A DNA tweezer-actuated enzyme nanoreactor

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A DNA tweezer-actuated enzyme nanoreactor. / Liu, Minghui; Fu, J.; Hejesen, Christian; Yang, Yuhe; Woodbury, Neal W.; Gothelf, K.; Liu, Yan; Yan, Hao.

In: Nature Communications, Vol. 4, 2127, 03.07.2013.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Liu, M, Fu, J, Hejesen, C, Yang, Y, Woodbury, NW, Gothelf, K, Liu, Y & Yan, H 2013, 'A DNA tweezer-actuated enzyme nanoreactor', Nature Communications, vol. 4, 2127. https://doi.org/10.1038/ncomms3127

APA

Liu, M., Fu, J., Hejesen, C., Yang, Y., Woodbury, N. W., Gothelf, K., ... Yan, H. (2013). A DNA tweezer-actuated enzyme nanoreactor. Nature Communications, 4, [2127]. https://doi.org/10.1038/ncomms3127

CBE

Liu M, Fu J, Hejesen C, Yang Y, Woodbury NW, Gothelf K, Liu Y, Yan H. 2013. A DNA tweezer-actuated enzyme nanoreactor. Nature Communications. 4. https://doi.org/10.1038/ncomms3127

MLA

Liu, Minghui et al. "A DNA tweezer-actuated enzyme nanoreactor". Nature Communications. 2013. 4. https://doi.org/10.1038/ncomms3127

Vancouver

Liu M, Fu J, Hejesen C, Yang Y, Woodbury NW, Gothelf K et al. A DNA tweezer-actuated enzyme nanoreactor. Nature Communications. 2013 Jul 3;4. 2127. https://doi.org/10.1038/ncomms3127

Author

Liu, Minghui ; Fu, J. ; Hejesen, Christian ; Yang, Yuhe ; Woodbury, Neal W. ; Gothelf, K. ; Liu, Yan ; Yan, Hao. / A DNA tweezer-actuated enzyme nanoreactor. In: Nature Communications. 2013 ; Vol. 4.

Bibtex

@article{e868e8ce5abc43b6a849401912a632ca,
title = "A DNA tweezer-actuated enzyme nanoreactor",
abstract = "The functions of regulatory enzymes are essential to modulating cellular pathways. Here we report a tweezer-like DNA nanodevice to actuate the activity of an enzyme/cofactor pair. A dehydrogenase and NAD + cofactor are attached to different arms of the DNA tweezer structure and actuation of enzymatic function is achieved by switching the tweezers between open and closed states. The enzyme/cofactor pair is spatially separated in the open state with inhibited enzyme function, whereas in the closed state, enzyme is activated by the close proximity of the two molecules. The conformational state of the DNA tweezer is controlled by the addition of specific oligonucleotides that serve as the thermodynamic driver (fuel) to trigger the change. Using this approach, several cycles of externally controlled enzyme inhibition and activation are successfully demonstrated. This principle of responsive enzyme nanodevices may be used to regulate other types of enzymes and to introduce feedback or feed-forward control loops.",
author = "Minghui Liu and J. Fu and Christian Hejesen and Yuhe Yang and Woodbury, {Neal W.} and K. Gothelf and Yan Liu and Hao Yan",
year = "2013",
month = "7",
day = "3",
doi = "10.1038/ncomms3127",
language = "English",
volume = "4",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",

}

RIS

TY - JOUR

T1 - A DNA tweezer-actuated enzyme nanoreactor

AU - Liu, Minghui

AU - Fu, J.

AU - Hejesen, Christian

AU - Yang, Yuhe

AU - Woodbury, Neal W.

AU - Gothelf, K.

AU - Liu, Yan

AU - Yan, Hao

PY - 2013/7/3

Y1 - 2013/7/3

N2 - The functions of regulatory enzymes are essential to modulating cellular pathways. Here we report a tweezer-like DNA nanodevice to actuate the activity of an enzyme/cofactor pair. A dehydrogenase and NAD + cofactor are attached to different arms of the DNA tweezer structure and actuation of enzymatic function is achieved by switching the tweezers between open and closed states. The enzyme/cofactor pair is spatially separated in the open state with inhibited enzyme function, whereas in the closed state, enzyme is activated by the close proximity of the two molecules. The conformational state of the DNA tweezer is controlled by the addition of specific oligonucleotides that serve as the thermodynamic driver (fuel) to trigger the change. Using this approach, several cycles of externally controlled enzyme inhibition and activation are successfully demonstrated. This principle of responsive enzyme nanodevices may be used to regulate other types of enzymes and to introduce feedback or feed-forward control loops.

AB - The functions of regulatory enzymes are essential to modulating cellular pathways. Here we report a tweezer-like DNA nanodevice to actuate the activity of an enzyme/cofactor pair. A dehydrogenase and NAD + cofactor are attached to different arms of the DNA tweezer structure and actuation of enzymatic function is achieved by switching the tweezers between open and closed states. The enzyme/cofactor pair is spatially separated in the open state with inhibited enzyme function, whereas in the closed state, enzyme is activated by the close proximity of the two molecules. The conformational state of the DNA tweezer is controlled by the addition of specific oligonucleotides that serve as the thermodynamic driver (fuel) to trigger the change. Using this approach, several cycles of externally controlled enzyme inhibition and activation are successfully demonstrated. This principle of responsive enzyme nanodevices may be used to regulate other types of enzymes and to introduce feedback or feed-forward control loops.

UR - http://www.scopus.com/inward/record.url?scp=84880100051&partnerID=8YFLogxK

U2 - 10.1038/ncomms3127

DO - 10.1038/ncomms3127

M3 - Journal article

VL - 4

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 2127

ER -