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8-methoxycaffeine inhibition of Drosophila DNA topoisomerase II

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  • S Tornaletti, Istituto di Genetica Biochimica ed Evoluzionistica del C.N.R., Pavia, Italy.
  • ,
  • A H Andersen
  • K Christiansen
  • ,
  • A M Pedrini, Istituto di Genetica Biochimica ed Evoluzionistica del C.N.R., Pavia, Italy.

We have investigated the effect of 8-methoxycaffeine on the interaction between Drosophila DNA topoisomerase II and DNA. We have shown that 8-methoxycaffeine affected the enzyme strand-passing activity by inhibiting decatenation of kinetoplast DNA, and that it interfered with the breakage-reunion reaction by stabilizing a cleavable complex. Treatment of the cleavable complex with protein denaturant resulted in DNA breaks. High resolution mapping of the cleavage sites in the central spacer region of Tetrahymena rDNA revealed that, contrary to what was observed with clinically important DNA topoisomerase II inhibitors, 8-methoxycaffeine did not modify the cleavage pattern observed without the drug.

Original languageEnglish
JournalB B A - General Subjects
Volume1131
Issue1
Pages (from-to)30-3
Number of pages4
ISSN0006-3002
Publication statusPublished - 7 May 1992

    Research areas

  • Animals, Binding Sites, Caffeine, Drosophila, Topoisomerase II Inhibitors, Journal Article, Research Support, Non-U.S. Gov't

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