Yonglun Luo

PhD, Associate Professor, Associate professor

Profile photo

Yonglun Luo

Associate professor

  • Department of Biomedicine - Forskning og uddannelse, Skou-bygningen
Postal address:
Høegh-Guldbergs Gade 10
1116, 152
8000
Aarhus C
Denmark

Email: alun@biomed.au.dk

Mobile: +4522411944

Phone: +4522411944

Curriculum Vitae

2003.9 -2006.7 China Agricultural University, molecular biology and biochemistry, Bachelor

2006.9-2009.7 Chinese Academy of Sciences, behavioral genetics, Master

2008.12-2011.11 Aarhus University, PhD in Medicine

2011.12-2012.03 Research assistant, Department of Biomedicine, Aarhus University 

2012.04-2012.07 Postdoc, Department of Biomedicine, Aarhus University

2012.07-2014.06 STAR postdoc, Novo Nordisk A/S; Department of Biomedicine, Aarhus University

Employment

Associate professor

Department of Biomedicine - Forskning og uddannelse, Skou-bygningen

Aarhus University

Aarhus C, Denmark

1 May 2018 → present

Associate professor

Department of Biomedicine - Forskning og uddannelse, Øst

Aarhus University

Aarhus C, Denmark

21 Oct 20171 May 2018

Department of Biomedicine - Forskning og uddannelse, Øst

Aarhus University

Aarhus C, Denmark

11 Nov 20146 Feb 2018

Department of Biomedicine - Forskning og uddannelse, Øst

Aarhus University

Aarhus C, Denmark

2 Jun 20173 Oct 2017

Department of Biomedicine - Forskning og uddannelse, Øst

Aarhus University

Aarhus C, Denmark

8 Jun 201411 Nov 2014

Department of Biomedicine - Forskning og uddannelse, Øst

Aarhus University

Aarhus C, Denmark

9 May 20148 Jun 2014

Department of Biomedicine - Forskning og uddannelse, Øst

Aarhus University

Aarhus C, Denmark

14 Apr 20149 May 2014

Department of Biomedicine - Forskning og uddannelse, Øst

Aarhus University

Aarhus C, Denmark

17 Dec 201311 Apr 2014

Department of Biomedicine - Department of Human Genetics

Aarhus University

Aarhus C, Denmark

29 Jul 201117 Dec 2013

Exchange Student

Department of Human Genetics

Aarhus University

Aarhus C, Denmark

15 Jul 200830 Nov 2008

Publications

The metabolic engine of endothelial cells

Falkenberg, K. D., Rohlenova, K., Luo, Y. & Carmeliet, P., Oct 2019, In : Nature Metabolism.

Single-Cell Transcriptome Analysis of Uniparental Embryos Reveals Parent-of-Origin Effects on Human Preimplantation Development

Leng, L., Sun, J., Huang, J., Gong, F., Yang, L., Zhang, S., Yuan, X., Fang, F., Xu, X., Luo, Y., Bolund, L., Peters, B. A., Lu, G., Jiang, T., Xu, F. & Lin, G., 27 Sep 2019, In : Cell Stem Cell.

Population Genomics of an Obligately Halophilic Basidiomycete Wallemia ichthyophaga

Gostinčar, C., Sun, X., Zajc, J., Fang, C., Hou, Y., Luo, Y., Gunde-Cimerman, N. & Song, Z., Sep 2019, In : Frontiers in Microbiology.

Refining CRISPR-based genome and epigenome editing off-targets

Luo, Y., 22 Jun 2019, In : Cell Biology and Toxicology.

Double-strand breaks in ribosomal RNA genes activate a distinct signaling and chromatin response to facilitate nucleolar restructuring and repair

Korsholm, L. M., Gál, Z., Lin, L., Quevedo, O., Ahmad, D. A., Dulina, E., Luo, Y., Bartek, J. & Larsen, D. H., 11 Jun 2019, In : Nucleic Acids Research.

