A DNA-based assay for digoxin detection

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The most common method for quantifying small-molecule drugs in blood samples is by liquid chromatography in combination with mass spectrometry. Few immuno-based assays are available for the detection of small-molecule drugs in blood. Here we report on a homogeneous assay that enables detection of the concentration of digoxin spiked into in a plasma sample. The assay is based on a shift in the equilibrium of a DNA strand displacement competition reaction, and can be performed in 30 min for concentrations above 10 nM. The equilibrium shift occurs upon binding of anti-digoxigenin antibody. As a model, the assay provides a potential alternative to current small-molecule detection methods used for therapeutic drug monitoring.

Original languageEnglish
Pages (from-to)19-30
Number of pages12
Publication statusPublished - 6 Mar 2018

    Research areas

  • Detection in plasma, Detection of small molecules, Digoxin detection, DNA nanotechnology, DNA strand displacement competition reaction, Homogeneous assay

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