 makes a unique contribution to protection of the host against a pulmonary bacterial pathogen.

Original language	English
Journal	Journal of Experimental Medicine
Volume	206
Issue	10
Pages (from-to)	2205-20
Number of pages	16
ISSN	0022-1007
DOIs	https://doi.org/10.1084/jem.20090188
Publication status	Published - 28 Sept 2009

Access to Document

10.1084/jem.20090188

Cite this

Tanne, A., Ma, B., Boudou, F., Tailleux, L., Botella, H., Badell, E., Levillain, F., Taylor, M. E., Drickamer, K., Nigou, J., Dobos, K. M., Puzo, G., Vestweber, D., Wild, M. K., Marcinko, M., Sobieszczuk, P., Stewart, L., Lebus, D., Gicquel, B., & Neyrolles, O. (2009). A murine DC-SIGN homologue contributes to early host defense against Mycobacterium tuberculosis. Journal of Experimental Medicine, 206(10), 2205-20. https://doi.org/10.1084/jem.20090188

@article{d146731b4acb49e5b7ee25f41fd97425,

title = "A murine DC-SIGN homologue contributes to early host defense against Mycobacterium tuberculosis",

abstract = "The C-type lectin dendritic cell-specific intercellular adhesion molecule-3 grabbing nonintegrin (DC-SIGN) mediates the innate immune recognition of microbial carbohydrates. We investigated the function of this molecule in the host response to pathogens in vivo, by generating mouse lines lacking the DC-SIGN homologues SIGNR1, SIGNR3, and SIGNR5. Resistance to Mycobacterium tuberculosis was impaired only in SIGNR3-deficient animals. SIGNR3 was expressed in lung phagocytes during infection, and interacted with M. tuberculosis bacilli and mycobacterial surface glycoconjugates to induce secretion of critical host defense inflammatory cytokines, including tumor necrosis factor (TNF). SIGNR3 signaling was dependent on an intracellular tyrosine-based motif and the tyrosine kinase Syk. Thus, the mouse DC-SIGN homologue SIGNR3 makes a unique contribution to protection of the host against a pulmonary bacterial pathogen.",

keywords = "Animals, Antigens, CD, Cell Adhesion Molecules, Extracellular Signal-Regulated MAP Kinases, Female, Glycoconjugates, Interleukin-6, Lectins, C-Type, Lipopolysaccharides, Lung, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, NF-kappa B, Proto-Oncogene Proteins c-raf, Receptors, Cell Surface, Signal Transduction, Toll-Like Receptor 2, Tuberculosis, Tumor Necrosis Factor-alpha",

author = "Antoine Tanne and Bo Ma and Fr{\'e}d{\'e}ric Boudou and Ludovic Tailleux and H{\'e}l{\`e}ne Botella and Edgar Badell and Florence Levillain and Taylor, {Maureen E} and Kurt Drickamer and J{\'e}rome Nigou and Dobos, {Karen M} and Germain Puzo and Dietmar Vestweber and Wild, {Martin K} and Marie Marcinko and Peter Sobieszczuk and Lauren Stewart and Daniel Lebus and Brigitte Gicquel and Olivier Neyrolles",

year = "2009",

month = sep,

day = "28",

doi = "10.1084/jem.20090188",

language = "English",

volume = "206",

pages = "2205--20",

journal = "Journal of Experimental Medicine",

issn = "0022-1007",

publisher = "Rockefeller University Press",

number = "10",

}

Tanne, A, Ma, B, Boudou, F, Tailleux, L, Botella, H, Badell, E, Levillain, F, Taylor, ME, Drickamer, K, Nigou, J, Dobos, KM, Puzo, G, Vestweber, D, Wild, MK, Marcinko, M, Sobieszczuk, P, Stewart, L, Lebus, D, Gicquel, B & Neyrolles, O 2009, 'A murine DC-SIGN homologue contributes to early host defense against Mycobacterium tuberculosis', Journal of Experimental Medicine, vol. 206, no. 10, pp. 2205-20. https://doi.org/10.1084/jem.20090188

