A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells

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Standard

A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells. / Hansson, Mattias; Olesen, Dorthe R; Peterslund, Janny M L; Engberg, Nina; Kahn, Morten Østergaard; Winzi, Maria Karin; Klein, Tino; Serup, Palle; Maddox-Hyttel, Poul.

In: Advances in Developmental Biology, Vol. 330, No. 2, 15.06.2009, p. 286-304.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Hansson, M, Olesen, DR, Peterslund, JML, Engberg, N, Kahn, MØ, Winzi, MK, Klein, T, Serup, P & Maddox-Hyttel, P 2009, 'A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells', Advances in Developmental Biology, vol. 330, no. 2, pp. 286-304. https://doi.org/10.1016/j.ydbio.2009.03.026

APA

Hansson, M., Olesen, D. R., Peterslund, J. M. L., Engberg, N., Kahn, M. Ø., Winzi, M. K., ... Maddox-Hyttel, P. (2009). A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells. Advances in Developmental Biology, 330(2), 286-304. https://doi.org/10.1016/j.ydbio.2009.03.026

CBE

Hansson M, Olesen DR, Peterslund JML, Engberg N, Kahn MØ, Winzi MK, Klein T, Serup P, Maddox-Hyttel P. 2009. A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells. Advances in Developmental Biology. 330(2):286-304. https://doi.org/10.1016/j.ydbio.2009.03.026

MLA

Vancouver

Hansson M, Olesen DR, Peterslund JML, Engberg N, Kahn MØ, Winzi MK et al. A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells. Advances in Developmental Biology. 2009 Jun 15;330(2):286-304. https://doi.org/10.1016/j.ydbio.2009.03.026

Author

Hansson, Mattias ; Olesen, Dorthe R ; Peterslund, Janny M L ; Engberg, Nina ; Kahn, Morten Østergaard ; Winzi, Maria Karin ; Klein, Tino ; Serup, Palle ; Maddox-Hyttel, Poul. / A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells. In: Advances in Developmental Biology. 2009 ; Vol. 330, No. 2. pp. 286-304.

Bibtex

@article{6ef62be286f34973a8fc3525f5fe9d60,
title = "A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells",
abstract = "Here we examine how BMP, Wnt, and FGF signaling modulate activin-induced mesendodermal differentiation of mouse ES cells grown under defined conditions in adherent monoculture. We monitor ES cells containing reporter genes for markers of primitive streak (PS) and its progeny and extend previous findings on the ability of increasing concentrations of activin to progressively induce more ES cell progeny to anterior PS and endodermal fates. We find that the number of Sox17- and Gsc-expressing cells increases with increasing activin concentration while the highest number of T-expressing cells is found at the lowest activin concentration. The expression of Gsc and other anterior markers induced by activin is prevented by treatment with BMP4, which induces T expression and subsequent mesodermal development. We show that canonical Wnt signaling is required only during late stages of activin-induced development of Sox17-expressing endodermal cells. Furthermore, Dkk1 treatment is less effective in reducing development of Sox17(+) endodermal cells in adherent culture than in aggregate culture and appears to inhibit nodal-mediated induction of Sox17(+) cells more effectively than activin-mediated induction. Notably, activin induction of Gsc-GFP(+) cells appears refractory to inhibition of canonical Wnt signaling but shows a dependence on early as well as late FGF signaling. Additionally, we find a late dependence on FGF signaling during induction of Sox17(+) cells by activin while BMP4-induced T expression requires FGF signaling in adherent but not aggregate culture. Lastly, we demonstrate that activin-induced definitive endoderm derived from mouse ES cells can incorporate into the developing foregut endoderm in vivo and adopt a mostly anterior foregut character after further culture in vitro.",
keywords = "Activins, Animals, Base Sequence, Cell Differentiation, Cells, Cultured, Chick Embryo, Embryonic Stem Cells, Endoderm, Fibroblast Growth Factors, Flow Cytometry, Fluorescent Antibody Technique, Mice, RNA, Small Interfering, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction, Wnt Proteins",
author = "Mattias Hansson and Olesen, {Dorthe R} and Peterslund, {Janny M L} and Nina Engberg and Kahn, {Morten {\O}stergaard} and Winzi, {Maria Karin} and Tino Klein and Palle Serup and Poul Maddox-Hyttel",
year = "2009",
month = "6",
day = "15",
doi = "10.1016/j.ydbio.2009.03.026",
language = "English",
volume = "330",
pages = "286--304",
journal = "Advances in Developmental Biology",
issn = "1574-3349",
publisher = "Elsevier BV",
number = "2",

