A DNA-programmed liposome fusion cascade

Philipp M. G. Löffler; Oliver Ries; Alexander Rabe; Anders Hauge Okholm; Rasmus Peter Thomsen; Jørgen Kjems; Stefan Vogel

doi:10.1002/anie.201703243

A DNA-programmed liposome fusion cascade

Philipp M. G. Löffler, Oliver Ries, Alexander Rabe, Anders Hauge Okholm, Rasmus Peter Thomsen, Jørgen Kjems, Stefan Vogel

Interdisciplinary Nanoscience Center - INANO-MBG, iNANO-huset

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Abstract

Chemically engineered and functionalized nanoscale compartments are used in bottom-up synthetic biology to construct compartmentalized chemical processes. Progressively more complex designs demand spatial and temporal control over entrapped species. Here, we address this demand with a DNA-encoded design for the successive fusion of multiple liposome populations. Three individual stages of fusion are induced by orthogonally hybridizing sets of membrane-anchored oligonucleotides. Each fusion event leads to efficient content mixing and transfer of the recognition unit for the subsequent stage. In contrast to fusion-protein-dependent eukaryotic vesicle processing, this artificial fusion cascade exploits the versatile encoding potential of DNA hybridization and is generally applicable to small and giant unilamellar vesicles. This platform could thus enable numerous applications in artificial cellular systems and liposome-based synthetic pathways.

Original language	English
Journal	Angewandte Chemie International Edition
Volume	56
Issue	43
Pages (from-to)	13228–13231
Number of pages	4
ISSN	1433-7851
DOIs	https://doi.org/10.1002/anie.201703243
Publication status	Published - 16 Oct 2017

Keywords

giant unilamellar vesicles
liposomes
membrane fusion
self-assembly
vesicles

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AU - Löffler, Philipp M. G.

AU - Ries, Oliver

AU - Rabe, Alexander

AU - Okholm, Anders Hauge

AU - Thomsen, Rasmus Peter

AU - Kjems, Jørgen

AU - Vogel, Stefan

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N2 - Chemically engineered and functionalized nanoscale compartments are used in bottom-up synthetic biology to construct compartmentalized chemical processes. Progressively more complex designs demand spatial and temporal control over entrapped species. Here, we address this demand with a DNA-encoded design for the successive fusion of multiple liposome populations. Three individual stages of fusion are induced by orthogonally hybridizing sets of membrane-anchored oligonucleotides. Each fusion event leads to efficient content mixing and transfer of the recognition unit for the subsequent stage. In contrast to fusion-protein-dependent eukaryotic vesicle processing, this artificial fusion cascade exploits the versatile encoding potential of DNA hybridization and is generally applicable to small and giant unilamellar vesicles. This platform could thus enable numerous applications in artificial cellular systems and liposome-based synthetic pathways.

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KW - giant unilamellar vesicles

KW - liposomes

KW - membrane fusion

KW - self-assembly

KW - vesicles

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DO - 10.1002/anie.201703243

M3 - Journal article

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JO - Angewandte Chemie International Edition

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A DNA-programmed liposome fusion cascade

Abstract

Keywords

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