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2'3'-cGAMP triggers a STING- and NF-κB-dependent broad antiviral response in Drosophila

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2'3'-cGAMP triggers a STING- and NF-κB-dependent broad antiviral response in Drosophila. / Cai, Hua; Holleufer, Andreas; Simonsen, Bine et al.

In: Science Signaling, Vol. 13, No. 660, eabc4537, 12.2020.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Cai, H, Holleufer, A, Simonsen, B, Schneider, J, Lemoine, A, Gad, HH, Huang, J, Huang, J, Chen, D, Peng, T, Marques, JT, Hartmann, R, Martins, NE & Imler, JL 2020, '2'3'-cGAMP triggers a STING- and NF-κB-dependent broad antiviral response in Drosophila', Science Signaling, vol. 13, no. 660, eabc4537. https://doi.org/10.1126/scisignal.abc4537

APA

Cai, H., Holleufer, A., Simonsen, B., Schneider, J., Lemoine, A., Gad, H. H., Huang, J., Huang, J., Chen, D., Peng, T., Marques, J. T., Hartmann, R., Martins, N. E., & Imler, J. L. (2020). 2'3'-cGAMP triggers a STING- and NF-κB-dependent broad antiviral response in Drosophila. Science Signaling, 13(660), [eabc4537]. https://doi.org/10.1126/scisignal.abc4537

CBE

Cai H, Holleufer A, Simonsen B, Schneider J, Lemoine A, Gad HH, Huang J, Huang J, Chen D, Peng T, et al. 2020. 2'3'-cGAMP triggers a STING- and NF-κB-dependent broad antiviral response in Drosophila. Science Signaling. 13(660):Article eabc4537. https://doi.org/10.1126/scisignal.abc4537

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Bibtex

@article{acb22c9b4417412c94fc9e812ca76554,
title = "2'3'-cGAMP triggers a STING- and NF-κB-dependent broad antiviral response in Drosophila",
abstract = "We previously reported that an ortholog of STING regulates infection by picorna-like viruses in Drosophila In mammals, STING is activated by the cyclic dinucleotide 2'3'-cGAMP produced by cGAS, which acts as a receptor for cytosolic DNA. Here, we showed that injection of flies with 2'3'-cGAMP induced the expression of dSTING-regulated genes. Coinjection of 2'3'-cGAMP with a panel of RNA or DNA viruses resulted in substantially reduced viral replication. This 2'3'-cGAMP-mediated protection was still observed in flies with mutations in Atg7 and AGO2, genes that encode key components of the autophagy and small interfering RNA pathways, respectively. By contrast, this protection was abrogated in flies with mutations in the gene encoding the NF-κB transcription factor Relish. Transcriptomic analysis of 2'3'-cGAMP-injected flies revealed a complex response pattern in which genes were rapidly induced, induced after a delay, or induced in a sustained manner. Our results reveal that dSTING regulates an NF-κB-dependent antiviral program that predates the emergence of interferons in vertebrates.",
author = "Hua Cai and Andreas Holleufer and Bine Simonsen and Juliette Schneider and Aur{\'e}lie Lemoine and Gad, {Hans Henrik} and Jingxian Huang and Jieqing Huang and Di Chen and Tao Peng and Marques, {Jo{\~a}o T.} and Rune Hartmann and Martins, {Nelson E.} and Imler, {Jean Luc}",
year = "2020",
month = dec,
doi = "10.1126/scisignal.abc4537",
language = "English",
volume = "13",
journal = "Science Signaling",
issn = "1945-0877",
publisher = "American Association for the Advancement of Science",
number = "660",

}

RIS

TY - JOUR

T1 - 2'3'-cGAMP triggers a STING- and NF-κB-dependent broad antiviral response in Drosophila

AU - Cai, Hua

AU - Holleufer, Andreas

AU - Simonsen, Bine

AU - Schneider, Juliette

AU - Lemoine, Aurélie

AU - Gad, Hans Henrik

AU - Huang, Jingxian

AU - Huang, Jieqing

AU - Chen, Di

AU - Peng, Tao

AU - Marques, João T.

AU - Hartmann, Rune

AU - Martins, Nelson E.

AU - Imler, Jean Luc

PY - 2020/12

Y1 - 2020/12

N2 - We previously reported that an ortholog of STING regulates infection by picorna-like viruses in Drosophila In mammals, STING is activated by the cyclic dinucleotide 2'3'-cGAMP produced by cGAS, which acts as a receptor for cytosolic DNA. Here, we showed that injection of flies with 2'3'-cGAMP induced the expression of dSTING-regulated genes. Coinjection of 2'3'-cGAMP with a panel of RNA or DNA viruses resulted in substantially reduced viral replication. This 2'3'-cGAMP-mediated protection was still observed in flies with mutations in Atg7 and AGO2, genes that encode key components of the autophagy and small interfering RNA pathways, respectively. By contrast, this protection was abrogated in flies with mutations in the gene encoding the NF-κB transcription factor Relish. Transcriptomic analysis of 2'3'-cGAMP-injected flies revealed a complex response pattern in which genes were rapidly induced, induced after a delay, or induced in a sustained manner. Our results reveal that dSTING regulates an NF-κB-dependent antiviral program that predates the emergence of interferons in vertebrates.

AB - We previously reported that an ortholog of STING regulates infection by picorna-like viruses in Drosophila In mammals, STING is activated by the cyclic dinucleotide 2'3'-cGAMP produced by cGAS, which acts as a receptor for cytosolic DNA. Here, we showed that injection of flies with 2'3'-cGAMP induced the expression of dSTING-regulated genes. Coinjection of 2'3'-cGAMP with a panel of RNA or DNA viruses resulted in substantially reduced viral replication. This 2'3'-cGAMP-mediated protection was still observed in flies with mutations in Atg7 and AGO2, genes that encode key components of the autophagy and small interfering RNA pathways, respectively. By contrast, this protection was abrogated in flies with mutations in the gene encoding the NF-κB transcription factor Relish. Transcriptomic analysis of 2'3'-cGAMP-injected flies revealed a complex response pattern in which genes were rapidly induced, induced after a delay, or induced in a sustained manner. Our results reveal that dSTING regulates an NF-κB-dependent antiviral program that predates the emergence of interferons in vertebrates.

UR - http://www.scopus.com/inward/record.url?scp=85097036435&partnerID=8YFLogxK

U2 - 10.1126/scisignal.abc4537

DO - 10.1126/scisignal.abc4537

M3 - Journal article

C2 - 33262294

AN - SCOPUS:85097036435

VL - 13

JO - Science Signaling

JF - Science Signaling

SN - 1945-0877

IS - 660

M1 - eabc4537

ER -