Tomonori Takeuchi

Flp recombinase transgenic mice of C57BL/6 strain for conditional gene targeting

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Tomonori Takeuchi
  • Takanori Nomura
  • ,
  • Mika Tsujita
  • ,
  • Misao Suzuki
  • ,
  • Toshimitsu Fuse
  • ,
  • Hisashi Mori
  • ,
  • Masayoshi Mishina

We constructed an expression vector of Flp recombinase modified by adding a nuclear localization signal. Injection of the expression vector into fertilized eggs of the C57BL/6 strain yielded transgenic mouse lines expressing the Flp recombinase transgene in the testis. We crossed the transgenic mice to reporter mice carrying the neomycin phosphotransferase gene flanked by target sites of Flp recombinase. Examination of the deletion of the neomycin phosphotransferase gene in the progeny showed that Flp-mediated recombination took place efficiently in vivo in FLP66 transgenic mouse line. These results suggest that the Flp recombinase system is effective in mice and in combination with the Cre recombinase system extends the potentials of gene manipulation in mice. One of the useful applications of FLP66 transgenic mouse line is the removal of marker genes from mice manipulated for the conditional gene targeting with the Cre/loxP system in the pure C57BL/6 genetic background.

Original languageEnglish
JournalBiochemical and Biophysical Research Communications
Volume293
Issue3
Pages (from-to)953-7
Number of pages5
ISSN0006-291X
DOIs
Publication statusPublished - 10 May 2002
Externally publishedYes

    Research areas

  • Animals, DNA Nucleotidyltransferases/biosynthesis, Gene Targeting/methods, Integrases/genetics, Kanamycin Kinase/genetics, Mice, Mice, Inbred C57BL, Mice, Transgenic, Nuclear Localization Signals, RNA, Messenger/analysis, Recombination, Genetic, Viral Proteins/genetics, beta-Galactosidase/analysis

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