σ1B adaptin regulates adipogenesis by mediating the sorting of sortilin in adipose tissue

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σ1B adaptin regulates adipogenesis by mediating the sorting of sortilin in adipose tissue. / Baltes, Jennifer; Larsen, Jakob Vejby; Radhakrishnan, Karthikeyan; Geumann, Constanze; Kratzke, Manuel; Petersen, Claus Munck; Schu, Peter.

In: Journal of Cell Science, Vol. 127, No. Pt 16, 15.08.2014, p. 3477-87.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Baltes, J, Larsen, JV, Radhakrishnan, K, Geumann, C, Kratzke, M, Petersen, CM & Schu, P 2014, 'σ1B adaptin regulates adipogenesis by mediating the sorting of sortilin in adipose tissue', Journal of Cell Science, vol. 127, no. Pt 16, pp. 3477-87. https://doi.org/10.1242/jcs.146886

APA

Baltes, J., Larsen, J. V., Radhakrishnan, K., Geumann, C., Kratzke, M., Petersen, C. M., & Schu, P. (2014). σ1B adaptin regulates adipogenesis by mediating the sorting of sortilin in adipose tissue. Journal of Cell Science, 127(Pt 16), 3477-87. https://doi.org/10.1242/jcs.146886

CBE

Baltes J, Larsen JV, Radhakrishnan K, Geumann C, Kratzke M, Petersen CM, Schu P. 2014. σ1B adaptin regulates adipogenesis by mediating the sorting of sortilin in adipose tissue. Journal of Cell Science. 127(Pt 16):3477-87. https://doi.org/10.1242/jcs.146886

MLA

Vancouver

Baltes J, Larsen JV, Radhakrishnan K, Geumann C, Kratzke M, Petersen CM et al. σ1B adaptin regulates adipogenesis by mediating the sorting of sortilin in adipose tissue. Journal of Cell Science. 2014 Aug 15;127(Pt 16):3477-87. https://doi.org/10.1242/jcs.146886

Author

Baltes, Jennifer ; Larsen, Jakob Vejby ; Radhakrishnan, Karthikeyan ; Geumann, Constanze ; Kratzke, Manuel ; Petersen, Claus Munck ; Schu, Peter. / σ1B adaptin regulates adipogenesis by mediating the sorting of sortilin in adipose tissue. In: Journal of Cell Science. 2014 ; Vol. 127, No. Pt 16. pp. 3477-87.

Bibtex

@article{855ee966c69243799ece4ead2021b229,
title = "σ1B adaptin regulates adipogenesis by mediating the sorting of sortilin in adipose tissue",
abstract = "Here, we describe altered sorting of sortilin in adipocytes deficient for the σ1B-containing AP-1 complex, leading to the inhibition of adipogenesis. The AP-1 complex mediates protein sorting between the trans-Golgi network and endosomes. Vertebrates express three AP1 σ1 subunit isoforms - σ1A, σ1B and σ1C (also known as AP1S1, AP1S2 and AP1S3, respectively). σ1B-deficient mice display impaired recycling of synaptic vesicles and lipodystrophy. Here, we show that sortilin is overexpressed in adipose tissue from σ1B(-/-) mice, and that its overexpression in wild-type cells is sufficient to suppress adipogenesis. σ1B-specific binding of sortilin requires the sortilin DxxD-x12-DSxxxL motif. σ1B deficiency does not lead to a block of sortilin transport out of a specific organelle, but the fraction that reaches lysosomes is reduced. Sortilin binds to the receptor DLK1, an inhibitor of adipocyte differentiation, and the overexpression of sortilin prevents DLK1 downregulation, leading to enhanced inhibition of adipogenesis. DLK1 and sortilin expression are not increased in the brain tissue of σ1B(-/-) mice, although this is the tissue with the highest expression of σ1B and sortilin. Thus, adipose-tissue-specific and σ1B-dependent routes for the transport of sortilin exist and are involved in the regulation of adipogenesis and adipose-tissue mass.",
author = "Jennifer Baltes and Larsen, {Jakob Vejby} and Karthikeyan Radhakrishnan and Constanze Geumann and Manuel Kratzke and Petersen, {Claus Munck} and Peter Schu",
note = "{\textcopyright} 2014. Published by The Company of Biologists Ltd.",
year = "2014",
month = aug,
day = "15",
doi = "10.1242/jcs.146886",
language = "English",
volume = "127",
pages = "3477--87",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "The/Company of Biologists Ltd.",
number = "Pt 16",

