Tomonori Takeuchi

Flp recombinase transgenic mice of C57BL/6 strain for conditional gene targeting

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Flp recombinase transgenic mice of C57BL/6 strain for conditional gene targeting. / Takeuchi, Tomonori; Nomura, Takanori; Tsujita, Mika; Suzuki, Misao; Fuse, Toshimitsu; Mori, Hisashi; Mishina, Masayoshi.

In: Biochemical and Biophysical Research Communications, Vol. 293, No. 3, 10.05.2002, p. 953-7.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Takeuchi, T, Nomura, T, Tsujita, M, Suzuki, M, Fuse, T, Mori, H & Mishina, M 2002, 'Flp recombinase transgenic mice of C57BL/6 strain for conditional gene targeting', Biochemical and Biophysical Research Communications, vol. 293, no. 3, pp. 953-7. https://doi.org/10.1016/S0006-291X(02)00321-2

APA

Takeuchi, T., Nomura, T., Tsujita, M., Suzuki, M., Fuse, T., Mori, H., & Mishina, M. (2002). Flp recombinase transgenic mice of C57BL/6 strain for conditional gene targeting. Biochemical and Biophysical Research Communications, 293(3), 953-7. https://doi.org/10.1016/S0006-291X(02)00321-2

CBE

Takeuchi T, Nomura T, Tsujita M, Suzuki M, Fuse T, Mori H, Mishina M. 2002. Flp recombinase transgenic mice of C57BL/6 strain for conditional gene targeting. Biochemical and Biophysical Research Communications. 293(3):953-7. https://doi.org/10.1016/S0006-291X(02)00321-2

MLA

Vancouver

Takeuchi T, Nomura T, Tsujita M, Suzuki M, Fuse T, Mori H et al. Flp recombinase transgenic mice of C57BL/6 strain for conditional gene targeting. Biochemical and Biophysical Research Communications. 2002 May 10;293(3):953-7. https://doi.org/10.1016/S0006-291X(02)00321-2

Author

Takeuchi, Tomonori ; Nomura, Takanori ; Tsujita, Mika ; Suzuki, Misao ; Fuse, Toshimitsu ; Mori, Hisashi ; Mishina, Masayoshi. / Flp recombinase transgenic mice of C57BL/6 strain for conditional gene targeting. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 293, No. 3. pp. 953-7.

Bibtex

@article{f0f2f5fb1c4c4f21a08fc0a468e1b07b,
title = "Flp recombinase transgenic mice of C57BL/6 strain for conditional gene targeting",
abstract = "We constructed an expression vector of Flp recombinase modified by adding a nuclear localization signal. Injection of the expression vector into fertilized eggs of the C57BL/6 strain yielded transgenic mouse lines expressing the Flp recombinase transgene in the testis. We crossed the transgenic mice to reporter mice carrying the neomycin phosphotransferase gene flanked by target sites of Flp recombinase. Examination of the deletion of the neomycin phosphotransferase gene in the progeny showed that Flp-mediated recombination took place efficiently in vivo in FLP66 transgenic mouse line. These results suggest that the Flp recombinase system is effective in mice and in combination with the Cre recombinase system extends the potentials of gene manipulation in mice. One of the useful applications of FLP66 transgenic mouse line is the removal of marker genes from mice manipulated for the conditional gene targeting with the Cre/loxP system in the pure C57BL/6 genetic background.",
keywords = "Animals, DNA Nucleotidyltransferases/biosynthesis, Gene Targeting/methods, Integrases/genetics, Kanamycin Kinase/genetics, Mice, Mice, Inbred C57BL, Mice, Transgenic, Nuclear Localization Signals, RNA, Messenger/analysis, Recombination, Genetic, Viral Proteins/genetics, beta-Galactosidase/analysis",
author = "Tomonori Takeuchi and Takanori Nomura and Mika Tsujita and Misao Suzuki and Toshimitsu Fuse and Hisashi Mori and Masayoshi Mishina",
note = "(c) 2002 Elsevier Science (USA).",
year = "2002",
month = may,
day = "10",
doi = "10.1016/S0006-291X(02)00321-2",
language = "English",
volume = "293",
pages = "953--7",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier Inc.",
number = "3",

}

RIS

TY - JOUR

T1 - Flp recombinase transgenic mice of C57BL/6 strain for conditional gene targeting

AU - Takeuchi, Tomonori

AU - Nomura, Takanori

AU - Tsujita, Mika

AU - Suzuki, Misao

AU - Fuse, Toshimitsu

AU - Mori, Hisashi

AU - Mishina, Masayoshi

N1 - (c) 2002 Elsevier Science (USA).

PY - 2002/5/10

Y1 - 2002/5/10

N2 - We constructed an expression vector of Flp recombinase modified by adding a nuclear localization signal. Injection of the expression vector into fertilized eggs of the C57BL/6 strain yielded transgenic mouse lines expressing the Flp recombinase transgene in the testis. We crossed the transgenic mice to reporter mice carrying the neomycin phosphotransferase gene flanked by target sites of Flp recombinase. Examination of the deletion of the neomycin phosphotransferase gene in the progeny showed that Flp-mediated recombination took place efficiently in vivo in FLP66 transgenic mouse line. These results suggest that the Flp recombinase system is effective in mice and in combination with the Cre recombinase system extends the potentials of gene manipulation in mice. One of the useful applications of FLP66 transgenic mouse line is the removal of marker genes from mice manipulated for the conditional gene targeting with the Cre/loxP system in the pure C57BL/6 genetic background.

AB - We constructed an expression vector of Flp recombinase modified by adding a nuclear localization signal. Injection of the expression vector into fertilized eggs of the C57BL/6 strain yielded transgenic mouse lines expressing the Flp recombinase transgene in the testis. We crossed the transgenic mice to reporter mice carrying the neomycin phosphotransferase gene flanked by target sites of Flp recombinase. Examination of the deletion of the neomycin phosphotransferase gene in the progeny showed that Flp-mediated recombination took place efficiently in vivo in FLP66 transgenic mouse line. These results suggest that the Flp recombinase system is effective in mice and in combination with the Cre recombinase system extends the potentials of gene manipulation in mice. One of the useful applications of FLP66 transgenic mouse line is the removal of marker genes from mice manipulated for the conditional gene targeting with the Cre/loxP system in the pure C57BL/6 genetic background.

KW - Animals

KW - DNA Nucleotidyltransferases/biosynthesis

KW - Gene Targeting/methods

KW - Integrases/genetics

KW - Kanamycin Kinase/genetics

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Transgenic

KW - Nuclear Localization Signals

KW - RNA, Messenger/analysis

KW - Recombination, Genetic

KW - Viral Proteins/genetics

KW - beta-Galactosidase/analysis

U2 - 10.1016/S0006-291X(02)00321-2

DO - 10.1016/S0006-291X(02)00321-2

M3 - Journal article

C2 - 12051751

VL - 293

SP - 953

EP - 957

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -