A combinatorial cell-laden gel microarray for inducing osteogenic differentiation of human mesenchymal stem cells

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Alireza Dolatshahi-Pirouz, Denmark
  • Mehdi Nikkhah, Harvard Univ, Harvard University, Sch Med, Brigham & Womens Hosp, Ctr Biomed Engn,Dept Med, United States
  • Akhilesh K. Gaharwar, Harvard Univ, Harvard University, Wyss Inst Biol Inspired Engn, Unknown
  • Basma Hashmi, Harvard Univ, Harvard University, Wyss Inst Biol Inspired Engn, Unknown
  • Enrico Guermani, Harvard Univ, Harvard University, Sch Med, Brigham & Womens Hosp, Ctr Biomed Engn,Dept Med, Unknown
  • Hamed Aliabadi, Harvard Univ, Harvard University, Sch Med, Brigham & Womens Hosp, Ctr Biomed Engn,Dept Med, Unknown
  • Gulden Camci-Unal, Harvard Univ, Harvard University, Sch Med, Brigham & Womens Hosp, Ctr Biomed Engn,Dept Med, Unknown
  • Thomas Ferrante, Harvard Univ, Harvard University, Wyss Inst Biol Inspired Engn, Unknown
  • Morten Foss
  • Donald E. Ingber, Harvard Univ, Harvard University, Wyss Inst Biol Inspired Engn, Unknown
  • Ali Khademhosseini, Harvard Univ, Harvard University, Wyss Inst Biol Inspired Engn, United States

Development of three dimensional (3D) microenvironments that direct stem cell differentiation into functional cell types remains a major challenge in the field of regenerative medicine. Here, we describe a new platform to address this challenge by utilizing a robotic microarray spotter for testing stem cell fates inside various miniaturized cell-laden gels in a systematic manner. To demonstrate the feasibility of our platform, we evaluated the osteogenic differentiation of human mesenchymal stem cells (hMSCs) within combinatorial 3D niches. We were able to identify specific combinations, that enhanced the expression of osteogenic markers. Notably, these 'hit' combinations directed hMSCs to form mineralized tissue when conditions were translated to 3D macroscale hydrogels, indicating that the miniaturization of the experimental system did not alter stem cell fate. Overall, our findings confirmed that the 3D cell-laden gel microarray can be used for screening of different conditions in a rapid, cost-effective, and multiplexed manner for a broad range of tissue engineering applications.

Original languageEnglish
Article number3896
JournalScientific Reports
Volume4
Number of pages9
ISSN2045-2322
DOIs
Publication statusPublished - 29 Jan 2014

    Research areas

  • 3D CULTURE-SYSTEMS, HIGH-THROUGHPUT, I COLLAGEN, FIBRONECTIN, ADHESION, FATE, MICROENVIRONMENT, BIOMATERIALS, EXPRESSION, PROTEINS

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