A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2

William Garland; Itys Comet; Mengjun Wu; Aliaksandra Radzisheuskaya; Leonor Rib; Kristoffer Vitting-Seerup; Marta Lloret-Llinares; Albin Sandelin; Kristian Helin; Torben Heick Jensen

doi:10.1016/j.celrep.2019.10.011

A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2

William Garland, Itys Comet, Mengjun Wu, Aliaksandra Radzisheuskaya, Leonor Rib, Kristoffer Vitting-Seerup, Marta Lloret-Llinares, Albin Sandelin, Kristian Helin^*, Torben Heick Jensen

^*Corresponding author for this work

Department of Molecular Biology and Genetics - RNA Biology and Innovation

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Abstract

ZFC3H1 targets pA⁺ RNA for decay by the nuclear RNA exosome. Garland et al. report a disruptive relationship between excess RNA and PRC2 upon depletion of ZFC3H1 in mouse ESCs. In such conditions, RNA is bound by PRC2 components, which show reduced binding to chromatin and fellow PRC2 proteins.

Original language	English
Journal	Cell Reports
Volume	29
Issue	7
Pages (from-to)	1800-1811.e6
Number of pages	18
ISSN	2211-1247
DOIs	https://doi.org/10.1016/j.celrep.2019.10.011
Publication status	Published - Nov 2019

Keywords

chromatin
H3K27 methylation
nuclear RNA decay
PAXT
PRC2
RNA exosome
stem cell regulation
READ ALIGNMENT
DEVELOPMENTAL REGULATORS
CHROMATIN
DNA METHYLATION
EXOSOME
PLURIPOTENCY
GENE-EXPRESSION
DIFFERENTIAL EXPRESSION ANALYSIS
PROTEIN EED
RNA, Nuclear/genetics
Polycomb Repressive Complex 2/genetics
Transcription, Genetic
Transcription Factors/genetics
RNA Stability
Mouse Embryonic Stem Cells/cytology
Mice, Knockout
Animals
Chromatin/genetics
Mice

Access to Document

10.1016/j.celrep.2019.10.011

Cite this

@article{01bca3b2bb6f40fe86ad057ae6e37922,

title = "A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2",

abstract = "ZFC3H1 targets pA+ RNA for decay by the nuclear RNA exosome. Garland et al. report a disruptive relationship between excess RNA and PRC2 upon depletion of ZFC3H1 in mouse ESCs. In such conditions, RNA is bound by PRC2 components, which show reduced binding to chromatin and fellow PRC2 proteins.",

keywords = "chromatin, H3K27 methylation, nuclear RNA decay, PAXT, PRC2, RNA exosome, stem cell regulation, READ ALIGNMENT, DEVELOPMENTAL REGULATORS, CHROMATIN, DNA METHYLATION, EXOSOME, PLURIPOTENCY, GENE-EXPRESSION, DIFFERENTIAL EXPRESSION ANALYSIS, PROTEIN EED, RNA, Nuclear/genetics, Polycomb Repressive Complex 2/genetics, Transcription, Genetic, Transcription Factors/genetics, RNA Stability, Mouse Embryonic Stem Cells/cytology, Mice, Knockout, Animals, Chromatin/genetics, Mice",

author = "William Garland and Itys Comet and Mengjun Wu and Aliaksandra Radzisheuskaya and Leonor Rib and Kristoffer Vitting-Seerup and Marta Lloret-Llinares and Albin Sandelin and Kristian Helin and Jensen, {Torben Heick}",

year = "2019",

month = nov,

doi = "10.1016/j.celrep.2019.10.011",

language = "English",

volume = "29",

pages = "1800--1811.e6",

journal = "Cell Reports",

issn = "2211-1247",

publisher = "Cell Press",

number = "7",

}

A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2. / Garland, William; Comet, Itys; Wu, Mengjun et al.
In: Cell Reports, Vol. 29, No. 7, 11.2019, p. 1800-1811.e6.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2

AU - Garland, William

AU - Comet, Itys

AU - Wu, Mengjun

AU - Radzisheuskaya, Aliaksandra

AU - Rib, Leonor

AU - Vitting-Seerup, Kristoffer

AU - Lloret-Llinares, Marta

AU - Sandelin, Albin

AU - Helin, Kristian

AU - Jensen, Torben Heick

PY - 2019/11

Y1 - 2019/11

N2 - ZFC3H1 targets pA+ RNA for decay by the nuclear RNA exosome. Garland et al. report a disruptive relationship between excess RNA and PRC2 upon depletion of ZFC3H1 in mouse ESCs. In such conditions, RNA is bound by PRC2 components, which show reduced binding to chromatin and fellow PRC2 proteins.

AB - ZFC3H1 targets pA+ RNA for decay by the nuclear RNA exosome. Garland et al. report a disruptive relationship between excess RNA and PRC2 upon depletion of ZFC3H1 in mouse ESCs. In such conditions, RNA is bound by PRC2 components, which show reduced binding to chromatin and fellow PRC2 proteins.

KW - chromatin

KW - H3K27 methylation

KW - nuclear RNA decay

KW - PAXT

KW - PRC2

KW - RNA exosome

KW - stem cell regulation

KW - READ ALIGNMENT

KW - DEVELOPMENTAL REGULATORS

KW - CHROMATIN

KW - DNA METHYLATION

KW - EXOSOME

KW - PLURIPOTENCY

KW - GENE-EXPRESSION

KW - DIFFERENTIAL EXPRESSION ANALYSIS

KW - PROTEIN EED

KW - RNA, Nuclear/genetics

KW - Polycomb Repressive Complex 2/genetics

KW - Transcription, Genetic

KW - Transcription Factors/genetics

KW - RNA Stability

KW - Mouse Embryonic Stem Cells/cytology

KW - Mice, Knockout

KW - Animals

KW - Chromatin/genetics

KW - Mice

UR - http://www.scopus.com/inward/record.url?scp=85074652730&partnerID=8YFLogxK

U2 - 10.1016/j.celrep.2019.10.011

DO - 10.1016/j.celrep.2019.10.011

M3 - Journal article

C2 - 31722198

AN - SCOPUS:85074652730

SN - 2211-1247

VL - 29

SP - 1800-1811.e6

JO - Cell Reports

JF - Cell Reports

IS - 7

ER -

A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this