LION: a simple and rapid method to achieve CRISPR gene editing

Xiang, X., Luo, L., Nodzyński, M., Li, C., Han, P., Dou, H., Petersen, T. S., Liang, X., Pan, X., Qu, K., Yang, L., Dang, Y., Liu, X., Bolund, L., Zhang, X., Tong, G., Xing, Y., Luo, Y. & Lin, L., 18 Mar 2019, In : Cellular and molecular life sciences : CMLS.

An overview and metanalysis of Machine and Deep Learning-Based CRISPR gRNA Design Tools

Wang, J., Zhang, X., Cheng, L. & Luo, Y., 2019, In : RNA Biology. 11 p.

CRISPR/Cas9-Mediated Gene Tagging: A Step-by-Step Protocol

Xiang, X., Li, C., Chen, X., Dou, H., Li, Y., Zhang, X. & Luo, Y., 2019, CRISPR gene editing. Luo, Y. (ed.). New York: Humana Press, p. 255-269 15 p. (Methods in Molecular Biology, Vol. 1961).

Functional Evaluation of CRISPR Activity by the Dual-Fluorescent Surrogate System: C-Check

Lin, L. & Luo, Y., 2019, CRISPR Gene Editing: Methods and Protocols. Luo, Y. (ed.). Springer Science+Business Media, p. 67-77 11 p. (Methods in Molecular Biology, Vol. 1961).

Rapid and Efficient Gene Deletion by CRISPR/Cas9

Neldeborg, S., Lin, L., Stougaard, M. & Luo, Y., 2019, CRISPR Gene Editing: Methods and Protocols. Humana Press, p. 233-247 15 p. (Methods in Molecular Biology, Vol. 1961).

Tracking CRISPR's Footprints

Lin, L. & Luo, Y., 2019, CRISPR Gene Editing: Methods and Protocols. Luo, Y. (ed.). Springer Science+Business Media, p. 13-28 16 p. (Methods in Molecular Biology, Vol. 1961).

CRISPR-C: circularization of genes and chromosome by CRISPR in human cells

Møller, H. D., Lin, L., Xiang, X., Petersen, T. S., Huang, J., Yang, L., Kjeldsen, E., Jensen, U. B., Zhang, X., Liu, X., Xu, X., Wang, J., Yang, H., Church, G. M., Bolund, L., Regenberg, B. & Luo, Y., 14 Dec 2018, In : Nucleic Acids Research. 46, 22, p. e131

STAT3 associates with vacuolar H+-ATPase and regulates cytosolic and lysosomal pH

Liu, B., Palmfeldt, J., Lin, L., Colaço, A., Clemmensen, K. K. B., Huang, J., Xu, F., Liu, X., Maeda, K., Luo, Y. & Jäättelä, M., Oct 2018, In : Cell Research. 28, 10, p. 996–1012 17 p.

Nrf2 negatively regulates STING indicating a link between antiviral sensing and metabolic reprogramming

Olagnier, D., Brandtoft, A. M., Gunderstofte, C., Villadsen, N. L., Krapp, C., Thielke, A. L., Laustsen, A., Peri, S., Hansen, A. L., Bonefeld, L., Thyrsted, J., Bruun, V., Iversen, M. B., Lin, L., Artegoitia, V. M., Su, C., Yang, L., Lin, R., Balachandran, S., Luo, Y., Nyegaard, M., Marrero, B., Goldbach-Mansky, R., Motwani, M., Ryan, D. G., Fitzgerald, K. A., O'Neill, L. A., Hollensen, A. K., Damgaard, C. K., de Paoli, F. V., Bertram, H. C., Jakobsen, M. R., Poulsen, T. B. & Holm, C. K., 29 Aug 2018, In : Nature Communications. 9, 1, 3506.

Simple method for assembly of CRISPR synergistic activation mediator gRNA expression array

Vad-Nielsen, J., Nielsen, A. L. & Luo, Y., 20 May 2018, In : Journal of Biotechnology. 274, p. 54-57 4 p.

Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases

Lin, L., Liu, Y., Xu, F., Huang, J., Daugaard, T. F., Petersen, T. S., Hansen, B., Ye, L., Zhou, Q., Fang, F., Yang, L., Li, S., Fløe, L., Jensen, K. T., Shrock, E., Chen, F., Yang, H., Wang, J., Liu, X., Xu, X., Bolund, L., Nielsen, A. L. & Luo, Y., 1 Mar 2018, In : GigaScience. 7, 3, p. 1-19 19 p.

