Tine Thach

Recovery and virulence phenotyping of the historic 'Stubbs Collection' of the yellow rust fungus Puccinia striiformis from wheat

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A unique collection of spore samples of Puccinia striiformis, often referred to as the ‘Stubbs collection’, has been stored in liquid nitrogen from 18 to 45 years. A subset of samples representing 35 countries and 28 years was investigated to assess recovery rate, race identity and previously undetected virulence. A new method for recovery using an airbrush sprayer and Novec™ 7100 for inoculating the host plants was highly successful. Ninety-six percent of 231 isolates were recovered. Virulence phenotyping was done using differential sets of wheat genotypes representing specific-resistance genes. A total of 181 samples represented single genotypes (isolates), whereas 40 samples consisted of at least two genotypes. Race identity was confirmed for 102 of 181 single-genotype isolates. The virulence phenotype was updated for additional 44 isolates based on improved resolution of results because of updated and more informative wheat-differential sets. The remaining 35 isolates showed discrepancies for one or more virulences when compared with past results. Additional virulences corresponding to Yr17, Yr25 and Yr27, respectively, which were not assayed originally, were discovered. The value of biological collections for research and plant breeding is discussed along with the challenges of maintaining collections of biotrophic microorganisms.
Original languageEnglish
JournalAnnals of Applied Biology
Pages (from-to)314-326
Number of pages13
Publication statusPublished - Nov 2015

    Research areas

  • wheat yellow rust, genetic resource, virulence, preservation, liquid nitrogen, long-term storage

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