}

RIS

TY - JOUR

T1 - A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells

AU - Hansson, Mattias

AU - Olesen, Dorthe R

AU - Peterslund, Janny M L

AU - Engberg, Nina

AU - Kahn, Morten Østergaard

AU - Winzi, Maria Karin

AU - Klein, Tino

AU - Serup, Palle

AU - Maddox-Hyttel, Poul

PY - 2009/6/15

Y1 - 2009/6/15

N2 - Here we examine how BMP, Wnt, and FGF signaling modulate activin-induced mesendodermal differentiation of mouse ES cells grown under defined conditions in adherent monoculture. We monitor ES cells containing reporter genes for markers of primitive streak (PS) and its progeny and extend previous findings on the ability of increasing concentrations of activin to progressively induce more ES cell progeny to anterior PS and endodermal fates. We find that the number of Sox17- and Gsc-expressing cells increases with increasing activin concentration while the highest number of T-expressing cells is found at the lowest activin concentration. The expression of Gsc and other anterior markers induced by activin is prevented by treatment with BMP4, which induces T expression and subsequent mesodermal development. We show that canonical Wnt signaling is required only during late stages of activin-induced development of Sox17-expressing endodermal cells. Furthermore, Dkk1 treatment is less effective in reducing development of Sox17(+) endodermal cells in adherent culture than in aggregate culture and appears to inhibit nodal-mediated induction of Sox17(+) cells more effectively than activin-mediated induction. Notably, activin induction of Gsc-GFP(+) cells appears refractory to inhibition of canonical Wnt signaling but shows a dependence on early as well as late FGF signaling. Additionally, we find a late dependence on FGF signaling during induction of Sox17(+) cells by activin while BMP4-induced T expression requires FGF signaling in adherent but not aggregate culture. Lastly, we demonstrate that activin-induced definitive endoderm derived from mouse ES cells can incorporate into the developing foregut endoderm in vivo and adopt a mostly anterior foregut character after further culture in vitro.

AB - Here we examine how BMP, Wnt, and FGF signaling modulate activin-induced mesendodermal differentiation of mouse ES cells grown under defined conditions in adherent monoculture. We monitor ES cells containing reporter genes for markers of primitive streak (PS) and its progeny and extend previous findings on the ability of increasing concentrations of activin to progressively induce more ES cell progeny to anterior PS and endodermal fates. We find that the number of Sox17- and Gsc-expressing cells increases with increasing activin concentration while the highest number of T-expressing cells is found at the lowest activin concentration. The expression of Gsc and other anterior markers induced by activin is prevented by treatment with BMP4, which induces T expression and subsequent mesodermal development. We show that canonical Wnt signaling is required only during late stages of activin-induced development of Sox17-expressing endodermal cells. Furthermore, Dkk1 treatment is less effective in reducing development of Sox17(+) endodermal cells in adherent culture than in aggregate culture and appears to inhibit nodal-mediated induction of Sox17(+) cells more effectively than activin-mediated induction. Notably, activin induction of Gsc-GFP(+) cells appears refractory to inhibition of canonical Wnt signaling but shows a dependence on early as well as late FGF signaling. Additionally, we find a late dependence on FGF signaling during induction of Sox17(+) cells by activin while BMP4-induced T expression requires FGF signaling in adherent but not aggregate culture. Lastly, we demonstrate that activin-induced definitive endoderm derived from mouse ES cells can incorporate into the developing foregut endoderm in vivo and adopt a mostly anterior foregut character after further culture in vitro.

KW - Activins

KW - Animals

KW - Base Sequence

KW - Cell Differentiation

KW - Cells, Cultured

KW - Chick Embryo

KW - Embryonic Stem Cells

KW - Endoderm

KW - Fibroblast Growth Factors

KW - Flow Cytometry

KW - Fluorescent Antibody Technique

KW - Mice

KW - RNA, Small Interfering

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Signal Transduction

KW - Wnt Proteins

U2 - 10.1016/j.ydbio.2009.03.026

DO - 10.1016/j.ydbio.2009.03.026

M3 - Journal article

VL - 330

SP - 286

EP - 304

JO - Advances in Developmental Biology

JF - Advances in Developmental Biology

SN - 1574-3349

IS - 2

ER -