A major lineage of enteroendocrine cells coexpress CCK, secretin, GIP, GLP-1, PYY, and neurotensin but not somatostatin

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  • Kristoffer Lihme Egerod, Pharmacology, Denmark
  • Maja Storm Engelstoft, Pharmacology, Denmark
  • Kaare Villum Grunddal, Metabolic Receptology, Denmark
  • Mark Klitgaard Nøhr, Metabolic Receptology, Denmark
  • Anna Elisabet Lilja Secher, 2012 Institut for Farmakologi og Farmakoterapi, Denmark
  • Ichiro Sakata, Department of Internal Medicine, University of Texas Southwestern Medical Center, United States
  • Jens Pedersen, Faculty of Health Sciences, University of Copenhagen, Denmark
  • Johanne A Windeløv
  • ,
  • Ernst-Martin Füchtbauer
  • Jørgen Olsen, Denmark
  • Frank Sundler, Lund University Diabetes Center, Sweden
  • Jan P Christensen, Denmark
  • Nils Wierup, Lund University Diabetes Center, Sweden
  • Jesper V Olsen, Denmark
  • Jens Juul Holst, Afd. for Endokrinologisk Forskning, Denmark
  • Jeffrey M Zigman, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, United States
  • Steen S Poulsen, Denmark
  • Thue W. Schwartz, Institut for Neurovidenskab og Farmakologi, Denmark
Enteroendocrine cells such as duodenal cholecystokinin (CCK cells) are generally thought to be confined to certain segments of the gastrointestinal (GI) tract and to store and release peptides derived from only a single peptide precursor. In the current study, however, transgenic mice expressing enhanced green fluorescent protein (eGFP) under the control of the CCK promoter demonstrated a distribution pattern of CCK-eGFP positive cells that extended throughout the intestine. Quantitative PCR and liquid chromatography-mass spectrometry proteomic analyses of isolated, FACS-purified CCK-eGFP-positive cells demonstrated expression of not only CCK but also glucagon-like peptide 1 (GLP-1), gastric inhibitory peptide (GIP), peptide YY (PYY), neurotensin, and secretin, but not somatostatin. Immunohistochemistry confirmed this expression pattern. The broad coexpression phenomenon was observed both in crypts and villi as demonstrated by immunohistochemistry and FACS analysis of separated cell populations. Single-cell quantitative PCR indicated that approximately half of the duodenal CCK-eGFP cells express one peptide precursor in addition to CCK, whereas an additional smaller fraction expresses two peptide precursors in addition to CCK. The coexpression pattern was further confirmed through a cell ablation study based on expression of the human diphtheria toxin receptor under the control of the proglucagon promoter, in which activation of the receptor resulted in a marked reduction not only in GLP-1 cells, but also PYY, neurotensin, GIP, CCK, and secretin cells, whereas somatostatin cells were spared. Key elements of the coexpression pattern were confirmed by immunohistochemical double staining in human small intestine. It is concluded that a lineage of mature enteroendocrine cells have the ability to coexpress members of a group of functionally related peptides: CCK, secretin, GIP, GLP-1, PYY, and neurotensin, suggesting a potential therapeutic target for the treatment and prevention of diabetes and obesity.
Original languageEnglish
Book seriesContemporary Endocrinology
Volume153
Issue12
Pages (from-to)5782-5795
Number of pages14
ISSN0196-8653
DOIs
Publication statusPublished - 2012

    Research areas

  • Animals, Cell Lineage, Cell Separation, Cholecystokinin, Diabetes Mellitus, Enteroendocrine Cells, Flow Cytometry, Gastric Inhibitory Polypeptide, Gene Expression Regulation, Ghrelin, Glucagon-Like Peptide 1, Green Fluorescent Proteins, Humans, Immunohistochemistry, Intestines, Mice, Mice, Transgenic, Neurotensin, Obesity, Peptide YY, Promoter Regions, Genetic

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