Vladimir Matchkov

The ouabain-sensitive isoform of Na+-pump regulates vascular gap junctions via interaction with the Na+/Ca2+-exchanger in membrane microdomain

Research output: Contribution to conferencePosterResearch

Standard

The ouabain-sensitive isoform of Na+-pump regulates vascular gap junctions via interaction with the Na+/Ca2+-exchanger in membrane microdomain. / Matchkov, Vladimir; Nilsson, Holger; Aalkjær, Christian.

2008. Poster session presented at 13th Annual Meeting of the European Council of Cardiovascular Research , Nice, France.

Research output: Contribution to conferencePosterResearch

Harvard

Matchkov, V, Nilsson, H & Aalkjær, C 2008, 'The ouabain-sensitive isoform of Na+-pump regulates vascular gap junctions via interaction with the Na+/Ca2+-exchanger in membrane microdomain', 13th Annual Meeting of the European Council of Cardiovascular Research , Nice, France, 10/10/2008 - 12/10/2008.

APA

Matchkov, V., Nilsson, H., & Aalkjær, C. (2008). The ouabain-sensitive isoform of Na+-pump regulates vascular gap junctions via interaction with the Na+/Ca2+-exchanger in membrane microdomain. Poster session presented at 13th Annual Meeting of the European Council of Cardiovascular Research , Nice, France.

CBE

Matchkov V, Nilsson H, Aalkjær C. 2008. The ouabain-sensitive isoform of Na+-pump regulates vascular gap junctions via interaction with the Na+/Ca2+-exchanger in membrane microdomain. Poster session presented at 13th Annual Meeting of the European Council of Cardiovascular Research , Nice, France.

MLA

Matchkov, Vladimir, Holger Nilsson, and Christian Aalkjær The ouabain-sensitive isoform of Na+-pump regulates vascular gap junctions via interaction with the Na+/Ca2+-exchanger in membrane microdomain. <strong>13th Annual Meeting of the European Council of Cardiovascular Research </strong>, 10 Oct 2008, Nice, France, Poster, 2008. 1 p.

Vancouver

Matchkov V, Nilsson H, Aalkjær C. The ouabain-sensitive isoform of Na+-pump regulates vascular gap junctions via interaction with the Na+/Ca2+-exchanger in membrane microdomain. 2008. Poster session presented at 13th Annual Meeting of the European Council of Cardiovascular Research , Nice, France.

Author

Matchkov, Vladimir ; Nilsson, Holger ; Aalkjær, Christian. / The ouabain-sensitive isoform of Na+-pump regulates vascular gap junctions via interaction with the Na+/Ca2+-exchanger in membrane microdomain. Poster session presented at 13th Annual Meeting of the European Council of Cardiovascular Research , Nice, France.1 p.

Bibtex

@conference{ffa02820694c11dd9251000ea68e967b,
title = "The ouabain-sensitive isoform of Na+-pump regulates vascular gap junctions via interaction with the Na+/Ca2+-exchanger in membrane microdomain",
abstract = "Ouabain, an inhibitor of the Na+-pump, has been shown to inhibit intercellular communication. We have recently shown that gap junctions between vascular smooth muscle cells (SMCs) are regulated through an interaction between a ouabain-sensitive isoform of the Na+-pump and the Na+/Ca2+-exchanger leading to increases in [Ca2+]i in discrete areas near the plasma membrane. This suggests close association of these transport proteins in microdomains. Using PCR and co-immunoprecipitation we aimed to test this hypothesis in SMCs from mesenteric small arteries and in A7r5 cell line. Intercellular electrical coupling was evaluated in functional studies. SMCs were electrically uncoupled when the ouabain-sensitive Na+-pump was inhibited by 10 mM ouabain. Inhibition of the Na+/Ca2+-exchanger with 1 mM SEA0400 also uncoupled the SMCs. Depletion of [Na+]i and clamping [Ca2+]i at low levels prevented the uncoupling. Ten mM ouabain evoked spatially restricted [Ca2+]i transients along the cell periphery but not in the center of the cell. mRNA for all three isoforms of the Na+-pump α subunit were found in SMCs but only ouabain-sensitive α2 subunit was specifically co-immunoprecipitated with the Na+/Ca2+-exchanger-1 and connexin-43. The α3 Na+-pump subunit was not associated with these proteins but co-immunoprecipitated with caveolin-1. Based on these experiments we suggest that α2 Na+ -pump subunit is involved in regulation of the intercellular communication via interaction with the Na+/Ca2+-exchanger-1 leading to local [Ca2+]i transients near the membrane which block the closely associated connexin-43 containing gap junctions.",
author = "Vladimir Matchkov and Holger Nilsson and Christian Aalkj{\ae}r",
year = "2008",
language = "English",
note = "null ; Conference date: 10-10-2008 Through 12-10-2008",

