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Department of Biomedicine

Vladimir Matchkov

Perivascular Adipose Tissue Contributes to the Modulation of Vascular Tone in vivo

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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Perivascular Adipose Tissue Contributes to the Modulation of Vascular Tone in vivo. / Saxton, Sophie N; Withers, Sarah B; Nyvad, Jakob et al.

In: Journal of Vascular Research, Vol. 56, No. 6, 2019, p. 320-332.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Vancouver

Saxton SN, Withers SB, Nyvad J, Mazur A, Matchkov V, Heagerty AM et al. Perivascular Adipose Tissue Contributes to the Modulation of Vascular Tone in vivo. Journal of Vascular Research. 2019;56(6):320-332. Epub 2019 Sep 24. doi: 10.1159/000502689

Author

Saxton, Sophie N ; Withers, Sarah B ; Nyvad, Jakob et al. / Perivascular Adipose Tissue Contributes to the Modulation of Vascular Tone in vivo. In: Journal of Vascular Research. 2019 ; Vol. 56, No. 6. pp. 320-332.

Bibtex

@article{9589ec45e10642d5ad1afc3332dc031f,

title = "Perivascular Adipose Tissue Contributes to the Modulation of Vascular Tone in vivo",

abstract = "BACKGROUND: Perivascular adipose tissue (PVAT) reduces vascular tone in isolated arteries in vitro, however there are no studies of PVAT effects on vascular tone in vivo. In vitro adipocyte β3-adrenoceptors play a role in PVAT function via secretion of the vasodilator adiponectin.OBJECTIVE: We have investigated the effects of PVAT on vessel diameter in vivo, and the contributions of β3-adrenoceptors and adiponectin.METHOD: In anaesthetised rats, sections of the intact mesenteric bed were visualised and the diameter of arteries was recorded. Arteries were stimulated with electrical field stimulation (EFS), noradrenaline (NA), arginine-vasopressin (AVP), and acetylcholine (Ach).RESULTS: We report that in vivo, stimulation of PVAT with EFS, NA, and AVP evokes a local anti-constrictive effect on the artery, whilst PVAT exerts a pro-contractile effect on arteries subjected to Ach. The anti-constrictive effect of PVAT stimulated with EFS and NA was significantly reduced using β3-adrenoceptor inhibition, and activation of β3-adrenoceptors potentiated the anti-constrictive effect of vessels stimulated with EFS, NA, and AVP. The β3-adrenoceptor agonist had no effect on mesenteric arteries with PVAT removed. A blocking peptide for adiponectin receptor 1 polyclonal antibody reduced the PVAT anti-constrictive effect in arteries stimulated with EFS and NA, indicating that adiponectin may be the anti-constrictive factor released upon β3-adrenoceptor activation.CONCLUSIONS: These results clearly demonstrate that PVAT plays a paracrine role in regulating local vascular tone in vivo, and therefore may contribute to the modulation of blood pressure. This effect is mediated via adipocyte β3-adrenoceptors, which may trigger release of the vasodilator adiponectin.",

keywords = "Adipocytes, Adrenoceptors, Vascular tone",

author = "Saxton, {Sophie N} and Withers, {Sarah B} and Jakob Nyvad and Aleksandra Mazur and Vladimir Matchkov and Heagerty, {Anthony M} and Christian Aalkj{\ae}r",

note = "{\textcopyright} 2019 S. Karger AG, Basel.",

year = "2019",

doi = "10.1159/000502689",

language = "English",

volume = "56",

pages = "320--332",

journal = "Journal of Vascular Research",

issn = "1018-1172",

publisher = "S. Karger AG",

number = "6",

}

RIS

TY - JOUR

T1 - Perivascular Adipose Tissue Contributes to the Modulation of Vascular Tone in vivo