}

RIS

TY - JOUR

T1 - σ1B adaptin regulates adipogenesis by mediating the sorting of sortilin in adipose tissue

AU - Baltes, Jennifer

AU - Larsen, Jakob Vejby

AU - Radhakrishnan, Karthikeyan

AU - Geumann, Constanze

AU - Kratzke, Manuel

AU - Petersen, Claus Munck

AU - Schu, Peter

N1 - © 2014. Published by The Company of Biologists Ltd.

PY - 2014/8/15

Y1 - 2014/8/15

N2 - Here, we describe altered sorting of sortilin in adipocytes deficient for the σ1B-containing AP-1 complex, leading to the inhibition of adipogenesis. The AP-1 complex mediates protein sorting between the trans-Golgi network and endosomes. Vertebrates express three AP1 σ1 subunit isoforms - σ1A, σ1B and σ1C (also known as AP1S1, AP1S2 and AP1S3, respectively). σ1B-deficient mice display impaired recycling of synaptic vesicles and lipodystrophy. Here, we show that sortilin is overexpressed in adipose tissue from σ1B(-/-) mice, and that its overexpression in wild-type cells is sufficient to suppress adipogenesis. σ1B-specific binding of sortilin requires the sortilin DxxD-x12-DSxxxL motif. σ1B deficiency does not lead to a block of sortilin transport out of a specific organelle, but the fraction that reaches lysosomes is reduced. Sortilin binds to the receptor DLK1, an inhibitor of adipocyte differentiation, and the overexpression of sortilin prevents DLK1 downregulation, leading to enhanced inhibition of adipogenesis. DLK1 and sortilin expression are not increased in the brain tissue of σ1B(-/-) mice, although this is the tissue with the highest expression of σ1B and sortilin. Thus, adipose-tissue-specific and σ1B-dependent routes for the transport of sortilin exist and are involved in the regulation of adipogenesis and adipose-tissue mass.

AB - Here, we describe altered sorting of sortilin in adipocytes deficient for the σ1B-containing AP-1 complex, leading to the inhibition of adipogenesis. The AP-1 complex mediates protein sorting between the trans-Golgi network and endosomes. Vertebrates express three AP1 σ1 subunit isoforms - σ1A, σ1B and σ1C (also known as AP1S1, AP1S2 and AP1S3, respectively). σ1B-deficient mice display impaired recycling of synaptic vesicles and lipodystrophy. Here, we show that sortilin is overexpressed in adipose tissue from σ1B(-/-) mice, and that its overexpression in wild-type cells is sufficient to suppress adipogenesis. σ1B-specific binding of sortilin requires the sortilin DxxD-x12-DSxxxL motif. σ1B deficiency does not lead to a block of sortilin transport out of a specific organelle, but the fraction that reaches lysosomes is reduced. Sortilin binds to the receptor DLK1, an inhibitor of adipocyte differentiation, and the overexpression of sortilin prevents DLK1 downregulation, leading to enhanced inhibition of adipogenesis. DLK1 and sortilin expression are not increased in the brain tissue of σ1B(-/-) mice, although this is the tissue with the highest expression of σ1B and sortilin. Thus, adipose-tissue-specific and σ1B-dependent routes for the transport of sortilin exist and are involved in the regulation of adipogenesis and adipose-tissue mass.

U2 - 10.1242/jcs.146886

DO - 10.1242/jcs.146886

M3 - Journal article

C2 - 24928897

VL - 127

SP - 3477

EP - 3487

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - Pt 16

ER -