}

RIS

TY - CONF

T1 - The ouabain-sensitive isoform of Na+-pump regulates vascular gap junctions via interaction with the Na+/Ca2+-exchanger in membrane microdomain

AU - Matchkov, Vladimir

AU - Nilsson, Holger

AU - Aalkjær, Christian

N1 - Conference code: 13

PY - 2008

Y1 - 2008

N2 - Ouabain, an inhibitor of the Na+-pump, has been shown to inhibit intercellular communication. We have recently shown that gap junctions between vascular smooth muscle cells (SMCs) are regulated through an interaction between a ouabain-sensitive isoform of the Na+-pump and the Na+/Ca2+-exchanger leading to increases in [Ca2+]i in discrete areas near the plasma membrane. This suggests close association of these transport proteins in microdomains. Using PCR and co-immunoprecipitation we aimed to test this hypothesis in SMCs from mesenteric small arteries and in A7r5 cell line. Intercellular electrical coupling was evaluated in functional studies. SMCs were electrically uncoupled when the ouabain-sensitive Na+-pump was inhibited by 10 mM ouabain. Inhibition of the Na+/Ca2+-exchanger with 1 mM SEA0400 also uncoupled the SMCs. Depletion of [Na+]i and clamping [Ca2+]i at low levels prevented the uncoupling. Ten mM ouabain evoked spatially restricted [Ca2+]i transients along the cell periphery but not in the center of the cell. mRNA for all three isoforms of the Na+-pump α subunit were found in SMCs but only ouabain-sensitive α2 subunit was specifically co-immunoprecipitated with the Na+/Ca2+-exchanger-1 and connexin-43. The α3 Na+-pump subunit was not associated with these proteins but co-immunoprecipitated with caveolin-1. Based on these experiments we suggest that α2 Na+ -pump subunit is involved in regulation of the intercellular communication via interaction with the Na+/Ca2+-exchanger-1 leading to local [Ca2+]i transients near the membrane which block the closely associated connexin-43 containing gap junctions.

AB - Ouabain, an inhibitor of the Na+-pump, has been shown to inhibit intercellular communication. We have recently shown that gap junctions between vascular smooth muscle cells (SMCs) are regulated through an interaction between a ouabain-sensitive isoform of the Na+-pump and the Na+/Ca2+-exchanger leading to increases in [Ca2+]i in discrete areas near the plasma membrane. This suggests close association of these transport proteins in microdomains. Using PCR and co-immunoprecipitation we aimed to test this hypothesis in SMCs from mesenteric small arteries and in A7r5 cell line. Intercellular electrical coupling was evaluated in functional studies. SMCs were electrically uncoupled when the ouabain-sensitive Na+-pump was inhibited by 10 mM ouabain. Inhibition of the Na+/Ca2+-exchanger with 1 mM SEA0400 also uncoupled the SMCs. Depletion of [Na+]i and clamping [Ca2+]i at low levels prevented the uncoupling. Ten mM ouabain evoked spatially restricted [Ca2+]i transients along the cell periphery but not in the center of the cell. mRNA for all three isoforms of the Na+-pump α subunit were found in SMCs but only ouabain-sensitive α2 subunit was specifically co-immunoprecipitated with the Na+/Ca2+-exchanger-1 and connexin-43. The α3 Na+-pump subunit was not associated with these proteins but co-immunoprecipitated with caveolin-1. Based on these experiments we suggest that α2 Na+ -pump subunit is involved in regulation of the intercellular communication via interaction with the Na+/Ca2+-exchanger-1 leading to local [Ca2+]i transients near the membrane which block the closely associated connexin-43 containing gap junctions.

M3 - Poster

Y2 - 10 October 2008 through 12 October 2008

ER -