A microRNA-129-5p/Rbfox crosstalk coordinates homeostatic downscaling of excitatory synapses

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DOI

  • Marek Rajman, Philipps-Universität Marburg
  • ,
  • Franziska Metge, HIT, University Hospital, Heidelberg
  • ,
  • Roberto Fiore, Philipps-Universität Marburg
  • ,
  • Sharof Khudayberdiev, Philipps-Universität Marburg
  • ,
  • Ayla Aksoy-Aksel, Philipps-Universität Marburg
  • ,
  • Silvia Bicker, Philipps-Universität Marburg
  • ,
  • Cristina Ruedell Reschke, Royal Coll Surgeons Ireland, Royal College of Surgeons - Ireland
  • ,
  • Rana Raoof, Royal Coll Surgeons Ireland, Royal College of Surgeons - Ireland
  • ,
  • Gary P. Brennan, Royal Coll Surgeons Ireland, Royal College of Surgeons - Ireland
  • ,
  • Norman Delanty, Beaumont Hospital
  • ,
  • Michael A. Farrell, Beaumont Hospital
  • ,
  • Donncha F. O'Brien, Beaumont Hospital
  • ,
  • Sebastian Bauer, Goethe-Universität Frankfurt, Philipps-Universität Marburg
  • ,
  • Braxton Norwood, Philipps-Universität Marburg, Goethe-Universität Frankfurt
  • ,
  • Morten T. Veno
  • ,
  • Marcus Krueger, University of Cologne
  • ,
  • Thomas Braun, Max Planck Institute
  • ,
  • Jorgen Kjems
  • Felix Rosenow, Philipps-Universität Marburg, Goethe-Universität Frankfurt
  • ,
  • David C. Henshall, Royal Coll Surgeons Ireland, Royal College of Surgeons - Ireland
  • ,
  • Christoph Dieterich, HIT, University Hospital, Heidelberg
  • ,
  • Gerhard Schratt, Philipps-Universität Marburg

Synaptic downscaling is a homeostatic mechanism that allows neurons to reduce firing rates during chronically elevated network activity. Although synaptic downscaling is important in neural circuit development and epilepsy, the underlying mechanisms are poorly described. We performed small RNA profiling in picrotoxin (PTX)-treated hippocampal neurons, a model of synaptic downscaling. Thereby, we identified eight microRNAs (miRNAs) that were increased in response to PTX, including miR-129-5p, whose inhibition blocked synaptic downscaling in vitro and reduced epileptic seizure severity in vivo. Using transcriptome, proteome, and bioinformatic analysis, we identified the calcium pump Atp2b4 and doublecortin (Dcx) as miR-129-5p targets. Restoring Atp2b4 and Dcx expression was sufficient to prevent synaptic downscaling in PTX-treated neurons. Furthermore, we characterized a functional crosstalk between miR-129-5p and the RNA-binding protein (RBP) Rbfox1. In the absence of PTX, Rbfox1 promoted the expression of Atp2b4 and Dcx. Upon PTX treatment, Rbfox1 expression was downregulated by miR-129-5p, thereby allowing the repression of Atp2b4 and Dcx. We therefore identified a novel activitydependent miRNA/RBP crosstalk during synaptic scaling, with potential implications for neural network homeostasis and epileptogenesis.

Original languageEnglish
JournalE M B O Journal
Volume36
Issue12
Pages (from-to)1770-1787
Number of pages18
ISSN0261-4189
DOIs
Publication statusPublished - 14 Jun 2017

    Research areas

  • epilepsy, homeostatic plasticity, microRNA, RNA-binding protein, synaptic scaling, DENDRITIC SPINE DEVELOPMENT, MESSENGER-RNA LOCALIZATION, SYNAPTIC PLASTICITY, STATUS EPILEPTICUS, MAMMALIAN BRAIN, AMPA RECEPTORS, VISUAL-CORTEX, EXPRESSION, MICRORNAS, PROTEINS

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