Vladimir Matchkov

Distribution of cGMP-dependent and cGMP-independent Ca2+- activated Cl- conductances in smooth muscle cells from different vascular beds and colon

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In the present patch-clamp study we have, for the first time, shown the tissue distribution of a recently characterized cGMP-dependent Ca 2+-activated Cl- conductance [18] in smooth muscle cells freshly isolated from different regions: aorta, pulmonary artery, tail artery, femoral artery, femoral vein, middle cerebral artery, renal artery, portal vein, superior mesenteric artery, mesenteric small artery and colon. The cGMP-dependent Cl- conductance has properties distinct from those of the 'classical' Ca2+-activated Cl- conductances; their different sensitivities to niflumic acid and zinc were here utilized to distinguish them. They were found to be co-expressed in different patterns in smooth muscle cells of different origins. The cGMP-dependent conductance was greater in myocytes from cerebral artery and femoral vein and was greater in the renal artery, aorta, mesenteric small artery, femoral artery and the superior mesenteric artery. The presence of the cGMP-dependent Ca2+-activated Cl- current in smooth muscle cells isolated from the colon demonstrates that this conductance is not limited to the vasculature. The 'classical' Ca2+-activated Cl- conductance was strongly expressed in smooth muscle cells from the portal vein and the tail artery, and noticeably higher in the pulmonary artery.

Original languageEnglish
JournalPflugers Archiv European Journal of Physiology
Volume451
Issue2
Pages (from-to)371-379
Number of pages9
ISSN0031-6768
DOIs
Publication statusPublished - 1 Nov 2005

    Research areas

  • Blood vessel, cGMP, Chloride channel, Colon, Intracellular calcium, Niflumic acid, Smooth muscle, Zinc

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