A low-cost open-source 3D-printed mouse cradle suspension system for awake or anaesthetised 1H/31P magnetic resonance  spectroscopy.

Saba Molhemi; Leif Østergaard; Brian Hansen

doi:10.1016/j.ohx.2024.e00616

A low-cost open-source 3D-printed mouse cradle suspension system for awake or anaesthetised 1H/31P magnetic resonance spectroscopy.

Saba Molhemi^*, Leif Østergaard, Brian Hansen

^*Corresponding author for this work

Department of Clinical Medicine - Center of Functionally Integrative Neuroscience

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Abstract

Awake mouse MRI and spectroscopy (MRS) are valuable techniques for studying biological questions without the confounding effects of anaesthesia. Currently, no off-the-shelf solution exists for awake mouse MRI/S. To address this, we present a Mouse Cradle Suspension System (MCSS) for awake mouse MRI/S. Our design is freely available and offers a low-cost 3D-printed setup compatible with a Bruker Biospec 94/20 scanner and commercially available ¹H/³¹P surface- and volume-coils, such as coils from Bruker Biospin (T20025V3) and Rapid (O-XL-HL-094). While the focus here is measurements in awake mouse brain, the coils and the presented setup is suitable for both mouse and rat brain, and studies of mouse body organs. Moreover, the design is easily modifiable to suit other applications and hardware configurations. The MCSS reduces gradient-induced coil vibrations and supports cross-coil setups. It features an inner and outer rail system for easy insertion of the coil and customized mouse cradle into the scanner. The cradle is suitable for both anaesthetized and awake mouse scans and existing habituation protocols for awake mouse MRI/S. This MCSS design ensures a smooth workflow for awake mouse MRI/S. The cost is approximately 200€, achieved using 3D-printed and off-the-shelf components.

Original language	English
Article number	e00616
Journal	HardwareX
Volume	21
ISSN	2468-0672
DOIs	https://doi.org/10.1016/j.ohx.2024.e00616
Publication status	Published - Mar 2025

Keywords

3D printing
Awake mouse
Computer-aided design
Phosphorus magnetic resonance spectroscopy
Preclinical
Proton magnetic resonance spectroscopy

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title = "A low-cost open-source 3D-printed mouse cradle suspension system for awake or anaesthetised 1H/31P magnetic resonance spectroscopy.",

abstract = "Awake mouse MRI and spectroscopy (MRS) are valuable techniques for studying biological questions without the confounding effects of anaesthesia. Currently, no off-the-shelf solution exists for awake mouse MRI/S. To address this, we present a Mouse Cradle Suspension System (MCSS) for awake mouse MRI/S. Our design is freely available and offers a low-cost 3D-printed setup compatible with a Bruker Biospec 94/20 scanner and commercially available 1H/31P surface- and volume-coils, such as coils from Bruker Biospin (T20025V3) and Rapid (O-XL-HL-094). While the focus here is measurements in awake mouse brain, the coils and the presented setup is suitable for both mouse and rat brain, and studies of mouse body organs. Moreover, the design is easily modifiable to suit other applications and hardware configurations. The MCSS reduces gradient-induced coil vibrations and supports cross-coil setups. It features an inner and outer rail system for easy insertion of the coil and customized mouse cradle into the scanner. The cradle is suitable for both anaesthetized and awake mouse scans and existing habituation protocols for awake mouse MRI/S. This MCSS design ensures a smooth workflow for awake mouse MRI/S. The cost is approximately 200€, achieved using 3D-printed and off-the-shelf components.",

keywords = "3D printing, Awake mouse, Computer-aided design, Phosphorus magnetic resonance spectroscopy, Preclinical, Proton magnetic resonance spectroscopy",

author = "Saba Molhemi and Leif {\O}stergaard and Brian Hansen",

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doi = "10.1016/j.ohx.2024.e00616",

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A low-cost open-source 3D-printed mouse cradle suspension system for awake or anaesthetised 1H/31P magnetic resonance spectroscopy. / Molhemi, Saba ; Østergaard, Leif ; Hansen, Brian.
In: HardwareX, Vol. 21, e00616, 03.2025.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - A low-cost open-source 3D-printed mouse cradle suspension system for awake or anaesthetised 1H/31P magnetic resonance spectroscopy.

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AU - Østergaard, Leif

AU - Hansen, Brian

PY - 2025/3

Y1 - 2025/3

N2 - Awake mouse MRI and spectroscopy (MRS) are valuable techniques for studying biological questions without the confounding effects of anaesthesia. Currently, no off-the-shelf solution exists for awake mouse MRI/S. To address this, we present a Mouse Cradle Suspension System (MCSS) for awake mouse MRI/S. Our design is freely available and offers a low-cost 3D-printed setup compatible with a Bruker Biospec 94/20 scanner and commercially available 1H/31P surface- and volume-coils, such as coils from Bruker Biospin (T20025V3) and Rapid (O-XL-HL-094). While the focus here is measurements in awake mouse brain, the coils and the presented setup is suitable for both mouse and rat brain, and studies of mouse body organs. Moreover, the design is easily modifiable to suit other applications and hardware configurations. The MCSS reduces gradient-induced coil vibrations and supports cross-coil setups. It features an inner and outer rail system for easy insertion of the coil and customized mouse cradle into the scanner. The cradle is suitable for both anaesthetized and awake mouse scans and existing habituation protocols for awake mouse MRI/S. This MCSS design ensures a smooth workflow for awake mouse MRI/S. The cost is approximately 200€, achieved using 3D-printed and off-the-shelf components.

AB - Awake mouse MRI and spectroscopy (MRS) are valuable techniques for studying biological questions without the confounding effects of anaesthesia. Currently, no off-the-shelf solution exists for awake mouse MRI/S. To address this, we present a Mouse Cradle Suspension System (MCSS) for awake mouse MRI/S. Our design is freely available and offers a low-cost 3D-printed setup compatible with a Bruker Biospec 94/20 scanner and commercially available 1H/31P surface- and volume-coils, such as coils from Bruker Biospin (T20025V3) and Rapid (O-XL-HL-094). While the focus here is measurements in awake mouse brain, the coils and the presented setup is suitable for both mouse and rat brain, and studies of mouse body organs. Moreover, the design is easily modifiable to suit other applications and hardware configurations. The MCSS reduces gradient-induced coil vibrations and supports cross-coil setups. It features an inner and outer rail system for easy insertion of the coil and customized mouse cradle into the scanner. The cradle is suitable for both anaesthetized and awake mouse scans and existing habituation protocols for awake mouse MRI/S. This MCSS design ensures a smooth workflow for awake mouse MRI/S. The cost is approximately 200€, achieved using 3D-printed and off-the-shelf components.

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KW - Awake mouse

KW - Computer-aided design

KW - Phosphorus magnetic resonance spectroscopy

KW - Preclinical

KW - Proton magnetic resonance spectroscopy

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JF - HardwareX

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A low-cost open-source 3D-printed mouse cradle suspension system for awake or anaesthetised 1H/31P magnetic resonance spectroscopy.

Abstract

Keywords

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