Vladimir Matchkov

Trophic sympathetic influence weakens pro-contractile role of Cl channels in rat arteries during postnatal maturation

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  • Daria S. Kostyunina, Lomonosov Moscow State University, University College Dublin
  • ,
  • Lin Zhang, Beijing Sport University
  • ,
  • Anastasia A. Shvetsova, Lomonosov Moscow State University
  • ,
  • Ekaterina K. Selivanova, Lomonosov Moscow State University
  • ,
  • Olga S. Tarasova, Lomonosov Moscow State University, Russian Academy of Sciences
  • ,
  • Vladimir V. Matchkov
  • Dina K. Gaynullina, Lomonosov Moscow State University

Membrane transporters and their functional contribution in vasculature change during early postnatal development. Here we tested the hypothesis that the contribution of Cl channels to arterial contraction declines during early postnatal development and this decline is associated with the trophic sympathetic influence. Endothelium‐denuded saphenous arteries from 1- to 2-week-old and 2- to 3-month-old male rats were used. Arterial contraction was assessed in the isometric myograph, in some experiments combined with measurements of membrane potential. mRNA and protein levels were determined by qPCR and Western blot. Sympathectomy was performed by treatment with guanethidine from the first postnatal day until 8–9-week age. Cl substitution in the solution as well as Cl-channel blockers (MONNA, DIDS) had larger suppressive effect on the methoxamine-induced arterial contraction and methoxamine-induced depolarization of smooth muscle cells in 1- to 2-week-old compared to 2- to 3-month-old rats. Vasculature of younger group demonstrated elevated expression levels of TMEM16A and bestrophin 3. Chronic sympathectomy increased Cl contribution to arterial contraction in 2-month-old rats that was associated with an increased TMEM16A expression level. Our study demonstrates that contribution of Cl channels to agonist-induced arterial contraction and depolarization decreases during postnatal development. This postnatal decline is associated with sympathetic nerves development.

Original languageEnglish
Article number20002
JournalScientific Reports
Volume10
Issue1
ISSN2045-2322
DOIs
Publication statusPublished - Dec 2020

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