Vladimir Matchkov

Trophic sympathetic influence weakens pro-contractile role of Cl channels in rat arteries during postnatal maturation

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Trophic sympathetic influence weakens pro-contractile role of Cl channels in rat arteries during postnatal maturation. / Kostyunina, Daria S.; Zhang, Lin; Shvetsova, Anastasia A.; Selivanova, Ekaterina K.; Tarasova, Olga S.; Matchkov, Vladimir V.; Gaynullina, Dina K.

In: Scientific Reports, Vol. 10, No. 1, 20002, 12.2020.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Kostyunina, DS, Zhang, L, Shvetsova, AA, Selivanova, EK, Tarasova, OS, Matchkov, VV & Gaynullina, DK 2020, 'Trophic sympathetic influence weakens pro-contractile role of Cl channels in rat arteries during postnatal maturation', Scientific Reports, vol. 10, no. 1, 20002. https://doi.org/10.1038/s41598-020-77092-0

APA

Kostyunina, D. S., Zhang, L., Shvetsova, A. A., Selivanova, E. K., Tarasova, O. S., Matchkov, V. V., & Gaynullina, D. K. (2020). Trophic sympathetic influence weakens pro-contractile role of Cl channels in rat arteries during postnatal maturation. Scientific Reports, 10(1), [20002]. https://doi.org/10.1038/s41598-020-77092-0

CBE

Kostyunina DS, Zhang L, Shvetsova AA, Selivanova EK, Tarasova OS, Matchkov VV, Gaynullina DK. 2020. Trophic sympathetic influence weakens pro-contractile role of Cl channels in rat arteries during postnatal maturation. Scientific Reports. 10(1):Article 20002. https://doi.org/10.1038/s41598-020-77092-0

MLA

Vancouver

Kostyunina DS, Zhang L, Shvetsova AA, Selivanova EK, Tarasova OS, Matchkov VV et al. Trophic sympathetic influence weakens pro-contractile role of Cl channels in rat arteries during postnatal maturation. Scientific Reports. 2020 Dec;10(1). 20002. https://doi.org/10.1038/s41598-020-77092-0

Author

Kostyunina, Daria S. ; Zhang, Lin ; Shvetsova, Anastasia A. ; Selivanova, Ekaterina K. ; Tarasova, Olga S. ; Matchkov, Vladimir V. ; Gaynullina, Dina K. / Trophic sympathetic influence weakens pro-contractile role of Cl channels in rat arteries during postnatal maturation. In: Scientific Reports. 2020 ; Vol. 10, No. 1.

Bibtex

@article{3b14f91b0d3740829e27cc2ee2f88a3c,
title = "Trophic sympathetic influence weakens pro-contractile role of Cl− channels in rat arteries during postnatal maturation",
abstract = "Membrane transporters and their functional contribution in vasculature change during early postnatal development. Here we tested the hypothesis that the contribution of Cl− channels to arterial contraction declines during early postnatal development and this decline is associated with the trophic sympathetic influence. Endothelium‐denuded saphenous arteries from 1- to 2-week-old and 2- to 3-month-old male rats were used. Arterial contraction was assessed in the isometric myograph, in some experiments combined with measurements of membrane potential. mRNA and protein levels were determined by qPCR and Western blot. Sympathectomy was performed by treatment with guanethidine from the first postnatal day until 8–9-week age. Cl− substitution in the solution as well as Cl−-channel blockers (MONNA, DIDS) had larger suppressive effect on the methoxamine-induced arterial contraction and methoxamine-induced depolarization of smooth muscle cells in 1- to 2-week-old compared to 2- to 3-month-old rats. Vasculature of younger group demonstrated elevated expression levels of TMEM16A and bestrophin 3. Chronic sympathectomy increased Cl− contribution to arterial contraction in 2-month-old rats that was associated with an increased TMEM16A expression level. Our study demonstrates that contribution of Cl− channels to agonist-induced arterial contraction and depolarization decreases during postnatal development. This postnatal decline is associated with sympathetic nerves development.",
author = "Kostyunina, {Daria S.} and Lin Zhang and Shvetsova, {Anastasia A.} and Selivanova, {Ekaterina K.} and Tarasova, {Olga S.} and Matchkov, {Vladimir V.} and Gaynullina, {Dina K.}",
year = "2020",
month = dec,
doi = "10.1038/s41598-020-77092-0",
language = "English",
volume = "10",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - Trophic sympathetic influence weakens pro-contractile role of Cl− channels in rat arteries during postnatal maturation

AU - Kostyunina, Daria S.

AU - Zhang, Lin

AU - Shvetsova, Anastasia A.

AU - Selivanova, Ekaterina K.

AU - Tarasova, Olga S.

AU - Matchkov, Vladimir V.

AU - Gaynullina, Dina K.

PY - 2020/12

Y1 - 2020/12

N2 - Membrane transporters and their functional contribution in vasculature change during early postnatal development. Here we tested the hypothesis that the contribution of Cl− channels to arterial contraction declines during early postnatal development and this decline is associated with the trophic sympathetic influence. Endothelium‐denuded saphenous arteries from 1- to 2-week-old and 2- to 3-month-old male rats were used. Arterial contraction was assessed in the isometric myograph, in some experiments combined with measurements of membrane potential. mRNA and protein levels were determined by qPCR and Western blot. Sympathectomy was performed by treatment with guanethidine from the first postnatal day until 8–9-week age. Cl− substitution in the solution as well as Cl−-channel blockers (MONNA, DIDS) had larger suppressive effect on the methoxamine-induced arterial contraction and methoxamine-induced depolarization of smooth muscle cells in 1- to 2-week-old compared to 2- to 3-month-old rats. Vasculature of younger group demonstrated elevated expression levels of TMEM16A and bestrophin 3. Chronic sympathectomy increased Cl− contribution to arterial contraction in 2-month-old rats that was associated with an increased TMEM16A expression level. Our study demonstrates that contribution of Cl− channels to agonist-induced arterial contraction and depolarization decreases during postnatal development. This postnatal decline is associated with sympathetic nerves development.

AB - Membrane transporters and their functional contribution in vasculature change during early postnatal development. Here we tested the hypothesis that the contribution of Cl− channels to arterial contraction declines during early postnatal development and this decline is associated with the trophic sympathetic influence. Endothelium‐denuded saphenous arteries from 1- to 2-week-old and 2- to 3-month-old male rats were used. Arterial contraction was assessed in the isometric myograph, in some experiments combined with measurements of membrane potential. mRNA and protein levels were determined by qPCR and Western blot. Sympathectomy was performed by treatment with guanethidine from the first postnatal day until 8–9-week age. Cl− substitution in the solution as well as Cl−-channel blockers (MONNA, DIDS) had larger suppressive effect on the methoxamine-induced arterial contraction and methoxamine-induced depolarization of smooth muscle cells in 1- to 2-week-old compared to 2- to 3-month-old rats. Vasculature of younger group demonstrated elevated expression levels of TMEM16A and bestrophin 3. Chronic sympathectomy increased Cl− contribution to arterial contraction in 2-month-old rats that was associated with an increased TMEM16A expression level. Our study demonstrates that contribution of Cl− channels to agonist-induced arterial contraction and depolarization decreases during postnatal development. This postnatal decline is associated with sympathetic nerves development.

UR - http://www.scopus.com/inward/record.url?scp=85096088795&partnerID=8YFLogxK

U2 - 10.1038/s41598-020-77092-0

DO - 10.1038/s41598-020-77092-0

M3 - Journal article

C2 - 33203943

AN - SCOPUS:85096088795

VL - 10

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 20002

ER -