Research output: Contribution to book/anthology/report/proceeding › Article in proceedings › Research › peer-review
Bestrophin-4 was downregulated with small interference (siRNA) in A7r5 cells and in rat mesenteric small arteries in vivo. siRNAs targeting different exons of bestrophin-4 were used. Non-related siRNA and bestrophin-4 siRNA with 2 changed nucleotides were used as the controls for non-specific siRNA action.
Downregulation induced by siRNA was evident by reduced expression of bestrophin-4 mRNA and protein (by 82±6 % and by 54±4 % (n=3 and 6), respectively, for cell culture; in vivo transfection showed similar results). Transfection with siRNAs did not affect other bestrophin isoforms. SMCs (both cultured and in vivo) downregulated for bestrophin-4 demonstrated a significant reduction of ICl,cGMP, while the "classical" Ca2+-activated Cl- current was unaffected. Control transfections had no effect. Surprisingly, arteries with reduced bestrophin-4 were more sensitive to noradrenaline than control vessels. The downregulation of bestrophin-4 significantly reduced the amplitude of arterial vasomotion (by 75±10 % n=6) without significant effect on frequency.
We provide evidence that at least one bestrophin family member - bestrophin-4 - is essential for ICl,cGMP and for generation of vasomotion in rat mesenteric small arteries.
Original language | English |
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Title of host publication | Journal of Vascular Research |
Number of pages | 1 |
Publication year | 2008 |
Pages | 61 |
Publication status | Published - 2008 |
Event | 9TH INTERNATIONAL SYMPOSIUM ON RESISTANCE ARTERIES (ISRA) - HAMILTON ISLAND, Australia Duration: 17 Feb 2008 → 21 Feb 2008 |
Conference | 9TH INTERNATIONAL SYMPOSIUM ON RESISTANCE ARTERIES (ISRA) |
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Land | Australia |
By | HAMILTON ISLAND |
Periode | 17/02/2008 → 21/02/2008 |
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ID: 10325498