AU - Saxton, Sophie N

AU - Withers, Sarah B

AU - Nyvad, Jakob

AU - Mazur, Aleksandra

AU - Matchkov, Vladimir

AU - Heagerty, Anthony M

AU - Aalkjær, Christian

PY - 2019

Y1 - 2019

N2 - BACKGROUND: Perivascular adipose tissue (PVAT) reduces vascular tone in isolated arteries in vitro, however there are no studies of PVAT effects on vascular tone in vivo. In vitro adipocyte β3-adrenoceptors play a role in PVAT function via secretion of the vasodilator adiponectin.OBJECTIVE: We have investigated the effects of PVAT on vessel diameter in vivo, and the contributions of β3-adrenoceptors and adiponectin.METHOD: In anaesthetised rats, sections of the intact mesenteric bed were visualised and the diameter of arteries was recorded. Arteries were stimulated with electrical field stimulation (EFS), noradrenaline (NA), arginine-vasopressin (AVP), and acetylcholine (Ach).RESULTS: We report that in vivo, stimulation of PVAT with EFS, NA, and AVP evokes a local anti-constrictive effect on the artery, whilst PVAT exerts a pro-contractile effect on arteries subjected to Ach. The anti-constrictive effect of PVAT stimulated with EFS and NA was significantly reduced using β3-adrenoceptor inhibition, and activation of β3-adrenoceptors potentiated the anti-constrictive effect of vessels stimulated with EFS, NA, and AVP. The β3-adrenoceptor agonist had no effect on mesenteric arteries with PVAT removed. A blocking peptide for adiponectin receptor 1 polyclonal antibody reduced the PVAT anti-constrictive effect in arteries stimulated with EFS and NA, indicating that adiponectin may be the anti-constrictive factor released upon β3-adrenoceptor activation.CONCLUSIONS: These results clearly demonstrate that PVAT plays a paracrine role in regulating local vascular tone in vivo, and therefore may contribute to the modulation of blood pressure. This effect is mediated via adipocyte β3-adrenoceptors, which may trigger release of the vasodilator adiponectin.

AB - BACKGROUND: Perivascular adipose tissue (PVAT) reduces vascular tone in isolated arteries in vitro, however there are no studies of PVAT effects on vascular tone in vivo. In vitro adipocyte β3-adrenoceptors play a role in PVAT function via secretion of the vasodilator adiponectin.OBJECTIVE: We have investigated the effects of PVAT on vessel diameter in vivo, and the contributions of β3-adrenoceptors and adiponectin.METHOD: In anaesthetised rats, sections of the intact mesenteric bed were visualised and the diameter of arteries was recorded. Arteries were stimulated with electrical field stimulation (EFS), noradrenaline (NA), arginine-vasopressin (AVP), and acetylcholine (Ach).RESULTS: We report that in vivo, stimulation of PVAT with EFS, NA, and AVP evokes a local anti-constrictive effect on the artery, whilst PVAT exerts a pro-contractile effect on arteries subjected to Ach. The anti-constrictive effect of PVAT stimulated with EFS and NA was significantly reduced using β3-adrenoceptor inhibition, and activation of β3-adrenoceptors potentiated the anti-constrictive effect of vessels stimulated with EFS, NA, and AVP. The β3-adrenoceptor agonist had no effect on mesenteric arteries with PVAT removed. A blocking peptide for adiponectin receptor 1 polyclonal antibody reduced the PVAT anti-constrictive effect in arteries stimulated with EFS and NA, indicating that adiponectin may be the anti-constrictive factor released upon β3-adrenoceptor activation.CONCLUSIONS: These results clearly demonstrate that PVAT plays a paracrine role in regulating local vascular tone in vivo, and therefore may contribute to the modulation of blood pressure. This effect is mediated via adipocyte β3-adrenoceptors, which may trigger release of the vasodilator adiponectin.

KW - Adipocytes

KW - Adrenoceptors

KW - Vascular tone

U2 - 10.1159/000502689

DO - 10.1159/000502689

M3 - Journal article

C2 - 31550717

VL - 56

SP - 320

EP - 332

JO - Journal of Vascular Research

JF - Journal of Vascular Research

SN - 1018-1172

IS - 6

ER -