Thomas Damgaard Sandahl

Mass spectrometry characterization of circulating human serum albumin microheterogeneity in patients with alcoholic hepatitis

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Standard

Mass spectrometry characterization of circulating human serum albumin microheterogeneity in patients with alcoholic hepatitis. / Naldi, Marina; Baldassarre, Maurizio; Domenicali, Marco et al.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 122, 15.04.2016, p. 141-7.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Naldi, M, Baldassarre, M, Domenicali, M, Giannone, FA, Bossi, M, Montomoli, J, Sandahl, TD, Glavind, E, Vilstrup, H, Caraceni, P & Bertucci, C 2016, 'Mass spectrometry characterization of circulating human serum albumin microheterogeneity in patients with alcoholic hepatitis', Journal of Pharmaceutical and Biomedical Analysis, vol. 122, pp. 141-7. https://doi.org/10.1016/j.jpba.2016.01.048

APA

Naldi, M., Baldassarre, M., Domenicali, M., Giannone, F. A., Bossi, M., Montomoli, J., Sandahl, T. D., Glavind, E., Vilstrup, H., Caraceni, P., & Bertucci, C. (2016). Mass spectrometry characterization of circulating human serum albumin microheterogeneity in patients with alcoholic hepatitis. Journal of Pharmaceutical and Biomedical Analysis, 122, 141-7. https://doi.org/10.1016/j.jpba.2016.01.048

CBE

Naldi M, Baldassarre M, Domenicali M, Giannone FA, Bossi M, Montomoli J, Sandahl TD, Glavind E, Vilstrup H, Caraceni P, et al. 2016. Mass spectrometry characterization of circulating human serum albumin microheterogeneity in patients with alcoholic hepatitis. Journal of Pharmaceutical and Biomedical Analysis. 122:141-7. https://doi.org/10.1016/j.jpba.2016.01.048

MLA

Vancouver

Naldi M, Baldassarre M, Domenicali M, Giannone FA, Bossi M, Montomoli J et al. Mass spectrometry characterization of circulating human serum albumin microheterogeneity in patients with alcoholic hepatitis. Journal of Pharmaceutical and Biomedical Analysis. 2016 Apr 15;122:141-7. doi: 10.1016/j.jpba.2016.01.048

Author

Naldi, Marina ; Baldassarre, Maurizio ; Domenicali, Marco et al. / Mass spectrometry characterization of circulating human serum albumin microheterogeneity in patients with alcoholic hepatitis. In: Journal of Pharmaceutical and Biomedical Analysis. 2016 ; Vol. 122. pp. 141-7.

Bibtex

@article{687d7dc283a64420b7febe25cf86babb,
title = "Mass spectrometry characterization of circulating human serum albumin microheterogeneity in patients with alcoholic hepatitis",
abstract = "Human serum albumin (HSA) is the most abundant plasma protein, endowed with several biological properties unrelated to its oncotic power, such as antioxidant and free-radicals scavenging activities, binding and transport of many endogenous and exogenous substances, and regulation of endothelial function and inflammatory response. These non-oncotic activities are closely connected to the peculiarly dynamic structure of the albumin molecule. HSA undergoes spontaneous structural modifications, mainly by reaction with oxidants and saccharides; however, patients with cirrhosis show extensive post-transcriptional changes at several molecular sites of HSA, the degree of which parallels the severity of the disease. The present work reports the development and application of an innovative LC-MS analytical method for a rapid and reproducible determination of the relative abundance of HSA isoforms in plasma samples from alcoholic hepatitis (AH) patients. A condition of severe oxidative stress, similar to that observed in AH patients, is associated with profound changes in circulating HSA microheterogeneity. More interestingly, the high resolution provided by the analytical platform allowed the monitoring of novel oxidative products of HSA never reported before.",
keywords = "Journal Article, Research Support, Non-U.S. Gov't",
author = "Marina Naldi and Maurizio Baldassarre and Marco Domenicali and Giannone, {Ferdinando Antonino} and Matteo Bossi and Jonathan Montomoli and Sandahl, {Thomas Damgaard} and Emilie Glavind and Hendrik Vilstrup and Paolo Caraceni and Carlo Bertucci",
note = "Copyright {\textcopyright} 2016 Elsevier B.V. All rights reserved.",
year = "2016",
month = apr,
day = "15",
doi = "10.1016/j.jpba.2016.01.048",
language = "English",
volume = "122",
pages = "141--7",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
issn = "0731-7085",
publisher = "Elsevier BV",

}

RIS

TY - JOUR

T1 - Mass spectrometry characterization of circulating human serum albumin microheterogeneity in patients with alcoholic hepatitis

AU - Naldi, Marina

AU - Baldassarre, Maurizio

AU - Domenicali, Marco

AU - Giannone, Ferdinando Antonino

AU - Bossi, Matteo

AU - Montomoli, Jonathan

AU - Sandahl, Thomas Damgaard

AU - Glavind, Emilie

AU - Vilstrup, Hendrik

AU - Caraceni, Paolo

AU - Bertucci, Carlo

N1 - Copyright © 2016 Elsevier B.V. All rights reserved.

PY - 2016/4/15

Y1 - 2016/4/15

N2 - Human serum albumin (HSA) is the most abundant plasma protein, endowed with several biological properties unrelated to its oncotic power, such as antioxidant and free-radicals scavenging activities, binding and transport of many endogenous and exogenous substances, and regulation of endothelial function and inflammatory response. These non-oncotic activities are closely connected to the peculiarly dynamic structure of the albumin molecule. HSA undergoes spontaneous structural modifications, mainly by reaction with oxidants and saccharides; however, patients with cirrhosis show extensive post-transcriptional changes at several molecular sites of HSA, the degree of which parallels the severity of the disease. The present work reports the development and application of an innovative LC-MS analytical method for a rapid and reproducible determination of the relative abundance of HSA isoforms in plasma samples from alcoholic hepatitis (AH) patients. A condition of severe oxidative stress, similar to that observed in AH patients, is associated with profound changes in circulating HSA microheterogeneity. More interestingly, the high resolution provided by the analytical platform allowed the monitoring of novel oxidative products of HSA never reported before.

AB - Human serum albumin (HSA) is the most abundant plasma protein, endowed with several biological properties unrelated to its oncotic power, such as antioxidant and free-radicals scavenging activities, binding and transport of many endogenous and exogenous substances, and regulation of endothelial function and inflammatory response. These non-oncotic activities are closely connected to the peculiarly dynamic structure of the albumin molecule. HSA undergoes spontaneous structural modifications, mainly by reaction with oxidants and saccharides; however, patients with cirrhosis show extensive post-transcriptional changes at several molecular sites of HSA, the degree of which parallels the severity of the disease. The present work reports the development and application of an innovative LC-MS analytical method for a rapid and reproducible determination of the relative abundance of HSA isoforms in plasma samples from alcoholic hepatitis (AH) patients. A condition of severe oxidative stress, similar to that observed in AH patients, is associated with profound changes in circulating HSA microheterogeneity. More interestingly, the high resolution provided by the analytical platform allowed the monitoring of novel oxidative products of HSA never reported before.

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.jpba.2016.01.048

DO - 10.1016/j.jpba.2016.01.048

M3 - Journal article

C2 - 26852162

VL - 122

SP - 141

EP - 147

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

SN - 0731-7085

ER -