A bio-hybrid DNA rotor–stator nanoengine that moves along predefined tracks

Julian Valero Moreno; Nibedila Pal; Soma Dhakal; Nils G. Walter; Michael Famulok

doi:10.1038/s41565-018-0109-z

A bio-hybrid DNA rotor–stator nanoengine that moves along predefined tracks

Julian Valero Moreno, Nibedila Pal, Soma Dhakal, Nils G. Walter, Michael Famulok^*

^*Corresponding author for this work

Interdisciplinary Nanoscience Center - INANO-MBG, iNANO-huset

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

95 Citations (Scopus)

Abstract

Biological motors are highly complex protein assemblies that generate linear or rotary motion, powered by chemical energy. Synthetic motors based on DNA nanostructures, bio-hybrid designs or synthetic organic chemistry have been assembled. However, unidirectionally rotating biomimetic wheel motors with rotor–stator units that consume chemical energy are elusive. Here, we report a bio-hybrid nanoengine consisting of a catalytic stator that unidirectionally rotates an interlocked DNA wheel, powered by NTP hydrolysis. The engine consists of an engineered T7 RNA polymerase (T7RNAP-ZIF) attached to a dsDNA nanoring that is catenated to a rigid rotating dsDNA wheel. The wheel motor produces long, repetitive RNA transcripts that remain attached to the engine and are used to guide its movement along predefined ssDNA tracks arranged on a DNA nanotube. The simplicity of the design renders this walking nanoengine adaptable to other biological nanoarchitectures, facilitating the construction of complex bio-hybrid structures that achieve NTP-driven locomotion.

Original language	English
Journal	Nature Nanotechnology
Volume	13
Issue	6
Pages (from-to)	496-503
Number of pages	8
ISSN	1748-3387
DOIs	https://doi.org/10.1038/s41565-018-0109-z
Publication status	Published - 1 Jun 2018

Access to Document

10.1038/s41565-018-0109-z

Cite this

@article{1546969843e04536af5450b52fe243cf,

title = "A bio-hybrid DNA rotor–stator nanoengine that moves along predefined tracks",

abstract = "Biological motors are highly complex protein assemblies that generate linear or rotary motion, powered by chemical energy. Synthetic motors based on DNA nanostructures, bio-hybrid designs or synthetic organic chemistry have been assembled. However, unidirectionally rotating biomimetic wheel motors with rotor–stator units that consume chemical energy are elusive. Here, we report a bio-hybrid nanoengine consisting of a catalytic stator that unidirectionally rotates an interlocked DNA wheel, powered by NTP hydrolysis. The engine consists of an engineered T7 RNA polymerase (T7RNAP-ZIF) attached to a dsDNA nanoring that is catenated to a rigid rotating dsDNA wheel. The wheel motor produces long, repetitive RNA transcripts that remain attached to the engine and are used to guide its movement along predefined ssDNA tracks arranged on a DNA nanotube. The simplicity of the design renders this walking nanoengine adaptable to other biological nanoarchitectures, facilitating the construction of complex bio-hybrid structures that achieve NTP-driven locomotion.",

author = "{Valero Moreno}, Julian and Nibedila Pal and Soma Dhakal and Walter, {Nils G.} and Michael Famulok",

year = "2018",

month = jun,

day = "1",

doi = "10.1038/s41565-018-0109-z",

language = "English",

volume = "13",

pages = "496--503",

journal = "Nature Nanotechnology",

issn = "1748-3387",

publisher = "Nature Publishing Group",

number = "6",

}

TY - JOUR

T1 - A bio-hybrid DNA rotor–stator nanoengine that moves along predefined tracks

AU - Valero Moreno, Julian

AU - Pal, Nibedila

AU - Dhakal, Soma

AU - Walter, Nils G.

AU - Famulok, Michael

PY - 2018/6/1

Y1 - 2018/6/1

N2 - Biological motors are highly complex protein assemblies that generate linear or rotary motion, powered by chemical energy. Synthetic motors based on DNA nanostructures, bio-hybrid designs or synthetic organic chemistry have been assembled. However, unidirectionally rotating biomimetic wheel motors with rotor–stator units that consume chemical energy are elusive. Here, we report a bio-hybrid nanoengine consisting of a catalytic stator that unidirectionally rotates an interlocked DNA wheel, powered by NTP hydrolysis. The engine consists of an engineered T7 RNA polymerase (T7RNAP-ZIF) attached to a dsDNA nanoring that is catenated to a rigid rotating dsDNA wheel. The wheel motor produces long, repetitive RNA transcripts that remain attached to the engine and are used to guide its movement along predefined ssDNA tracks arranged on a DNA nanotube. The simplicity of the design renders this walking nanoengine adaptable to other biological nanoarchitectures, facilitating the construction of complex bio-hybrid structures that achieve NTP-driven locomotion.

AB - Biological motors are highly complex protein assemblies that generate linear or rotary motion, powered by chemical energy. Synthetic motors based on DNA nanostructures, bio-hybrid designs or synthetic organic chemistry have been assembled. However, unidirectionally rotating biomimetic wheel motors with rotor–stator units that consume chemical energy are elusive. Here, we report a bio-hybrid nanoengine consisting of a catalytic stator that unidirectionally rotates an interlocked DNA wheel, powered by NTP hydrolysis. The engine consists of an engineered T7 RNA polymerase (T7RNAP-ZIF) attached to a dsDNA nanoring that is catenated to a rigid rotating dsDNA wheel. The wheel motor produces long, repetitive RNA transcripts that remain attached to the engine and are used to guide its movement along predefined ssDNA tracks arranged on a DNA nanotube. The simplicity of the design renders this walking nanoengine adaptable to other biological nanoarchitectures, facilitating the construction of complex bio-hybrid structures that achieve NTP-driven locomotion.

UR - http://www.scopus.com/inward/record.url?scp=85045112544&partnerID=8YFLogxK

U2 - 10.1038/s41565-018-0109-z

DO - 10.1038/s41565-018-0109-z

M3 - Journal article

SN - 1748-3387

VL - 13

SP - 496

EP - 503

JO - Nature Nanotechnology

JF - Nature Nanotechnology

IS - 6

ER -

A bio-hybrid DNA rotor–stator nanoengine that moves along predefined tracks

Abstract

Access to Document

Other files and links

Fingerprint

Cite this