Central and peripheral nervous system progenitors derived from human pluripotent stem cells reveal a unique temporal and cell-type specific expression of PMCAs

Chen, M., Laursen, S. H., Habekost, M., Knudsen, C. H., Buchholdt, S. H., Huang, J., Xu, F., Liu, X., Bolund, L., Luo, Y., Nissen, P., Febbraro, F. & Denham, M., 6 Feb 2018, In : Frontiers in Cell and Developmental Biology. 6, 10 p., 5.

Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9

Niu, D., Wei, H-J., Lin, L., George, H., Wang, T., Lee, I-H., Zhao, H-Y., Kan, Y., Shrock, E., Lesha, E., Wang, G., Luo, Y., Qing, Y., Jiao, D., Zhao, H., Zhou, X., Wang, S., Wei, H., Güell, M., Church, G. M. & Yang, L., 22 Sep 2017, In : Science. 357, 6357, p. 1303-1307 5 p.

Chromatin accessibility and guide sequence secondary structure affect CRISPR-Cas9 gene editing efficiency

Jensen, K. T., Fløe, L., Petersen, T. S., Huang, J., Xu, F., Bolund, L., Luo, Y. & Lin, L., 5 Jun 2017, In : FEBS Letters. 591, 13, p. 1892-1901 10 p.

RNA-Guided Activation of Pluripotency Genes in Human Fibroblasts

Xiong, K., Zhou, Y., Blichfeld, K. A., Hyttel, P., Bolund, L., Freude, K. K. & Luo, Y., Jun 2017, In : Cellular reprogramming. 19, 3, p. 189-198 10 p.

IGF1R depletion facilitates MET-amplification as mechanism of acquired resistance to erlotinib in HCC827 NSCLC cells

Hussmann, D., Madsen, A. T., Jakobsen, K. R., Luo, Y., Sorensen, B. S. & Nielsen, A. L., 18 Mar 2017, In : OncoTarget. 8

Induced pluripotent stem cells derived from a patient with autosomal dominant familial neurohypophyseal diabetes insipidus caused by a variant in the AVP gene

Toustrup, L. B., Zhou, Y., Kvistgaard, H., Gregersen, N., Rittig, S., Aagaard, L., Corydon, T. J., Luo, Y. & Christensen, J. H., Mar 2017, In : Stem Cell Research. 19, p. 37-42 6 p.

Mitochondrial Spare Respiratory Capacity Is Negatively Correlated With Nuclear Reprogramming Efficiency

Zhou, Y., Al-Saaidi, R. A., Guerra, P. F., Freude, K. K., Olsen, R. K. J., Jensen, U. B., Gregersen, N., Hyttel, P., Bolund, L., Aagaard, L., Bross, P. & Luo, Y., Feb 2017, In : Stem Cells and Development. 26, 3, p. 166-176 11 p.

Evaluation of porcine stem cells competence for somatic cell nuclear transfer and production of cloned animals

Secher, J., Liu, Y., Petkov, S., Luo, Y., Li, D., Hall, V. J., Schmidt, M., Callesen, H., Bentzon, J. F., Sørensen, C. B., Freude, K. K. & Hyttel, P., 2017, In : Animal Reproduction Science. 178, p. 40-49

Fusion of SpCas9 to E.coli Rec A protein enhances CRISPR-Cas9 mediated gene knockout in mammalian cells

Lin, L., Petersen, T. S., Jensen, K. T., Bolund, L., Kühn, R. & Luo, Y., 2017, In : Journal of Biotechnology. 247, p. 42-29 8 p.

