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A comparison of conventional and computer-assisted semen analysis (CRISMAS software) using samples from 166 young Danish men

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A comparison of conventional and computer-assisted semen analysis (CRISMAS software) using samples from 166 young Danish men. / Vested, Anne; Ramlau-Hansen, Cecilia H; Bonde, Jens P; Thulstrup, Ane M; Kristensen, Susanne Lund; Toft, Gunnar.

In: Asian Journal of Andrology, Vol. 13, No. 3, 2011, p. 453-8.

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@article{3ef5cffdac844444bb4c3fc3664b9ea6,
title = "A comparison of conventional and computer-assisted semen analysis (CRISMAS software) using samples from 166 young Danish men",
abstract = "The aim of the present study was to compare assessments of sperm concentration and sperm motility analysed by conventional semen analysis with those obtained by computer-assisted semen analysis (CASA) (Copenhagen Rigshospitalet Image House Sperm Motility Analysis System (CRISMAS) 4.6 software) using semen samples from 166 young Danish men. The CRISMAS software identifies sperm concentration and classifies spermatozoa into three motility categories. To enable comparison of the two methods, the four motility stages obtained by conventional semen analysis were, based on their velocity classifications, divided into three stages, comparable to the three CRISMAS motility categories: rapidly progressive (A), slowly progressive (B) and non-progressive (C+D). Differences between the two methods were large for all investigated parameters (P <0.001). CRISMAS overestimated sperm concentration and the proportion of rapidly progressive spermatozoa and, consequently, underestimated the percentages of slowly progressive and non-progressive spermatozoa, compared to the conventional method. To investigate whether results drifted according to time of semen analysis, results were pooled into quarters according to date of semen analysis. CRISMAS motility results appeared more stable over time compared to the conventional analysis; however, neither method showed any trends. Apparently, CRISMAS CASA results and results from the conventional method were not comparable with respect to sperm concentration and motility analysis. This needs to be accounted for in clinics using this software and in studies of determinants of these semen characteristics.",
keywords = "Denmark, Diagnosis, Computer-Assisted, European Continental Ancestry Group, Humans, Image Processing, Computer-Assisted, Male, Semen Analysis, Software, Sperm Count, Sperm Motility, Young Adult",
author = "Anne Vested and Ramlau-Hansen, {Cecilia H} and Bonde, {Jens P} and Thulstrup, {Ane M} and Kristensen, {Susanne Lund} and Gunnar Toft",
year = "2011",
doi = "10.1038/aja.2011.14",
language = "English",
volume = "13",
pages = "453--8",
journal = "Asian Journal of Andrology",
issn = "1008-682X",
publisher = "Medknow Publications and Media Pvt. Ltd",
number = "3",

}

RIS

TY - JOUR

T1 - A comparison of conventional and computer-assisted semen analysis (CRISMAS software) using samples from 166 young Danish men

AU - Vested, Anne

AU - Ramlau-Hansen, Cecilia H

AU - Bonde, Jens P

AU - Thulstrup, Ane M

AU - Kristensen, Susanne Lund

AU - Toft, Gunnar

PY - 2011

Y1 - 2011

N2 - The aim of the present study was to compare assessments of sperm concentration and sperm motility analysed by conventional semen analysis with those obtained by computer-assisted semen analysis (CASA) (Copenhagen Rigshospitalet Image House Sperm Motility Analysis System (CRISMAS) 4.6 software) using semen samples from 166 young Danish men. The CRISMAS software identifies sperm concentration and classifies spermatozoa into three motility categories. To enable comparison of the two methods, the four motility stages obtained by conventional semen analysis were, based on their velocity classifications, divided into three stages, comparable to the three CRISMAS motility categories: rapidly progressive (A), slowly progressive (B) and non-progressive (C+D). Differences between the two methods were large for all investigated parameters (P <0.001). CRISMAS overestimated sperm concentration and the proportion of rapidly progressive spermatozoa and, consequently, underestimated the percentages of slowly progressive and non-progressive spermatozoa, compared to the conventional method. To investigate whether results drifted according to time of semen analysis, results were pooled into quarters according to date of semen analysis. CRISMAS motility results appeared more stable over time compared to the conventional analysis; however, neither method showed any trends. Apparently, CRISMAS CASA results and results from the conventional method were not comparable with respect to sperm concentration and motility analysis. This needs to be accounted for in clinics using this software and in studies of determinants of these semen characteristics.

AB - The aim of the present study was to compare assessments of sperm concentration and sperm motility analysed by conventional semen analysis with those obtained by computer-assisted semen analysis (CASA) (Copenhagen Rigshospitalet Image House Sperm Motility Analysis System (CRISMAS) 4.6 software) using semen samples from 166 young Danish men. The CRISMAS software identifies sperm concentration and classifies spermatozoa into three motility categories. To enable comparison of the two methods, the four motility stages obtained by conventional semen analysis were, based on their velocity classifications, divided into three stages, comparable to the three CRISMAS motility categories: rapidly progressive (A), slowly progressive (B) and non-progressive (C+D). Differences between the two methods were large for all investigated parameters (P <0.001). CRISMAS overestimated sperm concentration and the proportion of rapidly progressive spermatozoa and, consequently, underestimated the percentages of slowly progressive and non-progressive spermatozoa, compared to the conventional method. To investigate whether results drifted according to time of semen analysis, results were pooled into quarters according to date of semen analysis. CRISMAS motility results appeared more stable over time compared to the conventional analysis; however, neither method showed any trends. Apparently, CRISMAS CASA results and results from the conventional method were not comparable with respect to sperm concentration and motility analysis. This needs to be accounted for in clinics using this software and in studies of determinants of these semen characteristics.

KW - Denmark

KW - Diagnosis, Computer-Assisted

KW - European Continental Ancestry Group

KW - Humans

KW - Image Processing, Computer-Assisted

KW - Male

KW - Semen Analysis

KW - Software

KW - Sperm Count

KW - Sperm Motility

KW - Young Adult

U2 - 10.1038/aja.2011.14

DO - 10.1038/aja.2011.14

M3 - Journal article

C2 - 21516117

VL - 13

SP - 453

EP - 458

JO - Asian Journal of Andrology

JF - Asian Journal of Andrology

SN - 1008-682X

IS - 3

ER -