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100% complete assignment of non-labile (1)H, (13)C, and (15)N signals for calcium-loaded Calbindin D(9k) P43G

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  • Nur Alia Oktaviani, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands.
  • ,
  • Renee Otten, University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute, Netherlands
  • Klaas Dijkstra, University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute, Netherlands
  • Ruud M Scheek, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Netherlands
  • Eva Thulin, Lund University, Sweden
  • Mikael Akke, Lund University, Sweden
  • Frans A A Mulder

Here we present the 100% complete assignment chemical shift of non-labile (1)H, (15)N and (13)C nuclei of Calbindin D(9k) P43G. The assignment includes all non-exchangeable side chain nuclei, including ones that are rarely reported, such as LysNζ as well as the termini. NMR experiments required to achieve truly complete assignments are discussed. To the best of our knowledge our assignments for Calbindin D(9k) extend beyond previous studies reaching near-completeness (Vis et al. in Biochem 33:14858-14870, 1994; Yamazaki et al. in J Am Chem Soc 116:6464-6465, 1994; Yamazaki et al. in Biochem 32:5656-5669, 1993b).

Original languageEnglish
JournalBiomolecular N M R Assignments
Volume5
Issue1
Pages (from-to)79-84
Number of pages6
ISSN1874-2718
DOIs
Publication statusPublished - Apr 2011
Externally publishedYes

    Research areas

  • Amino Acid Sequence, Amino Acid Substitution, Animals, Calbindins, Calcium, Carbon Isotopes, Cattle, Hydrogen, Molecular Sequence Data, Nitrogen Isotopes, Nuclear Magnetic Resonance, Biomolecular, S100 Calcium Binding Protein G

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