Thomas Damgaard Sandahl

Muscle metabolism and whole blood amino acid profile in patients with liver disease

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Muscle metabolism and whole blood amino acid profile in patients with liver disease. / Dam, Gitte; Sørensen, Michael; Buhl, Mads; Sandahl, Thomas D; Møller, Niels; Ott, Peter; Vilstrup, Hendrik.

In: Scandinavian Journal of Clinical & Laboratory Investigation, Vol. 75, No. 8, 11.2015, p. 674-680.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Dam, G, Sørensen, M, Buhl, M, Sandahl, TD, Møller, N, Ott, P & Vilstrup, H 2015, 'Muscle metabolism and whole blood amino acid profile in patients with liver disease', Scandinavian Journal of Clinical & Laboratory Investigation, vol. 75, no. 8, pp. 674-680.

APA

Dam, G., Sørensen, M., Buhl, M., Sandahl, T. D., Møller, N., Ott, P., & Vilstrup, H. (2015). Muscle metabolism and whole blood amino acid profile in patients with liver disease. Scandinavian Journal of Clinical & Laboratory Investigation, 75(8), 674-680.

CBE

Dam G, Sørensen M, Buhl M, Sandahl TD, Møller N, Ott P, Vilstrup H. 2015. Muscle metabolism and whole blood amino acid profile in patients with liver disease. Scandinavian Journal of Clinical & Laboratory Investigation. 75(8):674-680.

MLA

Dam, Gitte et al. "Muscle metabolism and whole blood amino acid profile in patients with liver disease". Scandinavian Journal of Clinical & Laboratory Investigation. 2015, 75(8). 674-680.

Vancouver

Dam G, Sørensen M, Buhl M, Sandahl TD, Møller N, Ott P et al. Muscle metabolism and whole blood amino acid profile in patients with liver disease. Scandinavian Journal of Clinical & Laboratory Investigation. 2015 Nov;75(8):674-680.

Author

Dam, Gitte ; Sørensen, Michael ; Buhl, Mads ; Sandahl, Thomas D ; Møller, Niels ; Ott, Peter ; Vilstrup, Hendrik. / Muscle metabolism and whole blood amino acid profile in patients with liver disease. In: Scandinavian Journal of Clinical & Laboratory Investigation. 2015 ; Vol. 75, No. 8. pp. 674-680.

Bibtex

@article{3fee93016ad245bca2c5731dcdf2ac95,
title = "Muscle metabolism and whole blood amino acid profile in patients with liver disease",
abstract = "OBJECTIVE: Branched-chain amino acids (BCAA) are used in liver cirrhosis to promote protein synthesis, support ammonia detoxification, and treat hepatic encephalopathy. Cirrhosis leads to subnormal BCAA plasma concentrations and studies indicate that levels are decreased due to their role in muscle ammonia removal. Muscle contribution has not been fully elucidated. We studied muscle amino acid metabolism in six healthy subjects, 13 cirrhosis patients and six patients with an episode of alcoholic hepatitis.METHODS: Subjects had catheters inserted into the femoral artery and vein to obtain arterial (A) and venous (V) concentrations of amino acids (μmol/L blood).RESULTS: BCAA concentrations were lower in patients with cirrhosis compared to healthy subjects (p < 0.05) with no difference between patients with alcoholic hepatitis and the other groups. Muscle BCAA uptake was variable and on average higher in patients with alcoholic hepatitis and patients with stable cirrhosis compared to healthy subjects (mean A-V difference 0.5 and 32 vs. - 12 μmol/L blood) (p = 0.22). The release of aromatic amino acids (AAA) was comparable in the three groups (P > 0.30). The BCAA/AAA (Fischer's ratio) was lower in patients with cirrhosis and patients with alcoholic hepatitis compared to healthy subjects (mean 1.65, 1.17 and 2.73, both p < 0.05) and it was negatively correlated to the Child-Pugh score (p < 0.05).CONCLUSIONS: Patients with liver disease have lower BCAA and higher AAA blood concentrations compared to healthy subjects. The trend towards an increased muscle uptake of BCAA may have contributed but this was not significant.",
author = "Gitte Dam and Michael S{\o}rensen and Mads Buhl and Sandahl, {Thomas D} and Niels M{\o}ller and Peter Ott and Hendrik Vilstrup",
year = "2015",
month = "11",
language = "English",
volume = "75",
pages = "674--680",
journal = "Scandinavian Journal of Clinical & Laboratory Investigation",
issn = "0036-5513",
publisher = "Taylor & francis",
number = "8",

