A Melt-electrowritten Filter for Capture and Culture of Circulating Colon Cancer Cells

Mathias Lindh Jørgensen; Christoph Alexander Müller; Mille Sikkersoq Kimer Nøddesbo; Marcin Nadzieja; Zhongyang Zhang; Yingchun Su; Jesper Just; Karen-Lise Garm Spindler; Menglin Chen

doi:10.1016/j.mtbio.2020.100052

A Melt-electrowritten Filter for Capture and Culture of Circulating Colon Cancer Cells

Mathias Lindh Jørgensen, Christoph Alexander Müller, Mille Sikkersoq Kimer Nøddesbo, Marcin Nadzieja, Zhongyang Zhang, Yingchun Su, Jesper Just, Karen-Lise Garm Spindler, Menglin Chen

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

16 Citations (Scopus)

Abstract

Metastasis is the major cause of death in cancer patients accounting for about 90% of the mortality. The detection and analysis of the hallmark of metastasis, circulating tumor cells (CTCs), have significant impact in cancer biology and clinical practice. However, the scarcity of CTCs in blood, particularly in that of colorectal cancer patients, is a serious bottleneck in the development of CTC-based precision medicine. Herein, the melt electrowriting (MEW) technology was used for reproductive fabrication of a biocompatible antibody-presenting polycaprolactone filter with tailored porous structure. It is demonstrated, for the first time, that such filter can be used not only to catch cancer cells spiked in whole blood but also to culture the cancer cells directly on site. Specifically, HT29 colon cancer cells can be captured with an efficiency of 85%, and when spiked into 4 mL of whole blood, 47% were captured on one Ø12mm filter. Furthermore, repeated capture and culture experiments have shown that as few as 20 HT29 colon cancer cells spiked into 4 mL of whole blood can be captured on the filter and within 2 weeks be expanded on site to become tumor bodies that are visible to the untrained eye. This filter allows for downstream analysis, such as flow cytometry, immunocytochemistry, Western blotting, and rt-qPCR. This technology represents a simple and cost-effective platform that potentially enables fast and efficient culture of rare CTCs from patients’ blood. This provides non-invasive alternatives for solid biopsy tumor materials for treatment screening, with great potential to realize precision medicine for cancer treatment.

Original language	English
Article number	100052
Journal	Materials Today Bio
Volume	6
Number of pages	10
DOIs	https://doi.org/10.1016/j.mtbio.2020.100052
Publication status	Published - Mar 2020

Keywords

Bioconjugation
Circulating tumor cells
Colorectal cancer
Melt electrospinning writing
On-site culture
Polycaprolactone
EX-VIVO CULTURE
COLORECTAL-CANCER
EXPANSION
TUMOR-CELLS
FIBROCYTES
CTCS

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10.1016/j.mtbio.2020.100052

Cite this

@article{1059e86172044056aff21f8888e201bc,

title = "A Melt-electrowritten Filter for Capture and Culture of Circulating Colon Cancer Cells",

abstract = "Metastasis is the major cause of death in cancer patients accounting for about 90% of the mortality. The detection and analysis of the hallmark of metastasis, circulating tumor cells (CTCs), have significant impact in cancer biology and clinical practice. However, the scarcity of CTCs in blood, particularly in that of colorectal cancer patients, is a serious bottleneck in the development of CTC-based precision medicine. Herein, the melt electrowriting (MEW) technology was used for reproductive fabrication of a biocompatible antibody-presenting polycaprolactone filter with tailored porous structure. It is demonstrated, for the first time, that such filter can be used not only to catch cancer cells spiked in whole blood but also to culture the cancer cells directly on site. Specifically, HT29 colon cancer cells can be captured with an efficiency of 85%, and when spiked into 4 mL of whole blood, 47% were captured on one {\O}12mm filter. Furthermore, repeated capture and culture experiments have shown that as few as 20 HT29 colon cancer cells spiked into 4 mL of whole blood can be captured on the filter and within 2 weeks be expanded on site to become tumor bodies that are visible to the untrained eye. This filter allows for downstream analysis, such as flow cytometry, immunocytochemistry, Western blotting, and rt-qPCR. This technology represents a simple and cost-effective platform that potentially enables fast and efficient culture of rare CTCs from patients{\textquoteright} blood. This provides non-invasive alternatives for solid biopsy tumor materials for treatment screening, with great potential to realize precision medicine for cancer treatment.",

keywords = "Bioconjugation, Circulating tumor cells, Colorectal cancer, Melt electrospinning writing, On-site culture, Polycaprolactone, EX-VIVO CULTURE, COLORECTAL-CANCER, EXPANSION, TUMOR-CELLS, FIBROCYTES, CTCS",

