Vladimir Matchkov

Chronic Ouabain Prevents Radiation-Induced Reduction in the α2 Na,K-ATPase Function in the Rat Diaphragm Muscle

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  • Violetta V. Kravtsova, St. Petersburg State University
  • ,
  • Arina A. Fedorova, St. Petersburg State University
  • ,
  • Maria V. Tishkova, St. Petersburg State University
  • ,
  • Alexandra A. Livanova, St. Petersburg State University
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  • Oleg V. Vetrovoy, RAS - Pavlov Institute of Physiology, St. Petersburg State University
  • ,
  • Alexander G. Markov, St. Petersburg State University
  • ,
  • Vladimir V. Matchkov
  • Igor I. Krivoi, St. Petersburg State University

The damaging effect of ionizing radiation (IR) on skeletal muscle Na,K-ATPase is an open field of research. Considering a therapeutic potential of ouabain, a specific ligand of the Na,K-ATPase, we tested its ability to protect against the IR-induced disturbances of Na,K-ATPase function in rat diaphragm muscle that co-expresses the α1 and α2 isozymes of this protein. Male Wistar rats (n = 26) were subjected to 6-day injections of vehicle (0.9% NaCl) or ouabain (1 µg/kg/day). On the fourth day of injections, rats were exposed to one-time total-body X-ray irradiation (10 Gy), or a sham irradiation. The isolated muscles were studied 72 h post-irradiation. IR decreased the electrogenic contribution of the α2 Na,K-ATPase without affecting its protein content, thereby causing sarcolemma depolarization. IR increased serum concentrations of ouabain, IL-6, and corticosterone, decreased lipid peroxidation, and changed cellular redox status. Chronic ouabain administration prevented IR-induced depolarization and loss of the α2 Na,K-ATPase electrogenic contribution without changing its protein content. This was accompanied with an elevation of ouabain concentration in circulation and with the lack of IR-induced suppression of lipid peroxidation. Given the crucial role of Na,K-ATPase in skeletal muscle performance, these findings may have therapeutic implications as countermeasures for IR-induced muscle pathology.

Original languageEnglish
Article number10921
JournalInternational Journal of Molecular Sciences
Number of pages19
Publication statusPublished - Sept 2022

    Research areas

  • ionizing radiation, Na,K-ATPase isozymes, ouabain, resting membrane potential, skeletal muscle

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