}

RIS

TY - JOUR

T1 - Muscle metabolism and whole blood amino acid profile in patients with liver disease

AU - Dam, Gitte

AU - Sørensen, Michael

AU - Buhl, Mads

AU - Sandahl, Thomas D

AU - Møller, Niels

AU - Ott, Peter

AU - Vilstrup, Hendrik

PY - 2015/11

Y1 - 2015/11

N2 - OBJECTIVE: Branched-chain amino acids (BCAA) are used in liver cirrhosis to promote protein synthesis, support ammonia detoxification, and treat hepatic encephalopathy. Cirrhosis leads to subnormal BCAA plasma concentrations and studies indicate that levels are decreased due to their role in muscle ammonia removal. Muscle contribution has not been fully elucidated. We studied muscle amino acid metabolism in six healthy subjects, 13 cirrhosis patients and six patients with an episode of alcoholic hepatitis.METHODS: Subjects had catheters inserted into the femoral artery and vein to obtain arterial (A) and venous (V) concentrations of amino acids (μmol/L blood).RESULTS: BCAA concentrations were lower in patients with cirrhosis compared to healthy subjects (p < 0.05) with no difference between patients with alcoholic hepatitis and the other groups. Muscle BCAA uptake was variable and on average higher in patients with alcoholic hepatitis and patients with stable cirrhosis compared to healthy subjects (mean A-V difference 0.5 and 32 vs. - 12 μmol/L blood) (p = 0.22). The release of aromatic amino acids (AAA) was comparable in the three groups (P > 0.30). The BCAA/AAA (Fischer's ratio) was lower in patients with cirrhosis and patients with alcoholic hepatitis compared to healthy subjects (mean 1.65, 1.17 and 2.73, both p < 0.05) and it was negatively correlated to the Child-Pugh score (p < 0.05).CONCLUSIONS: Patients with liver disease have lower BCAA and higher AAA blood concentrations compared to healthy subjects. The trend towards an increased muscle uptake of BCAA may have contributed but this was not significant.

AB - OBJECTIVE: Branched-chain amino acids (BCAA) are used in liver cirrhosis to promote protein synthesis, support ammonia detoxification, and treat hepatic encephalopathy. Cirrhosis leads to subnormal BCAA plasma concentrations and studies indicate that levels are decreased due to their role in muscle ammonia removal. Muscle contribution has not been fully elucidated. We studied muscle amino acid metabolism in six healthy subjects, 13 cirrhosis patients and six patients with an episode of alcoholic hepatitis.METHODS: Subjects had catheters inserted into the femoral artery and vein to obtain arterial (A) and venous (V) concentrations of amino acids (μmol/L blood).RESULTS: BCAA concentrations were lower in patients with cirrhosis compared to healthy subjects (p < 0.05) with no difference between patients with alcoholic hepatitis and the other groups. Muscle BCAA uptake was variable and on average higher in patients with alcoholic hepatitis and patients with stable cirrhosis compared to healthy subjects (mean A-V difference 0.5 and 32 vs. - 12 μmol/L blood) (p = 0.22). The release of aromatic amino acids (AAA) was comparable in the three groups (P > 0.30). The BCAA/AAA (Fischer's ratio) was lower in patients with cirrhosis and patients with alcoholic hepatitis compared to healthy subjects (mean 1.65, 1.17 and 2.73, both p < 0.05) and it was negatively correlated to the Child-Pugh score (p < 0.05).CONCLUSIONS: Patients with liver disease have lower BCAA and higher AAA blood concentrations compared to healthy subjects. The trend towards an increased muscle uptake of BCAA may have contributed but this was not significant.

M3 - Journal article

C2 - 26243157

VL - 75

SP - 674

EP - 680

JO - Scandinavian Journal of Clinical & Laboratory Investigation

JF - Scandinavian Journal of Clinical & Laboratory Investigation

SN - 0036-5513

IS - 8

ER -