A murine DC-SIGN homologue contributes to early host defense against Mycobacterium tuberculosis. / Tanne, Antoine; Ma, Bo; Boudou, Frédéric et al.
In: Journal of Experimental Medicine, Vol. 206, No. 10, 28.09.2009, p. 2205-20.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - A murine DC-SIGN homologue contributes to early host defense against Mycobacterium tuberculosis

AU - Tanne, Antoine

AU - Ma, Bo

AU - Boudou, Frédéric

AU - Tailleux, Ludovic

AU - Botella, Hélène

AU - Badell, Edgar

AU - Levillain, Florence

AU - Taylor, Maureen E

AU - Drickamer, Kurt

AU - Nigou, Jérome

AU - Dobos, Karen M

AU - Puzo, Germain

AU - Vestweber, Dietmar

AU - Wild, Martin K

AU - Marcinko, Marie

AU - Sobieszczuk, Peter

AU - Stewart, Lauren

AU - Lebus, Daniel

AU - Gicquel, Brigitte

AU - Neyrolles, Olivier

PY - 2009/9/28

Y1 - 2009/9/28

N2 - The C-type lectin dendritic cell-specific intercellular adhesion molecule-3 grabbing nonintegrin (DC-SIGN) mediates the innate immune recognition of microbial carbohydrates. We investigated the function of this molecule in the host response to pathogens in vivo, by generating mouse lines lacking the DC-SIGN homologues SIGNR1, SIGNR3, and SIGNR5. Resistance to Mycobacterium tuberculosis was impaired only in SIGNR3-deficient animals. SIGNR3 was expressed in lung phagocytes during infection, and interacted with M. tuberculosis bacilli and mycobacterial surface glycoconjugates to induce secretion of critical host defense inflammatory cytokines, including tumor necrosis factor (TNF). SIGNR3 signaling was dependent on an intracellular tyrosine-based motif and the tyrosine kinase Syk. Thus, the mouse DC-SIGN homologue SIGNR3 makes a unique contribution to protection of the host against a pulmonary bacterial pathogen.

AB - The C-type lectin dendritic cell-specific intercellular adhesion molecule-3 grabbing nonintegrin (DC-SIGN) mediates the innate immune recognition of microbial carbohydrates. We investigated the function of this molecule in the host response to pathogens in vivo, by generating mouse lines lacking the DC-SIGN homologues SIGNR1, SIGNR3, and SIGNR5. Resistance to Mycobacterium tuberculosis was impaired only in SIGNR3-deficient animals. SIGNR3 was expressed in lung phagocytes during infection, and interacted with M. tuberculosis bacilli and mycobacterial surface glycoconjugates to induce secretion of critical host defense inflammatory cytokines, including tumor necrosis factor (TNF). SIGNR3 signaling was dependent on an intracellular tyrosine-based motif and the tyrosine kinase Syk. Thus, the mouse DC-SIGN homologue SIGNR3 makes a unique contribution to protection of the host against a pulmonary bacterial pathogen.

KW - Animals

KW - Antigens, CD

KW - Cell Adhesion Molecules

KW - Extracellular Signal-Regulated MAP Kinases

KW - Female

KW - Glycoconjugates

KW - Interleukin-6

KW - Lectins, C-Type

KW - Lipopolysaccharides

KW - Lung

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Knockout

KW - NF-kappa B

KW - Proto-Oncogene Proteins c-raf

KW - Receptors, Cell Surface

KW - Signal Transduction

KW - Toll-Like Receptor 2

KW - Tuberculosis

KW - Tumor Necrosis Factor-alpha

U2 - 10.1084/jem.20090188

DO - 10.1084/jem.20090188

M3 - Journal article

C2 - 19770268

SN - 0022-1007

VL - 206

SP - 2205

EP - 2220

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

IS - 10

ER -