A comparative study of P450 gene expression in field and laboratory Musca domestica L. strains

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A comparative study of P450 gene expression in field and laboratory Musca domestica L. strains. / Højland, Dorte Heidi; Vagn Jensen, Karl-Martin ; Kristensen, Michael.

In: Pest Management Science, Vol. 70, No. 8, 2014, p. 1237-1242.

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@article{296142fe8b484756932c37ce986a8e45,
title = "A comparative study of P450 gene expression in field and laboratory Musca domestica L. strains",
abstract = "BACKGROUNDThe housefly is a global pest that has developed resistance to most insecticides applied for its control. Resistance has been associated with cytochrome P450 monooxygenases (P450s). The authors compare the expression of six genes possibly associated with insecticide resistance in three unselected strains: a multiresistant strain (791a), a neonicotinoid-resistant strain (766b) and a new field strain (845b).RESULTSCYP4G2 was highly expressed throughout the range of strains and proved to be the one of the most interesting expression profiles of all P450s analysed. CYP6G4 was expressed up to 11-fold higher in 766b than in WHO-SRS. Significant differences between expression of P450 genes between F1 flies from 845b and established laboratory strains were shown. In general, P450 gene expression in 845b was 2–14-fold higher than in the reference strain (P < 0.0101) and 2–23-fold higher than in the multiresistant strain (P < 0.0110).CONCLUSIONThe newly collected field strain 845b had significantly higher constitutive gene expression than both WHO-SRS and 791a. High constitutive expression of CYP4G2 in houseflies indicates a possible role of this gene in metabolic resistance. There is a strong indication that CYP6G4 is a major insecticide resistance gene involved in neonicotinoid resistance. {\circledC} 2013 Society of Chemical Industry",
author = "H{\o}jland, {Dorte Heidi} and {Vagn Jensen}, Karl-Martin and Michael Kristensen",
year = "2014",
doi = "10.1002/ps.3681",
language = "English",
volume = "70",
pages = "1237--1242",
journal = "Pest Management Science",
issn = "1526-498X",
publisher = "JohnWiley & Sons Ltd.",
number = "8",

}

RIS

TY - JOUR

T1 - A comparative study of P450 gene expression in field and laboratory Musca domestica L. strains

AU - Højland, Dorte Heidi

AU - Vagn Jensen, Karl-Martin

AU - Kristensen, Michael

PY - 2014

Y1 - 2014

N2 - BACKGROUNDThe housefly is a global pest that has developed resistance to most insecticides applied for its control. Resistance has been associated with cytochrome P450 monooxygenases (P450s). The authors compare the expression of six genes possibly associated with insecticide resistance in three unselected strains: a multiresistant strain (791a), a neonicotinoid-resistant strain (766b) and a new field strain (845b).RESULTSCYP4G2 was highly expressed throughout the range of strains and proved to be the one of the most interesting expression profiles of all P450s analysed. CYP6G4 was expressed up to 11-fold higher in 766b than in WHO-SRS. Significant differences between expression of P450 genes between F1 flies from 845b and established laboratory strains were shown. In general, P450 gene expression in 845b was 2–14-fold higher than in the reference strain (P < 0.0101) and 2–23-fold higher than in the multiresistant strain (P < 0.0110).CONCLUSIONThe newly collected field strain 845b had significantly higher constitutive gene expression than both WHO-SRS and 791a. High constitutive expression of CYP4G2 in houseflies indicates a possible role of this gene in metabolic resistance. There is a strong indication that CYP6G4 is a major insecticide resistance gene involved in neonicotinoid resistance. © 2013 Society of Chemical Industry

AB - BACKGROUNDThe housefly is a global pest that has developed resistance to most insecticides applied for its control. Resistance has been associated with cytochrome P450 monooxygenases (P450s). The authors compare the expression of six genes possibly associated with insecticide resistance in three unselected strains: a multiresistant strain (791a), a neonicotinoid-resistant strain (766b) and a new field strain (845b).RESULTSCYP4G2 was highly expressed throughout the range of strains and proved to be the one of the most interesting expression profiles of all P450s analysed. CYP6G4 was expressed up to 11-fold higher in 766b than in WHO-SRS. Significant differences between expression of P450 genes between F1 flies from 845b and established laboratory strains were shown. In general, P450 gene expression in 845b was 2–14-fold higher than in the reference strain (P < 0.0101) and 2–23-fold higher than in the multiresistant strain (P < 0.0110).CONCLUSIONThe newly collected field strain 845b had significantly higher constitutive gene expression than both WHO-SRS and 791a. High constitutive expression of CYP4G2 in houseflies indicates a possible role of this gene in metabolic resistance. There is a strong indication that CYP6G4 is a major insecticide resistance gene involved in neonicotinoid resistance. © 2013 Society of Chemical Industry

U2 - 10.1002/ps.3681

DO - 10.1002/ps.3681

M3 - Journal article

VL - 70

SP - 1237

EP - 1242

JO - Pest Management Science

JF - Pest Management Science

SN - 1526-498X

IS - 8

ER -