author = "J{\o}rgensen, {Mathias Lindh} and M{\"u}ller, {Christoph Alexander} and N{\o}ddesbo, {Mille Sikkersoq Kimer} and Marcin Nadzieja and Zhongyang Zhang and Yingchun Su and Jesper Just and Spindler, {Karen-Lise Garm} and Menglin Chen",

note = "{\textcopyright} 2020 The Author(s).",

year = "2020",

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doi = "10.1016/j.mtbio.2020.100052",

language = "English",

volume = "6",

journal = "Materials Today Bio",

issn = "2590-0064",

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A Melt-electrowritten Filter for Capture and Culture of Circulating Colon Cancer Cells. / Jørgensen, Mathias Lindh; Müller, Christoph Alexander; Nøddesbo, Mille Sikkersoq Kimer et al.
In: Materials Today Bio, Vol. 6, 100052, 03.2020.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - A Melt-electrowritten Filter for Capture and Culture of Circulating Colon Cancer Cells

AU - Jørgensen, Mathias Lindh

AU - Müller, Christoph Alexander

AU - Nøddesbo, Mille Sikkersoq Kimer

AU - Nadzieja, Marcin

AU - Zhang, Zhongyang

AU - Su, Yingchun

AU - Just, Jesper

AU - Spindler, Karen-Lise Garm

AU - Chen, Menglin

PY - 2020/3

Y1 - 2020/3

N2 - Metastasis is the major cause of death in cancer patients accounting for about 90% of the mortality. The detection and analysis of the hallmark of metastasis, circulating tumor cells (CTCs), have significant impact in cancer biology and clinical practice. However, the scarcity of CTCs in blood, particularly in that of colorectal cancer patients, is a serious bottleneck in the development of CTC-based precision medicine. Herein, the melt electrowriting (MEW) technology was used for reproductive fabrication of a biocompatible antibody-presenting polycaprolactone filter with tailored porous structure. It is demonstrated, for the first time, that such filter can be used not only to catch cancer cells spiked in whole blood but also to culture the cancer cells directly on site. Specifically, HT29 colon cancer cells can be captured with an efficiency of 85%, and when spiked into 4 mL of whole blood, 47% were captured on one Ø12mm filter. Furthermore, repeated capture and culture experiments have shown that as few as 20 HT29 colon cancer cells spiked into 4 mL of whole blood can be captured on the filter and within 2 weeks be expanded on site to become tumor bodies that are visible to the untrained eye. This filter allows for downstream analysis, such as flow cytometry, immunocytochemistry, Western blotting, and rt-qPCR. This technology represents a simple and cost-effective platform that potentially enables fast and efficient culture of rare CTCs from patients’ blood. This provides non-invasive alternatives for solid biopsy tumor materials for treatment screening, with great potential to realize precision medicine for cancer treatment.

AB - Metastasis is the major cause of death in cancer patients accounting for about 90% of the mortality. The detection and analysis of the hallmark of metastasis, circulating tumor cells (CTCs), have significant impact in cancer biology and clinical practice. However, the scarcity of CTCs in blood, particularly in that of colorectal cancer patients, is a serious bottleneck in the development of CTC-based precision medicine. Herein, the melt electrowriting (MEW) technology was used for reproductive fabrication of a biocompatible antibody-presenting polycaprolactone filter with tailored porous structure. It is demonstrated, for the first time, that such filter can be used not only to catch cancer cells spiked in whole blood but also to culture the cancer cells directly on site. Specifically, HT29 colon cancer cells can be captured with an efficiency of 85%, and when spiked into 4 mL of whole blood, 47% were captured on one Ø12mm filter. Furthermore, repeated capture and culture experiments have shown that as few as 20 HT29 colon cancer cells spiked into 4 mL of whole blood can be captured on the filter and within 2 weeks be expanded on site to become tumor bodies that are visible to the untrained eye. This filter allows for downstream analysis, such as flow cytometry, immunocytochemistry, Western blotting, and rt-qPCR. This technology represents a simple and cost-effective platform that potentially enables fast and efficient culture of rare CTCs from patients’ blood. This provides non-invasive alternatives for solid biopsy tumor materials for treatment screening, with great potential to realize precision medicine for cancer treatment.

KW - Bioconjugation

KW - Circulating tumor cells

KW - Colorectal cancer

KW - Melt electrospinning writing

KW - On-site culture

KW - Polycaprolactone

KW - EX-VIVO CULTURE

KW - COLORECTAL-CANCER

KW - EXPANSION

KW - TUMOR-CELLS

KW - FIBROCYTES

KW - CTCS

U2 - 10.1016/j.mtbio.2020.100052

DO - 10.1016/j.mtbio.2020.100052

M3 - Journal article

C2 - 32490373

SN - 2590-0064

VL - 6

JO - Materials Today Bio

JF - Materials Today Bio

M1 - 100052

ER -

A Melt-electrowritten Filter for Capture and Culture of Circulating Colon Cancer Cells

Abstract

Keywords

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