Patient iPSC-Derived Neurons for Disease Modeling of Frontotemporal Dementia with Mutation in CHMP2B

Zhang, Y., Schmid, B., Nikolaisen, N. K., Rasmussen, M. A., Aldana, B. I., Agger, M., Calloe, K., Stummann, T. C., Larsen, H. M., Nielsen, T. T., Huang, J., Xu, F., Liu, X., Bolund, L., Meyer, M., Bak, L. K., Waagepetersen, H. S., Luo, Y., Nielsen, J. E., Holst, B., Clausen, C., Hyttel, P., Freude, K. K. & FReJA Consortium, 2017, In : Stem Cell Reports. 8, 3

Systematic in vitro and in vivo characterization of Leukemia-inhibiting factor- and Fibroblast growth factor-derived porcine induced pluripotent stem cells

Secher, J. O., Ceylan, A., Mazzoni, G., Mashayekhi, K., Li, T., Muenthaisong, S., Nielsen, T. T., Li, D., Li, S., Petkov, S., Cirera, S., Luo, Y., Thombs, L., Kadarmideen, H. N., Dinnyes, A., Bolund, L., Roelen, B. A. J., Schmidt, M., Callesen, H., Hyttel, P. & Freude, K. K., 2017, In : Molecular Reproduction and Development. 84, 3, p. 229-245 17 p.

The Effects of Voluntary Physical Exercise-Activated Neurotrophic Signaling in Rat Hippocampus on mRNA Levels of Downstream Signaling Molecules

Sølvsten, C., Daugaard, T. F., Luo, Y., de Paoli, F. V., Christensen, J. H. & Nielsen, A. L., 2017, In : Journal of Molecular Neuroscience. 62, 2, p. 142-153

A Golden Gate-based Protocol for Assembly of Multiplexed gRNA Expression Arrays for CRISPR/Cas9

Vad-Nielsen, J., Lin, L., Jensen, K. T., Nielsen, A. L. & Luo, Y., 5 Dec 2016, In : Bio-protocol. 6, 23, e2059.

Generation of induced pluripotent stem cells (iPSCs) stably expressing CRISPR-based synergistic activation mediator (SAM)

Xiong, K., Zhou, Y., Hyttel, P., Bolund, L., Freude, K. K. & Luo, Y., 17 Nov 2016, In : Stem Cell Research. 17, 3, p. 665-669 5 p.

CRISPR-mediated multiplexed genetic manipulation

Lin, L., Vad-Nielsen, J. & Luo, Y., 15 Nov 2016, In : OncoTargets and Therapy.

The CRISPR-Cas9 Minipig

Berthelsen, M. F., Møller, S., Callesen, H., Dagnæs-Hansen, F., Luo, Y., Jakobsen, J. E. & Thomsen, M. K., 22 Sep 2016. 1 p.

Golden Gate Assembly of CRISPR gRNA expression array for simultaneously targeting multiple genes

Vad-Nielsen, J., Lin, L., Bolund, L., Nielsen, A. L. & Luo, Y., 13 May 2016, In : Cellular and Molecular Life Sciences. 73, 22, p. 4315–4325

Pig model for diabetes

Luo, Y. & Bolund, L., 3 Mar 2016, Priority date 28 Aug 2014, Priority No. WO2016029919 A1

Enhanced genome editing in mammalian cells with a modified dual-fluorescent surrogate system

Zhou, Y., Liu, Y., Hussmann, D., Brøgger, P., Al-Saaidi, R. A., Tan, S., Lin, L., Petersen, T. S., Zhou, G. Q., Bross, P., Aagaard, L., Klein, T., Rønn, S. G., Pedersen, H. D., Bolund, L., Nielsen, A. L., Sørensen, C. B. & Luo, Y., 11 Jan 2016, In : Cellular and Molecular Life Sciences. 73, 13, p. 2543-63 21 p.

Genetically modified pig models for neurodegenerative disorders

Holm, I. E., Alstrup, A. K. O. & Luo, Y., Jan 2016, In : Journal of Pathology. 238, 2, p. 267-87 21 p.

Precision Epigenome Editing – Promise and Challenge

Fløe, L. & Luo, Y., 2016, In : Biozoom. 4

Targeted, homology-driven gene insertion in stem cells by ZFN-loaded 'all-in-one' lentiviral vectors

Cai, Y., Laustsen, A., Zhou, Y., Sun, C., Anderson, M. V., Li, S., Uldbjerg, N., Luo, Y., Jakobsen, M. R. & Mikkelsen, J. G., 2016, In : eLife. 5

EWS and FUS bind a subset of transcribed genes encoding proteins enriched in RNA regulatory functions

Luo, Y., Friis, J. B., Fernandes, A. M., Li, S., Fryland, T., Børglum, A., Bolund, L. & Nielsen, A. L., Nov 2015, In : BMC Genomics. 16, 17 p.

