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Yuya Hayashi

Time-course profiling of molecular stress responses to silver nanoparticles in the earthworm Eisenia fetida

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Time-course profiling of molecular stress responses to silver nanoparticles in the earthworm Eisenia fetida. / Hayashi, Yuya; Heckmann, Lars-Henrik; Simonsen, Vibeke; Scott-Fordsmand, Janeck James.

In: Ecotoxicology and Environmental Safety, Vol. 98, 2013, p. 219-226.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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Hayashi, Yuya ; Heckmann, Lars-Henrik ; Simonsen, Vibeke ; Scott-Fordsmand, Janeck James. / Time-course profiling of molecular stress responses to silver nanoparticles in the earthworm Eisenia fetida. In: Ecotoxicology and Environmental Safety. 2013 ; Vol. 98. pp. 219-226.

Bibtex

@article{23642507ad4d410e8385ab5b3e6c9666,
title = "Time-course profiling of molecular stress responses to silver nanoparticles in the earthworm Eisenia fetida",
abstract = "The molecular mechanism of silver nanoparticle (AgNP) toxicity, particularly its temporal aspect, is currently limited in the literature. This study seeks to identify and profile changes in molecular response patterns over time during soil exposure of the earthworm Eisenia fetida to AgNPs (82±27 nm) with reference to dissolved silver salt (AgNO3). Principal component analysis of selected gene and enzyme response profiles revealed dissimilar patterns between AgNO3 and AgNP treatments and also over time. Despite the observed difference in molecular profiles, the body burdens of total Ag were within the same range (10–40 mg/kg dry weight worm) for both treatments with apparent correlation to the induction pattern of metallothionein. AgNO3 induced the genes and enzymes related to oxidative stress at day 1, after which markers of energy metabolism were all suppressed at day 2. Exposure to AgNPs likewise led to induction of oxidative stress genes at day 2, but with a temporal pattern shift to immune genes at day 14 following metabolic upregulation at day 7. The involvement of oxidative stress and subsequent alterations in immune gene regulation were as predicted by our in vitro study reported previously, highlighting the importance of immunological endpoints in nanosilver toxicity.",
author = "Yuya Hayashi and Lars-Henrik Heckmann and Vibeke Simonsen and Scott-Fordsmand, {Janeck James}",
year = "2013",
doi = "10.1016/j.ecoenv.2013.08.017",
language = "English",
volume = "98",
pages = "219--226",
journal = "Ecotoxicology and Environmental Safety",
issn = "0147-6513",
publisher = "Academic Press",

}

RIS

TY - JOUR

T1 - Time-course profiling of molecular stress responses to silver nanoparticles in the earthworm Eisenia fetida

AU - Hayashi, Yuya

AU - Heckmann, Lars-Henrik

AU - Simonsen, Vibeke

AU - Scott-Fordsmand, Janeck James

PY - 2013

Y1 - 2013

N2 - The molecular mechanism of silver nanoparticle (AgNP) toxicity, particularly its temporal aspect, is currently limited in the literature. This study seeks to identify and profile changes in molecular response patterns over time during soil exposure of the earthworm Eisenia fetida to AgNPs (82±27 nm) with reference to dissolved silver salt (AgNO3). Principal component analysis of selected gene and enzyme response profiles revealed dissimilar patterns between AgNO3 and AgNP treatments and also over time. Despite the observed difference in molecular profiles, the body burdens of total Ag were within the same range (10–40 mg/kg dry weight worm) for both treatments with apparent correlation to the induction pattern of metallothionein. AgNO3 induced the genes and enzymes related to oxidative stress at day 1, after which markers of energy metabolism were all suppressed at day 2. Exposure to AgNPs likewise led to induction of oxidative stress genes at day 2, but with a temporal pattern shift to immune genes at day 14 following metabolic upregulation at day 7. The involvement of oxidative stress and subsequent alterations in immune gene regulation were as predicted by our in vitro study reported previously, highlighting the importance of immunological endpoints in nanosilver toxicity.

AB - The molecular mechanism of silver nanoparticle (AgNP) toxicity, particularly its temporal aspect, is currently limited in the literature. This study seeks to identify and profile changes in molecular response patterns over time during soil exposure of the earthworm Eisenia fetida to AgNPs (82±27 nm) with reference to dissolved silver salt (AgNO3). Principal component analysis of selected gene and enzyme response profiles revealed dissimilar patterns between AgNO3 and AgNP treatments and also over time. Despite the observed difference in molecular profiles, the body burdens of total Ag were within the same range (10–40 mg/kg dry weight worm) for both treatments with apparent correlation to the induction pattern of metallothionein. AgNO3 induced the genes and enzymes related to oxidative stress at day 1, after which markers of energy metabolism were all suppressed at day 2. Exposure to AgNPs likewise led to induction of oxidative stress genes at day 2, but with a temporal pattern shift to immune genes at day 14 following metabolic upregulation at day 7. The involvement of oxidative stress and subsequent alterations in immune gene regulation were as predicted by our in vitro study reported previously, highlighting the importance of immunological endpoints in nanosilver toxicity.

U2 - 10.1016/j.ecoenv.2013.08.017

DO - 10.1016/j.ecoenv.2013.08.017

M3 - Journal article

C2 - 24041528

VL - 98

SP - 219

EP - 226

JO - Ecotoxicology and Environmental Safety

JF - Ecotoxicology and Environmental Safety

SN - 0147-6513

ER -