A Multi-Faceted Binding Assessment of Aptamers Targeting the SARS-CoV-2 Spike Protein

Laia Civit; Nima Moradzadeh; Anna Jonczyk; Patrick Neckermann; Benedikt Asbach; David Peterhoff; Ralf Wagner; Michael Famulok; Günter Mayer; Jørgen Kjems; Julián Valero

doi:10.3390/ijms25094642

A Multi-Faceted Binding Assessment of Aptamers Targeting the SARS-CoV-2 Spike Protein

Laia Civit, Nima Moradzadeh, Anna Jonczyk, Patrick Neckermann, Benedikt Asbach, David Peterhoff, Ralf Wagner, Michael Famulok, Günter Mayer^*, Jørgen Kjems, Julián Valero^*

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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Abstract

The COVID-19 pandemic has underscored the critical need for the advancement of diagnostic and therapeutic platforms. These platforms rely on the rapid development of molecular binders that should facilitate surveillance and swift intervention against viral infections. In this study, we have evaluated by three independent research groups the binding characteristics of various published RNA and DNA aptamers targeting the spike protein of the SARS-CoV-2 virus. For this comparative analysis, we have employed different techniques such as biolayer interferometry (BLI), enzyme-linked oligonucleotide assay (ELONA), and flow cytometry. Our data show discrepancies in the reported specificity and affinity among several of the published aptamers and underline the importance of standardized methods, the impact of biophysical techniques, and the controls used for aptamer characterization. We expect our results to contribute to the selection and application of suitable aptamers for the detection of SARS-CoV-2.

Original language	English
Article number	4642
Journal	International Journal of Molecular Sciences
Volume	25
Issue	9
Number of pages	13
ISSN	1661-6596
DOIs	https://doi.org/10.3390/ijms25094642
Publication status	Published - May 2024

Keywords

aptamers
comparative analysis
COVID-19
SARS-CoV-2
spike protein

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title = "A Multi-Faceted Binding Assessment of Aptamers Targeting the SARS-CoV-2 Spike Protein",

abstract = "The COVID-19 pandemic has underscored the critical need for the advancement of diagnostic and therapeutic platforms. These platforms rely on the rapid development of molecular binders that should facilitate surveillance and swift intervention against viral infections. In this study, we have evaluated by three independent research groups the binding characteristics of various published RNA and DNA aptamers targeting the spike protein of the SARS-CoV-2 virus. For this comparative analysis, we have employed different techniques such as biolayer interferometry (BLI), enzyme-linked oligonucleotide assay (ELONA), and flow cytometry. Our data show discrepancies in the reported specificity and affinity among several of the published aptamers and underline the importance of standardized methods, the impact of biophysical techniques, and the controls used for aptamer characterization. We expect our results to contribute to the selection and application of suitable aptamers for the detection of SARS-CoV-2.",

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doi = "10.3390/ijms25094642",

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A Multi-Faceted Binding Assessment of Aptamers Targeting the SARS-CoV-2 Spike Protein. / Civit, Laia; Moradzadeh, Nima; Jonczyk, Anna et al.
In: International Journal of Molecular Sciences , Vol. 25, No. 9, 4642, 05.2024.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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T1 - A Multi-Faceted Binding Assessment of Aptamers Targeting the SARS-CoV-2 Spike Protein

AU - Civit, Laia

AU - Moradzadeh, Nima

AU - Jonczyk, Anna

AU - Neckermann, Patrick

AU - Asbach, Benedikt

AU - Peterhoff, David

AU - Wagner, Ralf

AU - Famulok, Michael

AU - Mayer, Günter

AU - Kjems, Jørgen

AU - Valero, Julián

PY - 2024/5

Y1 - 2024/5

N2 - The COVID-19 pandemic has underscored the critical need for the advancement of diagnostic and therapeutic platforms. These platforms rely on the rapid development of molecular binders that should facilitate surveillance and swift intervention against viral infections. In this study, we have evaluated by three independent research groups the binding characteristics of various published RNA and DNA aptamers targeting the spike protein of the SARS-CoV-2 virus. For this comparative analysis, we have employed different techniques such as biolayer interferometry (BLI), enzyme-linked oligonucleotide assay (ELONA), and flow cytometry. Our data show discrepancies in the reported specificity and affinity among several of the published aptamers and underline the importance of standardized methods, the impact of biophysical techniques, and the controls used for aptamer characterization. We expect our results to contribute to the selection and application of suitable aptamers for the detection of SARS-CoV-2.

AB - The COVID-19 pandemic has underscored the critical need for the advancement of diagnostic and therapeutic platforms. These platforms rely on the rapid development of molecular binders that should facilitate surveillance and swift intervention against viral infections. In this study, we have evaluated by three independent research groups the binding characteristics of various published RNA and DNA aptamers targeting the spike protein of the SARS-CoV-2 virus. For this comparative analysis, we have employed different techniques such as biolayer interferometry (BLI), enzyme-linked oligonucleotide assay (ELONA), and flow cytometry. Our data show discrepancies in the reported specificity and affinity among several of the published aptamers and underline the importance of standardized methods, the impact of biophysical techniques, and the controls used for aptamer characterization. We expect our results to contribute to the selection and application of suitable aptamers for the detection of SARS-CoV-2.

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KW - comparative analysis

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KW - spike protein

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A Multi-Faceted Binding Assessment of Aptamers Targeting the SARS-CoV-2 Spike Protein

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