The Epigenetic Reprogramming Roadmap in Generation of iPSCs from Somatic Cells

Brix, J., Zhou, Y. & Luo, Y., Oct 2015, In : Journal of Genetics and Genomics.

Towards personalized regenerative cell therapy: Mesenchymal stem cells derived from human induced pluripotent stem cells

Lin, L., Bolund, L. & Luo, Y., 23 Jul 2015, In : Current Stem Cell Research & Therapy.

Mesenchymal stem cells derived from human induced pluripotent stem cells retain adequate osteogenicity and chondrogenicity but less adipogenicity

Kang, R., Zhou, Y., Tan, S., Zhou, G., Aagaard, L., Xie, L., Bünger, C., Bolund, L. & Luo, Y., 2015, In : Stem Cell Research & Therapy. 6, 1, p. 144

Targeted Porcine Genome Engineering with TALENs

Luo, Y., Lin, L., Golas, M. M., Sørensen, C. B. & Bolund, L., 2015, Somatic Genome Manipulation : Advances, Methods and Applications. Springer New York, p. 17-33

Clock-controlled StAR's expression and corticosterone production contribute to the endotoxemia immune response

Wang, J., Luo, Y., Wang, K., Wang, Y., Zhang, X., Teng, H. & Sun, Z., 25 Nov 2014, In : Chronobiology International. p. 1-10 10 p.

Efficient construction of rAAV-based gene targeting vectors by Golden Gate cloning

Luo, Y., Lin, L., Bolund, L. & Sørensen, C. B., May 2014, In : BioTechniques. 56, 5, p. 263-8 6 p.

Effects of high hydrostatic pressure on genomic expression profiling of porcine parthenogenetic activated and cloned embryos

Lin, L., Luo, Y., Sørensen, P., Prætorius, H., Vajta, G., Callesen, H., Pribenszky, C., Bolund, L. & Kristensen, T. N., 2014, In : Reproduction, Fertility and Development. 26, p. 469-484 16 p.

Efficient genome engineering using rAAV and TALENs in pigs

Luo, Y., Viuff, B. M., Lin, L., Li, R., Schmidt, M., Rønn, S. G., Pedersen, H. D., Callesen, H., Sørensen, C. B., Brünner, N., Klein, T. & Bolund, L., 2014, In : Transgenic Research. 23, 1, p. 189-190

Oesteogenesis of Human Induced Pluripotent Stem Cells Derived Mesenchymal Stem Cells on hydroxyapatite contained Nanofibers

Kang, R., Luo, Y., Zou, L., Xie, L., Lysdahl, H., Jiang, X., Chen, C., Bolund, L., Chen, M., Besenbacher, F. & Bunger, C. E., 2014, In : R S C Advances. 4, 11, p. 5734-5739

A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds

Zou, L., Luo, Y., Chen, M., Wang, G., Ding, M., Petersen, C. C., Kang, R., Dagnaes-Hansen, F., Zeng, Y., Lv, N., Ma, Q., Le, D. Q. S., Besenbacher, F., Bolund, L., Jensen, T. G., Kjems, J., Pu, W. T. & Bünger, C., 22 Jul 2013, In : Scientific Reports. 3, 10 p., 2243.

Gene Expression Responses to FUS, EWS, and TAF15 Reduction and Stress Granule Sequestration Analyses Identifies FET-Protein Non-Redundant Functions

Blechingberg, J., Luo, Y., Bolund, L., Damgaard, C. K. & Nielsen, A. L., 25 Sep 2012, In : P L o S One. 7, 9, p. e46251 15 p.

Development of BRCA1 Knockout Pigs and Transcriptome Studies of Disease Progression

Luo, Y., Mar 2012

Genetically modified pigs for biomedical research

Luo, Y., Lin, L., Bolund, L., Jensen, T. G. & Sørensen, C. B., 2012, In : Journal of Inherited Metabolic Disease. 35, 4, p. 695-713

Pig gene knockout by rAAV-mediated homologous recombination: comparison of BRCA1 gene knockout efficiency in Yucatan and Göttingen fibroblasts with slightly different target sequences

Luo, Y., Bolund, L. & Sørensen, C. B., 2012, In : Transgenic Research. 21, p. 671-676

Regulation of Peripheral Clock to Oscillation of Substance P Contributes to Circadian Inflammatory Pain

Zhang, J., Li, H., Teng, H., Zhang, T., Luo, Y., Zhao, M., Li, Y-Q. & Sun, Z. S., 2012, In : Anesthesiology News. 117, 1

Targeted Genome Editing by Recombinant Adeno-Associated Virus (rAAV) Vectors for Generating Genetically Modified Pigs

Luo, Y., Kofod-Olsen, E., Christensen, R., Sørensen, C. B. & Bolund, L., 2012, In : Journal of Genetics and Genomics. 39, 6, p. 269-74 6 p.

Amino acid-induced gene expression profiling in clonal β-cell line INS-1E cells

Liu, Z., Luo, Y., Jeppesen, P. B., Gregersen, S. & Hermansen, K., 1 Feb 2011, In : Diabetes - Metabolism: Research and Reviews (Print Edition). 27, 2, p. 120-76 57 p.

An improved PCR strategy for fast screening of specific and random integrations in rAAV-mediated gene targeted cell clones

Luo, Y., Bolund, L. & Sørensen, C. B., 2011, In : BMC Research Notes. 4, p. 246

Comparison of gene expression and genome-wide DNA methylation profiling between phenotypically normal cloned pigs and conventionally bred controls

Fei, G., Luo, Y., Li, S., Li, J., Lin, L., Nielsen, A. L., Sørensen, C. B., Vajta, G., Wang, J., Zhang, X., Du, Y., Yang, H. & Bolund, L., 2011, In : P L o S One. 6, 10, p. e25901

DNA Methylation in Peripheral Blood Cells of Pigs Cloned by Somatic Cell Nuclear Transfer

Gao, F., Li, S., Lin, L., Li, J., Luo, Y., Zhang, X., Nielsen, A. L. & Bolund, L., 2011, In : Cellular Reprogramming. 13, 4, p. 307-314

High efficiency of BRCA1 knockout using rAAV-mediated gene targeting: developing a pig model for breast cancer

Luo, Y., Li, J., Liu, Y., Lin, L., Du, Y., Li, S., Yang, H., Vajta, G., Callesen, H., Bolund, L. & Sørensen, C. B., 2011, In : Transgenic Research. 20, p. 975-988

Expression Profiling Reveals A Positive Regulation by Mper2 on Circadian Rhythm of Cytotoxicity Receptors: Ly49c and Nkg2d

Luo, Y., Tian, W., Cai, L., Wang, Y., Zhang, J., Teng, H., Du, J. & Sun, Z. S., 2009, In : Chronobiology International. 26, 8, p. 1514-1544

Activities

The Roslin Institute

Yonglun Luo (Visiting researcher)
15 Apr 201315 Jun 2013

ESOF2012

Yonglun Luo (Participant)
11 Jul 201216 Jul 2012

Development of BRCA1 Knockout Pigs and Transcriptome Studies of Disease Progression

Yonglun Luo (Invited speaker)
13 Jan 2012

2nd International Symposium on Breast Cancer Prevention

Yonglun Luo (Invited speaker)
9 Oct 201111 Oct 2011

MEDICINSKGENETIK

Yonglun Luo (Other)
6 Jan 201112 Jan 2011

Transgenesis and Gene Targeting using Recombinant Adeno-Associated Virus (rAAV): External PhD course

Yonglun Luo (Lecturer)
Oct 2010Nov 2010

CSH - Asia Meeting: Human Genetics & Genomics

Yonglun Luo (Participant)
6 Sep 201010 Sep 2010

American Society of Gene Therapy 13th Annual Meeting

Yonglun Luo (Participant)
19 May 201022 May 2010